Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_240 and Pf6N2E2_241 are separated by 482 nucleotides Pf6N2E2_241 and Pf6N2E2_242 overlap by 1 nucleotides Pf6N2E2_242 and Pf6N2E2_243 are separated by 49 nucleotides Pf6N2E2_243 and Pf6N2E2_244 are separated by 36 nucleotides
Pf6N2E2_240 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 301,003 to 301,893
_240
Pf6N2E2_241 - hypothetical protein, at 302,376 to 302,519
_241
Pf6N2E2_242 - Glycine betaine ABC transport system permease protein, at 302,519 to 303,202
_242
Pf6N2E2_243 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 303,252 to 304,157
_243
Pf6N2E2_244 - Nitrilotriacetate monooxygenase component A (EC 1.14.13.-), at 304,194 to 305,522
_244
Position (kb) 302
303
304 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 301.641 kb on + strand, within Pf6N2E2_240 at 301.642 kb on - strand, within Pf6N2E2_240 at 301.952 kb on + strand at 301.981 kb on + strand at 302.115 kb on - strand at 302.157 kb on + strand at 302.157 kb on + strand at 302.157 kb on + strand at 302.244 kb on + strand at 302.336 kb on - strand at 302.481 kb on + strand, within Pf6N2E2_241 at 302.540 kb on + strand at 302.540 kb on + strand at 302.612 kb on + strand, within Pf6N2E2_242 at 302.677 kb on + strand, within Pf6N2E2_242 at 302.682 kb on - strand, within Pf6N2E2_242 at 302.840 kb on - strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.843 kb on + strand, within Pf6N2E2_242 at 302.844 kb on - strand, within Pf6N2E2_242 at 302.844 kb on - strand, within Pf6N2E2_242 at 302.989 kb on + strand, within Pf6N2E2_242 at 302.989 kb on + strand, within Pf6N2E2_242 at 302.989 kb on + strand, within Pf6N2E2_242 at 302.990 kb on - strand, within Pf6N2E2_242 at 303.196 kb on + strand at 303.405 kb on + strand, within Pf6N2E2_243 at 303.405 kb on + strand, within Pf6N2E2_243 at 303.405 kb on + strand, within Pf6N2E2_243 at 303.405 kb on + strand, within Pf6N2E2_243 at 303.406 kb on - strand, within Pf6N2E2_243 at 303.519 kb on + strand, within Pf6N2E2_243 at 303.520 kb on - strand, within Pf6N2E2_243 at 303.520 kb on - strand, within Pf6N2E2_243 at 303.523 kb on + strand, within Pf6N2E2_243 at 303.523 kb on + strand, within Pf6N2E2_243 at 303.523 kb on + strand, within Pf6N2E2_243 at 303.523 kb on + strand, within Pf6N2E2_243 at 303.523 kb on + strand, within Pf6N2E2_243 at 303.523 kb on + strand, within Pf6N2E2_243 at 303.523 kb on + strand, within Pf6N2E2_243 at 303.647 kb on + strand, within Pf6N2E2_243 at 303.648 kb on - strand, within Pf6N2E2_243 at 303.687 kb on + strand, within Pf6N2E2_243 at 303.773 kb on - strand, within Pf6N2E2_243 at 303.799 kb on - strand, within Pf6N2E2_243 at 303.974 kb on + strand, within Pf6N2E2_243 at 304.136 kb on + strand at 304.176 kb on + strand at 304.176 kb on + strand at 304.177 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 301,641 + Pf6N2E2_240 0.72 +1.3 301,642 - Pf6N2E2_240 0.72 +4.9 301,952 + -1.2 301,981 + +0.4 302,115 - +0.9 302,157 + -2.7 302,157 + +1.0 302,157 + +0.1 302,244 + -0.6 302,336 - -1.9 302,481 + Pf6N2E2_241 0.73 -0.6 302,540 + +0.3 302,540 + -1.4 302,612 + Pf6N2E2_242 0.14 +1.9 302,677 + Pf6N2E2_242 0.23 +1.5 302,682 - Pf6N2E2_242 0.24 -0.9 302,840 - Pf6N2E2_242 0.47 +0.9 302,843 + Pf6N2E2_242 0.47 +0.8 302,843 + Pf6N2E2_242 0.47 -2.5 302,843 + Pf6N2E2_242 0.47 -1.1 302,843 + Pf6N2E2_242 0.47 +0.9 302,843 + Pf6N2E2_242 0.47 +0.2 302,843 + Pf6N2E2_242 0.47 -0.4 302,843 + Pf6N2E2_242 0.47 +1.4 302,843 + Pf6N2E2_242 0.47 +0.8 302,844 - Pf6N2E2_242 0.48 -2.6 302,844 - Pf6N2E2_242 0.48 +1.9 302,989 + Pf6N2E2_242 0.69 +2.6 302,989 + Pf6N2E2_242 0.69 +1.6 302,989 + Pf6N2E2_242 0.69 +2.7 302,990 - Pf6N2E2_242 0.69 -2.7 303,196 + -0.3 303,405 + Pf6N2E2_243 0.17 -0.0 303,405 + Pf6N2E2_243 0.17 -0.5 303,405 + Pf6N2E2_243 0.17 +2.3 303,405 + Pf6N2E2_243 0.17 +0.4 303,406 - Pf6N2E2_243 0.17 +2.8 303,519 + Pf6N2E2_243 0.29 +2.4 303,520 - Pf6N2E2_243 0.30 +2.0 303,520 - Pf6N2E2_243 0.30 +0.7 303,523 + Pf6N2E2_243 0.30 +0.1 303,523 + Pf6N2E2_243 0.30 -3.2 303,523 + Pf6N2E2_243 0.30 -1.6 303,523 + Pf6N2E2_243 0.30 +2.4 303,523 + Pf6N2E2_243 0.30 +0.5 303,523 + Pf6N2E2_243 0.30 -0.4 303,523 + Pf6N2E2_243 0.30 +0.4 303,647 + Pf6N2E2_243 0.44 +0.9 303,648 - Pf6N2E2_243 0.44 +1.7 303,687 + Pf6N2E2_243 0.48 -1.4 303,773 - Pf6N2E2_243 0.58 -1.5 303,799 - Pf6N2E2_243 0.60 +2.0 303,974 + Pf6N2E2_243 0.80 -2.4 304,136 + -1.6 304,176 + -1.7 304,176 + +1.1 304,177 - -1.1
Or see this region's nucleotide sequence