Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3020 and Pf6N2E2_3021 are separated by 2 nucleotides Pf6N2E2_3021 and Pf6N2E2_3022 are separated by 90 nucleotides Pf6N2E2_3022 and Pf6N2E2_3023 are separated by 410 nucleotides
Pf6N2E2_3020 - Phage tail sheath monomer, at 3,506,255 to 3,507,421
_3020
Pf6N2E2_3021 - hypothetical protein, at 3,507,424 to 3,507,633
_3021
Pf6N2E2_3022 - hypothetical protein, at 3,507,724 to 3,508,122
_3022
Pf6N2E2_3023 - Prophage long tail fiber protein, at 3,508,533 to 3,510,611
_3023
Position (kb)
3507
3508
3509 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3506.731 kb on - strand, within Pf6N2E2_3020 at 3506.731 kb on - strand, within Pf6N2E2_3020 at 3506.835 kb on + strand, within Pf6N2E2_3020 at 3506.835 kb on + strand, within Pf6N2E2_3020 at 3506.836 kb on - strand, within Pf6N2E2_3020 at 3506.836 kb on - strand, within Pf6N2E2_3020 at 3506.907 kb on + strand, within Pf6N2E2_3020 at 3506.907 kb on + strand, within Pf6N2E2_3020 at 3506.907 kb on + strand, within Pf6N2E2_3020 at 3506.908 kb on - strand, within Pf6N2E2_3020 at 3506.908 kb on - strand, within Pf6N2E2_3020 at 3506.908 kb on - strand, within Pf6N2E2_3020 at 3506.908 kb on - strand, within Pf6N2E2_3020 at 3506.908 kb on - strand, within Pf6N2E2_3020 at 3506.908 kb on - strand, within Pf6N2E2_3020 at 3506.908 kb on - strand, within Pf6N2E2_3020 at 3507.162 kb on + strand, within Pf6N2E2_3020 at 3507.425 kb on + strand at 3507.607 kb on + strand, within Pf6N2E2_3021 at 3507.614 kb on + strand at 3507.614 kb on + strand at 3507.614 kb on + strand at 3507.614 kb on + strand at 3507.615 kb on - strand at 3507.726 kb on - strand at 3508.046 kb on - strand, within Pf6N2E2_3022 at 3508.109 kb on - strand at 3508.109 kb on - strand at 3508.122 kb on + strand at 3508.123 kb on - strand at 3508.140 kb on + strand at 3508.140 kb on + strand at 3508.140 kb on + strand at 3508.141 kb on - strand at 3508.141 kb on - strand at 3508.143 kb on + strand at 3508.429 kb on + strand at 3508.430 kb on - strand at 3508.498 kb on + strand at 3508.499 kb on - strand at 3508.534 kb on + strand at 3508.535 kb on - strand at 3508.535 kb on - strand at 3508.535 kb on - strand at 3508.535 kb on - strand at 3508.537 kb on + strand at 3508.538 kb on - strand at 3508.538 kb on - strand at 3508.663 kb on + strand at 3508.663 kb on + strand at 3508.684 kb on + strand at 3508.704 kb on + strand at 3508.704 kb on + strand at 3508.705 kb on - strand at 3508.705 kb on - strand at 3508.705 kb on - strand at 3508.705 kb on - strand at 3508.705 kb on - strand at 3508.705 kb on - strand at 3508.705 kb on - strand at 3508.772 kb on - strand, within Pf6N2E2_3023 at 3508.772 kb on - strand, within Pf6N2E2_3023 at 3508.838 kb on + strand, within Pf6N2E2_3023 at 3508.838 kb on + strand, within Pf6N2E2_3023 at 3508.838 kb on + strand, within Pf6N2E2_3023 at 3508.838 kb on + strand, within Pf6N2E2_3023 at 3508.838 kb on + strand, within Pf6N2E2_3023 at 3508.838 kb on + strand, within Pf6N2E2_3023 at 3508.839 kb on - strand, within Pf6N2E2_3023 at 3508.839 kb on - strand, within Pf6N2E2_3023 at 3508.839 kb on - strand, within Pf6N2E2_3023 at 3508.839 kb on - strand, within Pf6N2E2_3023 at 3508.839 kb on - strand, within Pf6N2E2_3023 at 3508.839 kb on - strand, within Pf6N2E2_3023 at 3508.839 kb on - strand, within Pf6N2E2_3023 at 3508.839 kb on - strand, within Pf6N2E2_3023 at 3508.857 kb on + strand, within Pf6N2E2_3023 at 3508.857 kb on + strand, within Pf6N2E2_3023 at 3508.857 kb on + strand, within Pf6N2E2_3023 at 3508.857 kb on + strand, within Pf6N2E2_3023 at 3508.858 kb on - strand, within Pf6N2E2_3023 at 3508.858 kb on - strand, within Pf6N2E2_3023 at 3508.858 kb on - strand, within Pf6N2E2_3023 at 3508.865 kb on + strand, within Pf6N2E2_3023 at 3508.866 kb on - strand, within Pf6N2E2_3023 at 3508.866 kb on - strand, within Pf6N2E2_3023 at 3508.956 kb on - strand, within Pf6N2E2_3023 at 3508.976 kb on + strand, within Pf6N2E2_3023 at 3509.018 kb on + strand, within Pf6N2E2_3023 at 3509.105 kb on + strand, within Pf6N2E2_3023 at 3509.106 kb on - strand, within Pf6N2E2_3023 at 3509.106 kb on - strand, within Pf6N2E2_3023
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 3,506,731 - Pf6N2E2_3020 0.41 -2.0 3,506,731 - Pf6N2E2_3020 0.41 -0.8 3,506,835 + Pf6N2E2_3020 0.50 -1.7 3,506,835 + Pf6N2E2_3020 0.50 -1.5 3,506,836 - Pf6N2E2_3020 0.50 +1.3 3,506,836 - Pf6N2E2_3020 0.50 -1.6 3,506,907 + Pf6N2E2_3020 0.56 +1.1 3,506,907 + Pf6N2E2_3020 0.56 +0.1 3,506,907 + Pf6N2E2_3020 0.56 +0.5 3,506,908 - Pf6N2E2_3020 0.56 -1.2 3,506,908 - Pf6N2E2_3020 0.56 +1.5 3,506,908 - Pf6N2E2_3020 0.56 -0.0 3,506,908 - Pf6N2E2_3020 0.56 +1.7 3,506,908 - Pf6N2E2_3020 0.56 +0.8 3,506,908 - Pf6N2E2_3020 0.56 -1.0 3,506,908 - Pf6N2E2_3020 0.56 +1.1 3,507,162 + Pf6N2E2_3020 0.78 -1.4 3,507,425 + +1.5 3,507,607 + Pf6N2E2_3021 0.87 -2.8 3,507,614 + +0.6 3,507,614 + +1.0 3,507,614 + -1.4 3,507,614 + -0.8 3,507,615 - -0.3 3,507,726 - +0.4 3,508,046 - Pf6N2E2_3022 0.81 -0.3 3,508,109 - +0.4 3,508,109 - +0.7 3,508,122 + -1.1 3,508,123 - +0.8 3,508,140 + -1.6 3,508,140 + +2.9 3,508,140 + -2.3 3,508,141 - +0.4 3,508,141 - -2.2 3,508,143 + +0.6 3,508,429 + -2.3 3,508,430 - +2.9 3,508,498 + -2.2 3,508,499 - -0.3 3,508,534 + -1.7 3,508,535 - -2.3 3,508,535 - +0.0 3,508,535 - -3.4 3,508,535 - -3.0 3,508,537 + +1.5 3,508,538 - +1.6 3,508,538 - -2.0 3,508,663 + +1.2 3,508,663 + +3.1 3,508,684 + -1.0 3,508,704 + -1.3 3,508,704 + +0.9 3,508,705 - -0.6 3,508,705 - +0.8 3,508,705 - +1.0 3,508,705 - +1.7 3,508,705 - -0.8 3,508,705 - -2.7 3,508,705 - +0.4 3,508,772 - Pf6N2E2_3023 0.11 +0.5 3,508,772 - Pf6N2E2_3023 0.11 -2.7 3,508,838 + Pf6N2E2_3023 0.15 +1.6 3,508,838 + Pf6N2E2_3023 0.15 -2.7 3,508,838 + Pf6N2E2_3023 0.15 -1.1 3,508,838 + Pf6N2E2_3023 0.15 +1.7 3,508,838 + Pf6N2E2_3023 0.15 -2.4 3,508,838 + Pf6N2E2_3023 0.15 -1.8 3,508,839 - Pf6N2E2_3023 0.15 +1.7 3,508,839 - Pf6N2E2_3023 0.15 -2.0 3,508,839 - Pf6N2E2_3023 0.15 -0.4 3,508,839 - Pf6N2E2_3023 0.15 +1.1 3,508,839 - Pf6N2E2_3023 0.15 -0.4 3,508,839 - Pf6N2E2_3023 0.15 +1.2 3,508,839 - Pf6N2E2_3023 0.15 -0.7 3,508,839 - Pf6N2E2_3023 0.15 -1.9 3,508,857 + Pf6N2E2_3023 0.16 -1.0 3,508,857 + Pf6N2E2_3023 0.16 +2.5 3,508,857 + Pf6N2E2_3023 0.16 -1.8 3,508,857 + Pf6N2E2_3023 0.16 -1.3 3,508,858 - Pf6N2E2_3023 0.16 -2.7 3,508,858 - Pf6N2E2_3023 0.16 +0.4 3,508,858 - Pf6N2E2_3023 0.16 -1.4 3,508,865 + Pf6N2E2_3023 0.16 -0.6 3,508,866 - Pf6N2E2_3023 0.16 -3.7 3,508,866 - Pf6N2E2_3023 0.16 +0.9 3,508,956 - Pf6N2E2_3023 0.20 +0.8 3,508,976 + Pf6N2E2_3023 0.21 -0.8 3,509,018 + Pf6N2E2_3023 0.23 -0.9 3,509,105 + Pf6N2E2_3023 0.28 -3.2 3,509,106 - Pf6N2E2_3023 0.28 -2.7 3,509,106 - Pf6N2E2_3023 0.28 -0.4
Or see this region's nucleotide sequence