Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2359 and Pf6N2E2_2360 are separated by 255 nucleotides Pf6N2E2_2360 and Pf6N2E2_2361 are separated by 47 nucleotides
Pf6N2E2_2359 - hypothetical protein, at 2,793,234 to 2,794,343
_2359
Pf6N2E2_2360 - cell invasion protein SipB, at 2,794,599 to 2,796,335
_2360
Pf6N2E2_2361 - Type III secretion chaperone protein for YopD (SycD), at 2,796,383 to 2,796,865
_2361
Position (kb)
2794
2795
2796
2797 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.668 kb on - strand, within Pf6N2E2_2359 at 2793.668 kb on - strand, within Pf6N2E2_2359 at 2793.934 kb on + strand, within Pf6N2E2_2359 at 2793.934 kb on + strand, within Pf6N2E2_2359 at 2793.934 kb on + strand, within Pf6N2E2_2359 at 2794.112 kb on + strand at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.116 kb on - strand, within Pf6N2E2_2359 at 2794.116 kb on - strand, within Pf6N2E2_2359 at 2794.261 kb on - strand at 2794.589 kb on + strand at 2794.589 kb on + strand at 2794.589 kb on + strand at 2794.589 kb on + strand at 2794.590 kb on - strand at 2794.590 kb on - strand at 2794.590 kb on - strand at 2794.593 kb on + strand at 2794.594 kb on - strand at 2794.601 kb on - strand at 2794.735 kb on + strand at 2794.736 kb on - strand at 2795.210 kb on - strand, within Pf6N2E2_2360 at 2795.305 kb on - strand, within Pf6N2E2_2360 at 2795.476 kb on + strand at 2795.477 kb on - strand, within Pf6N2E2_2360 at 2795.477 kb on - strand, within Pf6N2E2_2360 at 2795.480 kb on + strand, within Pf6N2E2_2360 at 2795.481 kb on - strand, within Pf6N2E2_2360 at 2795.500 kb on + strand, within Pf6N2E2_2360 at 2795.500 kb on + strand, within Pf6N2E2_2360 at 2795.501 kb on - strand, within Pf6N2E2_2360 at 2795.717 kb on + strand, within Pf6N2E2_2360 at 2795.718 kb on - strand, within Pf6N2E2_2360 at 2795.718 kb on - strand, within Pf6N2E2_2360 at 2795.718 kb on - strand, within Pf6N2E2_2360 at 2796.188 kb on + strand at 2796.221 kb on - strand at 2796.268 kb on + strand at 2796.269 kb on - strand at 2796.269 kb on - strand at 2796.369 kb on + strand at 2796.369 kb on + strand at 2796.369 kb on + strand at 2796.369 kb on + strand at 2796.369 kb on + strand at 2796.369 kb on + strand at 2796.370 kb on - strand at 2796.370 kb on - strand at 2796.370 kb on - strand at 2796.370 kb on - strand at 2796.370 kb on - strand at 2796.370 kb on - strand at 2796.429 kb on + strand at 2796.430 kb on - strand at 2796.430 kb on - strand at 2796.430 kb on - strand at 2796.430 kb on - strand at 2796.480 kb on + strand, within Pf6N2E2_2361 at 2796.481 kb on - strand, within Pf6N2E2_2361 at 2796.481 kb on - strand, within Pf6N2E2_2361 at 2796.481 kb on - strand, within Pf6N2E2_2361 at 2796.540 kb on + strand, within Pf6N2E2_2361 at 2796.541 kb on - strand, within Pf6N2E2_2361 at 2796.589 kb on - strand, within Pf6N2E2_2361 at 2796.589 kb on - strand, within Pf6N2E2_2361 at 2796.651 kb on + strand, within Pf6N2E2_2361 at 2796.663 kb on + strand, within Pf6N2E2_2361 at 2796.663 kb on + strand, within Pf6N2E2_2361 