Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3026

Experiment: Bile salts 0.0125 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3024 and Pf6N2E2_3025 overlap by 4 nucleotidesPf6N2E2_3025 and Pf6N2E2_3026 overlap by 4 nucleotidesPf6N2E2_3026 and Pf6N2E2_3027 are separated by 11 nucleotidesPf6N2E2_3027 and Pf6N2E2_3028 are separated by 3 nucleotides Pf6N2E2_3024 - Phage tail fibers, at 3,510,612 to 3,511,247 _3024 Pf6N2E2_3025 - Baseplate assembly protein J, at 3,511,244 to 3,512,239 _3025 Pf6N2E2_3026 - Baseplate assembly protein W, at 3,512,236 to 3,512,568 _3026 Pf6N2E2_3027 - Baseplate assembly protein V, at 3,512,580 to 3,513,191 _3027 Pf6N2E2_3028 - FIG00959937: hypothetical protein, at 3,513,195 to 3,513,707 _3028 Position (kb) 3512 3513Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3511.247 kb on + strandat 3511.248 kb on - strandat 3511.366 kb on - strand, within Pf6N2E2_3025at 3511.419 kb on + strand, within Pf6N2E2_3025at 3511.420 kb on - strand, within Pf6N2E2_3025at 3511.504 kb on - strand, within Pf6N2E2_3025at 3511.716 kb on + strand, within Pf6N2E2_3025at 3511.716 kb on + strand, within Pf6N2E2_3025at 3511.716 kb on + strand, within Pf6N2E2_3025at 3511.717 kb on - strand, within Pf6N2E2_3025at 3511.717 kb on - strand, within Pf6N2E2_3025at 3511.730 kb on + strand, within Pf6N2E2_3025at 3511.730 kb on + strand, within Pf6N2E2_3025at 3511.730 kb on + strand, within Pf6N2E2_3025at 3511.731 kb on - strand, within Pf6N2E2_3025at 3511.780 kb on + strand, within Pf6N2E2_3025at 3511.840 kb on - strand, within Pf6N2E2_3025at 3511.840 kb on - strand, within Pf6N2E2_3025at 3511.873 kb on - strand, within Pf6N2E2_3025at 3512.010 kb on + strand, within Pf6N2E2_3025at 3512.010 kb on + strand, within Pf6N2E2_3025at 3512.011 kb on - strand, within Pf6N2E2_3025at 3512.011 kb on - strand, within Pf6N2E2_3025at 3512.011 kb on - strand, within Pf6N2E2_3025at 3512.133 kb on + strand, within Pf6N2E2_3025at 3512.133 kb on + strand, within Pf6N2E2_3025at 3512.133 kb on + strand, within Pf6N2E2_3025at 3512.134 kb on - strand, within Pf6N2E2_3025at 3512.366 kb on + strand, within Pf6N2E2_3026at 3512.367 kb on - strand, within Pf6N2E2_3026at 3512.453 kb on + strand, within Pf6N2E2_3026at 3512.453 kb on + strand, within Pf6N2E2_3026at 3512.454 kb on - strand, within Pf6N2E2_3026at 3512.454 kb on - strand, within Pf6N2E2_3026at 3512.454 kb on - strand, within Pf6N2E2_3026at 3512.454 kb on - strand, within Pf6N2E2_3026at 3512.454 kb on - strand, within Pf6N2E2_3026at 3512.512 kb on + strand, within Pf6N2E2_3026at 3512.512 kb on + strand, within Pf6N2E2_3026at 3512.548 kb on - strandat 3512.582 kb on - strandat 3512.721 kb on - strand, within Pf6N2E2_3027at 3513.214 kb on + strandat 3513.214 kb on + strandat 3513.214 kb on + strandat 3513.240 kb on + strandat 3513.252 kb on - strand, within Pf6N2E2_3028at 3513.305 kb on - strand, within Pf6N2E2_3028at 3513.340 kb on + strand, within Pf6N2E2_3028at 3513.341 kb on - strand, within Pf6N2E2_3028at 3513.341 kb on - strand, within Pf6N2E2_3028at 3513.341 kb on - strand, within Pf6N2E2_3028at 3513.341 kb on - strand, within Pf6N2E2_3028

