Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3137 and Pf6N2E2_3138 are separated by 158 nucleotides Pf6N2E2_3138 and Pf6N2E2_3139 are separated by 147 nucleotides Pf6N2E2_3139 and Pf6N2E2_3140 are separated by 234 nucleotides Pf6N2E2_3140 and Pf6N2E2_3141 are separated by 184 nucleotides
Pf6N2E2_3137 - Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-), at 3,628,697 to 3,630,406
_3137
Pf6N2E2_3138 - YaeQ protein, at 3,630,565 to 3,631,107
_3138
Pf6N2E2_3139 - L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16), at 3,631,255 to 3,632,454
_3139
Pf6N2E2_3140 - FIG00953680: hypothetical protein, at 3,632,689 to 3,632,976
_3140
Pf6N2E2_3141 - virulence sensor protein, at 3,633,161 to 3,634,531
_3141
Position (kb)
3631
3632
3633 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3630.330 kb on + strand at 3630.331 kb on - strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.399 kb on - strand at 3630.399 kb on - strand at 3630.399 kb on - strand at 3630.957 kb on - strand, within Pf6N2E2_3138 at 3630.968 kb on + strand, within Pf6N2E2_3138 at 3630.969 kb on - strand, within Pf6N2E2_3138 at 3631.043 kb on + strand, within Pf6N2E2_3138 at 3631.043 kb on + strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.085 kb on + strand at 3631.085 kb on + strand at 3631.085 kb on + strand at 3631.123 kb on + strand at 3631.123 kb on + strand at 3631.123 kb on + strand at 3631.208 kb on - strand at 3631.212 kb on + strand at 3631.212 kb on + strand at 3631.212 kb on + strand at 3631.213 kb on - strand at 3631.213 kb on - strand at 3631.213 kb on - strand at 3631.222 kb on + strand at 3631.415 kb on + strand, within Pf6N2E2_3139 at 3631.415 kb on + strand, within Pf6N2E2_3139 at 3631.415 kb on + strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.418 kb on - strand, within Pf6N2E2_3139 at 3631.418 kb on - strand, within Pf6N2E2_3139 at 3631.418 kb on - strand, within Pf6N2E2_3139 at 3631.712 kb on - strand, within Pf6N2E2_3139 at 3631.864 kb on + strand, within Pf6N2E2_3139 at 3631.865 kb on - strand, within Pf6N2E2_3139 at 3631.865 kb on - strand, within Pf6N2E2_3139 at 3632.125 kb on + strand, within Pf6N2E2_3139 at 3632.126 kb on - strand, within Pf6N2E2_3139 at 3632.229 kb on + strand, within Pf6N2E2_3139 at 3632.229 kb on + strand, within Pf6N2E2_3139 at 3632.230 kb on - strand, within Pf6N2E2_3139 at 3632.522 kb on + strand at 3632.664 kb on + strand at 3632.665 kb on - strand at 3632.665 kb on - strand at 3632.665 kb on - strand at 3632.665 kb on - strand at 3632.776 kb on + strand, within Pf6N2E2_3140 at 3632.777 kb on - strand, within Pf6N2E2_3140 at 3632.777 kb on - strand, within Pf6N2E2_3140 at 3632.777 kb on - strand, within Pf6N2E2_3140 at 3632.934 kb on - strand, within Pf6N2E2_3140 at 3632.959 kb on + strand at 3632.959 kb on + strand at 3632.960 kb on - strand at 3632.967 kb on + strand at 3632.993 kb on - strand at 3633.020 kb on + strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.021 kb on - strand at 3633.106 kb on + strand at 3633.106 kb on + strand at 3633.106 kb on + strand at 3633.106 kb on + strand at 3633.106 kb on + strand at 3633.249 kb on + strand at 3633.249 kb on + strand at 3633.250 kb on - strand at 3633.250 kb on - strand at 3633.250 kb on - strand at 3633.250 kb on - strand at 3633.270 kb on + strand at 3633.271 kb on - strand at 3633.271 kb on - strand at 3633.271 kb on - strand at 3633.438 kb on - strand, within Pf6N2E2_3141 at 3633.451 kb on - strand, within Pf6N2E2_3141
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 3,630,330 + +0.7 3,630,331 - +0.1 3,630,398 + -2.8 3,630,398 + -1.1 3,630,398 + -1.5 3,630,398 + -0.8 3,630,398 + -2.6 3,630,399 - +1.2 3,630,399 - +0.7 3,630,399 - -2.3 3,630,957 - Pf6N2E2_3138 0.72 -1.6 3,630,968 + Pf6N2E2_3138 0.74 -2.8 3,630,969 - Pf6N2E2_3138 0.74 +0.3 3,631,043 + Pf6N2E2_3138 0.88 -1.9 3,631,043 + Pf6N2E2_3138 0.88 -0.1 3,631,044 - Pf6N2E2_3138 0.88 +3.9 3,631,044 - Pf6N2E2_3138 0.88 -2.7 3,631,044 - Pf6N2E2_3138 0.88 -0.0 3,631,044 - Pf6N2E2_3138 0.88 +0.1 3,631,085 + -0.1 3,631,085 + +0.7 3,631,085 + +0.2 3,631,123 + -2.2 3,631,123 + -1.8 3,631,123 + -0.5 3,631,208 - -3.4 3,631,212 + +0.5 3,631,212 + +2.3 3,631,212 + -1.1 3,631,213 - -0.4 3,631,213 - +1.3 3,631,213 - -0.7 3,631,222 + -1.4 3,631,415 + Pf6N2E2_3139 0.13 +0.3 3,631,415 + Pf6N2E2_3139 0.13 +2.1 3,631,415 + Pf6N2E2_3139 0.13 +0.4 3,631,416 - Pf6N2E2_3139 0.13 +1.9 3,631,416 - Pf6N2E2_3139 0.13 +1.2 3,631,416 - Pf6N2E2_3139 0.13 +0.6 3,631,416 - Pf6N2E2_3139 0.13 +0.5 3,631,416 - Pf6N2E2_3139 0.13 +1.1 3,631,416 - Pf6N2E2_3139 0.13 +1.0 3,631,418 - Pf6N2E2_3139 0.14 -1.1 3,631,418 - Pf6N2E2_3139 0.14 +1.2 3,631,418 - Pf6N2E2_3139 0.14 +2.0 3,631,712 - Pf6N2E2_3139 0.38 -2.5 3,631,864 + Pf6N2E2_3139 0.51 +1.2 3,631,865 - Pf6N2E2_3139 0.51 -0.6 3,631,865 - Pf6N2E2_3139 0.51 -0.3 3,632,125 + Pf6N2E2_3139 0.72 -2.3 3,632,126 - Pf6N2E2_3139 0.73 +2.1 3,632,229 + Pf6N2E2_3139 0.81 -0.5 3,632,229 + Pf6N2E2_3139 0.81 +0.9 3,632,230 - Pf6N2E2_3139 0.81 +1.7 3,632,522 + +1.0 3,632,664 + +1.1 3,632,665 - +0.9 3,632,665 - -3.2 3,632,665 - +0.5 3,632,665 - +1.2 3,632,776 + Pf6N2E2_3140 0.30 -2.2 3,632,777 - Pf6N2E2_3140 0.31 -1.4 3,632,777 - Pf6N2E2_3140 0.31 +0.7 3,632,777 - Pf6N2E2_3140 0.31 +1.5 3,632,934 - Pf6N2E2_3140 0.85 +1.1 3,632,959 + +0.6 3,632,959 + -2.2 3,632,960 - -1.5 3,632,967 + -3.0 3,632,993 - -0.5 3,633,020 + -1.9 3,633,021 - +1.8 3,633,021 - +1.2 3,633,021 - -0.2 3,633,021 - -0.6 3,633,021 - +2.5 3,633,021 - +2.1 3,633,021 - -1.1 3,633,106 + +1.3 3,633,106 + -0.0 3,633,106 + +0.4 3,633,106 + +0.4 3,633,106 + -0.5 3,633,249 + +1.5 3,633,249 + -2.5 3,633,250 - -1.1 3,633,250 - +0.5 3,633,250 - +1.2 3,633,250 - +0.9 3,633,270 + -2.5 3,633,271 - -1.9 3,633,271 - +1.7 3,633,271 - +1.4 3,633,438 - Pf6N2E2_3141 0.20 -0.2 3,633,451 - Pf6N2E2_3141 0.21 -2.2
Or see this region's nucleotide sequence