at 2796.663 kb on + strand, within Pf6N2E2_2361 at 2796.663 kb on + strand, within Pf6N2E2_2361 at 2796.664 kb on - strand, within Pf6N2E2_2361 at 2796.673 kb on - strand, within Pf6N2E2_2361 at 2796.726 kb on + strand, within Pf6N2E2_2361 at 2796.726 kb on + strand, within Pf6N2E2_2361 at 2796.727 kb on - strand, within Pf6N2E2_2361 at 2796.727 kb on - strand, within Pf6N2E2_2361 at 2796.727 kb on - strand, within Pf6N2E2_2361 at 2796.727 kb on - strand, within Pf6N2E2_2361 at 2796.747 kb on + strand, within Pf6N2E2_2361 at 2797.249 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 2,793,667 + Pf6N2E2_2359 0.39 -1.9 2,793,667 + Pf6N2E2_2359 0.39 -1.7 2,793,667 + Pf6N2E2_2359 0.39 +0.4 2,793,667 + Pf6N2E2_2359 0.39 +1.1 2,793,668 - Pf6N2E2_2359 0.39 +1.3 2,793,668 - Pf6N2E2_2359 0.39 +0.8 2,793,934 + Pf6N2E2_2359 0.63 +0.1 2,793,934 + Pf6N2E2_2359 0.63 -1.8 2,793,934 + Pf6N2E2_2359 0.63 +1.0 2,794,112 + +0.8 2,794,115 + Pf6N2E2_2359 0.79 +0.4 2,794,115 + Pf6N2E2_2359 0.79 +0.8 2,794,115 + Pf6N2E2_2359 0.79 +0.6 2,794,115 + Pf6N2E2_2359 0.79 +0.3 2,794,116 - Pf6N2E2_2359 0.79 +1.6 2,794,116 - Pf6N2E2_2359 0.79 -2.5 2,794,261 - -2.4 2,794,589 + +1.5 2,794,589 + +1.4 2,794,589 + +0.2 2,794,589 + -1.0 2,794,590 - -1.0 2,794,590 - +0.1 2,794,590 - -1.0 2,794,593 + -1.2 2,794,594 - -3.3 2,794,601 - +1.8 2,794,735 + +1.0 2,794,736 - -1.8 2,795,210 - Pf6N2E2_2360 0.35 -1.0 2,795,305 - Pf6N2E2_2360 0.41 +0.6 2,795,476 + -0.6 2,795,477 - Pf6N2E2_2360 0.51 -0.6 2,795,477 - Pf6N2E2_2360 0.51 -0.8 2,795,480 + Pf6N2E2_2360 0.51 +0.0 2,795,481 - Pf6N2E2_2360 0.51 -0.2 2,795,500 + Pf6N2E2_2360 0.52 -0.0 2,795,500 + Pf6N2E2_2360 0.52 -2.4 2,795,501 - Pf6N2E2_2360 0.52 -2.9 2,795,717 + Pf6N2E2_2360 0.64 +1.6 2,795,718 - Pf6N2E2_2360 0.64 -2.3 2,795,718 - Pf6N2E2_2360 0.64 -3.1 2,795,718 - Pf6N2E2_2360 0.64 +0.3 2,796,188 + -0.2 2,796,221 - +0.3 2,796,268 + -0.3 2,796,269 - -1.0 2,796,269 - -1.7 2,796,369 + -1.8 2,796,369 + -2.2 2,796,369 + +0.8 2,796,369 + -1.2 2,796,369 + +1.0 2,796,369 + +1.4 2,796,370 - +2.7 2,796,370 - +1.3 2,796,370 - +0.3 2,796,370 - -2.3 2,796,370 - -1.2 2,796,370 - -1.3 2,796,429 + -1.6 2,796,430 - -0.2 2,796,430 - +0.8 2,796,430 - -1.2 2,796,430 - -1.6 2,796,480 + Pf6N2E2_2361 0.20 +0.9 2,796,481 - Pf6N2E2_2361 0.20 +0.8 2,796,481 - Pf6N2E2_2361 0.20 +0.8 2,796,481 - Pf6N2E2_2361 0.20 +2.2 2,796,540 + Pf6N2E2_2361 0.33 +1.7 2,796,541 - Pf6N2E2_2361 0.33 +0.5 2,796,589 - Pf6N2E2_2361 0.43 +1.9 2,796,589 - Pf6N2E2_2361 0.43 +0.9 2,796,651 + Pf6N2E2_2361 0.55 -1.6 2,796,663 + Pf6N2E2_2361 0.58 +1.4 2,796,663 + Pf6N2E2_2361 0.58 -0.6 2,796,663 + Pf6N2E2_2361 0.58 -1.6 2,796,663 + Pf6N2E2_2361 0.58 +2.0 2,796,664 - Pf6N2E2_2361 0.58 +0.1 2,796,673 - Pf6N2E2_2361 0.60 +1.4 2,796,726 + Pf6N2E2_2361 0.71 -0.5 2,796,726 + Pf6N2E2_2361 0.71 +2.3 2,796,727 - Pf6N2E2_2361 0.71 +0.6 2,796,727 - Pf6N2E2_2361 0.71 -1.0 2,796,727 - Pf6N2E2_2361 0.71 +1.6 2,796,727 - Pf6N2E2_2361 0.71 -2.3 2,796,747 + Pf6N2E2_2361 0.75 +0.5 2,797,249 + -2.2
Or see this region's nucleotide sequence