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Per-strain Table

Position Strand Gene LocusTag Fraction Bile salts 0.0125 M
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3,511,247 + +1.4
3,511,248 - +0.3
3,511,366 - Pf6N2E2_3025 0.12 +0.0
3,511,419 + Pf6N2E2_3025 0.18 +2.6
3,511,420 - Pf6N2E2_3025 0.18 +0.0
3,511,504 - Pf6N2E2_3025 0.26 -0.8
3,511,716 + Pf6N2E2_3025 0.47 -2.3
3,511,716 + Pf6N2E2_3025 0.47 +1.5
3,511,716 + Pf6N2E2_3025 0.47 +0.0
3,511,717 - Pf6N2E2_3025 0.47 +1.1
3,511,717 - Pf6N2E2_3025 0.47 +1.5
3,511,730 + Pf6N2E2_3025 0.49 -1.2
3,511,730 + Pf6N2E2_3025 0.49 +1.9
3,511,730 + Pf6N2E2_3025 0.49 +0.3
3,511,731 - Pf6N2E2_3025 0.49 -2.0
3,511,780 + Pf6N2E2_3025 0.54 -1.3
3,511,840 - Pf6N2E2_3025 0.60 +0.4
3,511,840 - Pf6N2E2_3025 0.60 -2.7
3,511,873 - Pf6N2E2_3025 0.63 -3.2
3,512,010 + Pf6N2E2_3025 0.77 -2.5
3,512,010 + Pf6N2E2_3025 0.77 -2.7
3,512,011 - Pf6N2E2_3025 0.77 -0.6
3,512,011 - Pf6N2E2_3025 0.77 +0.3
3,512,011 - Pf6N2E2_3025 0.77 +0.4
3,512,133 + Pf6N2E2_3025 0.89 +0.1
3,512,133 + Pf6N2E2_3025 0.89 -0.8
3,512,133 + Pf6N2E2_3025 0.89 +0.6
3,512,134 - Pf6N2E2_3025 0.89 -2.1
3,512,366 + Pf6N2E2_3026 0.39 -0.2
3,512,367 - Pf6N2E2_3026 0.39 +0.4
3,512,453 + Pf6N2E2_3026 0.65 +1.8
3,512,453 + Pf6N2E2_3026 0.65 -2.2
3,512,454 - Pf6N2E2_3026 0.65 +1.5
3,512,454 - Pf6N2E2_3026 0.65 +0.3
3,512,454 - Pf6N2E2_3026 0.65 +1.2
3,512,454 - Pf6N2E2_3026 0.65 +1.9
3,512,454 - Pf6N2E2_3026 0.65 -2.5
3,512,512 + Pf6N2E2_3026 0.83 +0.0
3,512,512 + Pf6N2E2_3026 0.83 +0.5
3,512,548 - +1.2
3,512,582 - +2.0
3,512,721 - Pf6N2E2_3027 0.23 +0.9
3,513,214 + -1.4
3,513,214 + -0.1
3,513,214 + +1.0
3,513,240 + +0.8
3,513,252 - Pf6N2E2_3028 0.11 +1.0
3,513,305 - Pf6N2E2_3028 0.21 -3.5
3,513,340 + Pf6N2E2_3028 0.28 -0.6
3,513,341 - Pf6N2E2_3028 0.28 +0.5
3,513,341 - Pf6N2E2_3028 0.28 +0.3
3,513,341 - Pf6N2E2_3028 0.28 +0.1
3,513,341 - Pf6N2E2_3028 0.28 +0.1

Or see this region's nucleotide sequence