Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_255 and Pf6N2E2_256 overlap by 8 nucleotides Pf6N2E2_256 and Pf6N2E2_257 are separated by 118 nucleotides Pf6N2E2_257 and Pf6N2E2_258 are separated by 145 nucleotides
Pf6N2E2_255 - DoxX family protein, at 319,191 to 319,604
_255
Pf6N2E2_256 - putative membrane protein, at 319,597 to 320,235
_256
Pf6N2E2_257 - Exoenzymes regulatory protein AepA precursor, at 320,354 to 322,192
_257
Pf6N2E2_258 - Nicotinamidase family protein YcaC, at 322,338 to 322,967
_258
Position (kb) 320
321
322
323 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 319.525 kb on + strand, within Pf6N2E2_255 at 319.525 kb on + strand, within Pf6N2E2_255 at 319.526 kb on - strand, within Pf6N2E2_255 at 319.526 kb on - strand, within Pf6N2E2_255 at 319.537 kb on + strand, within Pf6N2E2_255 at 319.537 kb on + strand, within Pf6N2E2_255 at 319.538 kb on - strand, within Pf6N2E2_255 at 319.538 kb on - strand, within Pf6N2E2_255 at 319.733 kb on + strand, within Pf6N2E2_256 at 319.734 kb on - strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.092 kb on + strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.093 kb on - strand, within Pf6N2E2_256 at 320.143 kb on + strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.144 kb on - strand, within Pf6N2E2_256 at 320.333 kb on - strand at 320.393 kb on - strand at 320.790 kb on - strand, within Pf6N2E2_257 at 320.834 kb on + strand, within Pf6N2E2_257 at 320.975 kb on + strand, within Pf6N2E2_257 at 321.077 kb on + strand, within Pf6N2E2_257 at 321.078 kb on - strand, within Pf6N2E2_257 at 321.131 kb on + strand, within Pf6N2E2_257 at 321.132 kb on - strand, within Pf6N2E2_257 at 321.383 kb on + strand, within Pf6N2E2_257 at 321.383 kb on + strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.384 kb on - strand, within Pf6N2E2_257 at 321.579 kb on - strand, within Pf6N2E2_257 at 321.579 kb on - strand, within Pf6N2E2_257 at 321.677 kb on + strand, within Pf6N2E2_257 at 321.678 kb on - strand, within Pf6N2E2_257 at 321.678 kb on - strand, within Pf6N2E2_257 at 321.678 kb on - strand, within Pf6N2E2_257 at 321.699 kb on - strand, within Pf6N2E2_257 at 321.947 kb on + strand, within Pf6N2E2_257 at 321.948 kb on - strand, within Pf6N2E2_257 at 322.311 kb on + strand at 322.312 kb on - strand at 322.312 kb on - strand at 322.356 kb on + strand at 322.584 kb on + strand, within Pf6N2E2_258 at 322.584 kb on + strand, within Pf6N2E2_258 at 322.584 kb on + strand, within Pf6N2E2_258 at 322.584 kb on + strand, within Pf6N2E2_258 at 322.584 kb on + strand, within Pf6N2E2_258 at 322.584 kb on + strand, within Pf6N2E2_258 at 322.767 kb on - strand, within Pf6N2E2_258 at 322.944 kb on + strand at 322.944 kb on + strand at 322.945 kb on - strand at 322.945 kb on - strand at 322.965 kb on + strand at 322.966 kb on - strand at 322.966 kb on - strand at 322.966 kb on - strand at 322.966 kb on - strand at 323.026 kb on + strand at 323.027 kb on - strand at 323.027 kb on - strand at 323.047 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 319,525 + Pf6N2E2_255 0.81 -0.2 319,525 + Pf6N2E2_255 0.81 +0.8 319,526 - Pf6N2E2_255 0.81 -2.1 319,526 - Pf6N2E2_255 0.81 +2.5 319,537 + Pf6N2E2_255 0.84 +0.3 319,537 + Pf6N2E2_255 0.84 -0.2 319,538 - Pf6N2E2_255 0.84 +2.3 319,538 - Pf6N2E2_255 0.84 -0.7 319,733 + Pf6N2E2_256 0.21 +0.7 319,734 - Pf6N2E2_256 0.21 -1.8 320,092 + Pf6N2E2_256 0.77 -1.6 320,092 + Pf6N2E2_256 0.77 -0.3 320,092 + Pf6N2E2_256 0.77 -1.2 320,092 + Pf6N2E2_256 0.77 -0.8 320,093 - Pf6N2E2_256 0.78 +0.1 320,093 - Pf6N2E2_256 0.78 +0.6 320,093 - Pf6N2E2_256 0.78 -0.6 320,093 - Pf6N2E2_256 0.78 +0.5 320,143 + Pf6N2E2_256 0.85 -1.9 320,144 - Pf6N2E2_256 0.86 -0.6 320,144 - Pf6N2E2_256 0.86 +2.1 320,144 - Pf6N2E2_256 0.86 -1.4 320,144 - Pf6N2E2_256 0.86 +0.4 320,144 - Pf6N2E2_256 0.86 -0.2 320,333 - +0.3 320,393 - +0.6 320,790 - Pf6N2E2_257 0.24 -1.7 320,834 + Pf6N2E2_257 0.26 +0.9 320,975 + Pf6N2E2_257 0.34 -0.6 321,077 + Pf6N2E2_257 0.39 -0.6 321,078 - Pf6N2E2_257 0.39 -2.3 321,131 + Pf6N2E2_257 0.42 +0.9 321,132 - Pf6N2E2_257 0.42 +0.8 321,383 + Pf6N2E2_257 0.56 -0.4 321,383 + Pf6N2E2_257 0.56 -0.2 321,384 - Pf6N2E2_257 0.56 -1.1 321,384 - Pf6N2E2_257 0.56 -2.9 321,384 - Pf6N2E2_257 0.56 +1.5 321,384 - Pf6N2E2_257 0.56 -0.6 321,384 - Pf6N2E2_257 0.56 -1.1 321,384 - Pf6N2E2_257 0.56 -0.9 321,579 - Pf6N2E2_257 0.67 -0.6 321,579 - Pf6N2E2_257 0.67 +0.7 321,677 + Pf6N2E2_257 0.72 +1.7 321,678 - Pf6N2E2_257 0.72 -1.8 321,678 - Pf6N2E2_257 0.72 +0.0 321,678 - Pf6N2E2_257 0.72 +0.5 321,699 - Pf6N2E2_257 0.73 +0.4 321,947 + Pf6N2E2_257 0.87 +0.9 321,948 - Pf6N2E2_257 0.87 -0.6 322,311 + +1.7 322,312 - -3.0 322,312 - +2.4 322,356 + +1.8 322,584 + Pf6N2E2_258 0.39 -0.4 322,584 + Pf6N2E2_258 0.39 +0.3 322,584 + Pf6N2E2_258 0.39 -2.6 322,584 + Pf6N2E2_258 0.39 -0.1 322,584 + Pf6N2E2_258 0.39 +1.6 322,584 + Pf6N2E2_258 0.39 +0.4 322,767 - Pf6N2E2_258 0.68 +2.2 322,944 + +0.1 322,944 + +1.3 322,945 - +0.5 322,945 - -1.3 322,965 + +2.5 322,966 - +0.7 322,966 - +0.6 322,966 - +0.8 322,966 - -1.3 323,026 + +0.0 323,027 - -1.4 323,027 - -1.6 323,047 + +1.9
Or see this region's nucleotide sequence