Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_227 and Pf6N2E2_228 are separated by 21 nucleotides Pf6N2E2_228 and Pf6N2E2_229 are separated by 2 nucleotides
Pf6N2E2_227 - 3-(3-hydroxy-phenyl)propionate hydroxylase (EC 1.14.13.-), at 288,729 to 290,375
_227
Pf6N2E2_228 - Transcriptional regulator, IclR family, at 290,397 to 291,218
_228
Pf6N2E2_229 - Putative fumarylacetoacetate (FAA) hydrolase, at 291,221 to 292,072
_229
Position (kb) 290
291
292 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 289.489 kb on + strand, within Pf6N2E2_227 at 289.489 kb on + strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227 at 289.574 kb on - strand, within Pf6N2E2_227 at 289.750 kb on + strand, within Pf6N2E2_227 at 289.750 kb on + strand, within Pf6N2E2_227 at 289.888 kb on + strand, within Pf6N2E2_227 at 289.888 kb on + strand, within Pf6N2E2_227 at 289.889 kb on - strand, within Pf6N2E2_227 at 289.941 kb on + strand, within Pf6N2E2_227 at 289.942 kb on - strand, within Pf6N2E2_227 at 290.078 kb on - strand, within Pf6N2E2_227 at 290.134 kb on + strand, within Pf6N2E2_227 at 290.134 kb on + strand, within Pf6N2E2_227 at 290.134 kb on + strand, within Pf6N2E2_227 at 290.134 kb on + strand, within Pf6N2E2_227 at 290.135 kb on - strand, within Pf6N2E2_227 at 290.135 kb on - strand, within Pf6N2E2_227 at 290.135 kb on - strand, within Pf6N2E2_227 at 290.135 kb on - strand at 290.379 kb on + strand at 290.379 kb on + strand at 290.582 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.618 kb on - strand, within Pf6N2E2_228 at 290.801 kb on - strand, within Pf6N2E2_228 at 290.970 kb on - strand, within Pf6N2E2_228 at 290.993 kb on - strand, within Pf6N2E2_228 at 290.993 kb on - strand, within Pf6N2E2_228 at 291.208 kb on - strand at 291.208 kb on - strand at 291.208 kb on - strand at 291.208 kb on - strand at 291.208 kb on - strand at 291.208 kb on - strand at 291.208 kb on - strand at 291.223 kb on - strand at 291.607 kb on - strand, within Pf6N2E2_229 at 291.607 kb on - strand, within Pf6N2E2_229 at 291.607 kb on - strand, within Pf6N2E2_229 at 291.784 kb on - strand, within Pf6N2E2_229 at 291.826 kb on - strand, within Pf6N2E2_229 at 292.032 kb on + strand at 292.032 kb on + strand at 292.032 kb on + strand at 292.032 kb on + strand at 292.033 kb on - strand at 292.036 kb on + strand at 292.037 kb on - strand at 292.037 kb on - strand at 292.039 kb on + strand at 292.055 kb on - strand at 292.055 kb on - strand at 292.110 kb on - strand at 292.196 kb on - strand at 292.196 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 289,489 + Pf6N2E2_227 0.46 +0.9 289,489 + Pf6N2E2_227 0.46 -1.8 289,574 - Pf6N2E2_227 0.51 -2.4 289,574 - Pf6N2E2_227 0.51 -0.0 289,574 - Pf6N2E2_227 0.51 +0.4 289,574 - Pf6N2E2_227 0.51 -0.1 289,750 + Pf6N2E2_227 0.62 -2.1 289,750 + Pf6N2E2_227 0.62 -1.6 289,888 + Pf6N2E2_227 0.70 -2.9 289,888 + Pf6N2E2_227 0.70 +0.1 289,889 - Pf6N2E2_227 0.70 +0.1 289,941 + Pf6N2E2_227 0.74 +0.1 289,942 - Pf6N2E2_227 0.74 -2.1 290,078 - Pf6N2E2_227 0.82 +1.5 290,134 + Pf6N2E2_227 0.85 -1.8 290,134 + Pf6N2E2_227 0.85 -0.3 290,134 + Pf6N2E2_227 0.85 -0.3 290,134 + Pf6N2E2_227 0.85 +1.1 290,135 - Pf6N2E2_227 0.85 -0.2 290,135 - Pf6N2E2_227 0.85 -0.6 290,135 - Pf6N2E2_227 0.85 +0.7 290,135 - -0.9 290,379 + -1.1 290,379 + +0.9 290,582 - Pf6N2E2_228 0.23 -0.6 290,618 - Pf6N2E2_228 0.27 +0.5 290,618 - Pf6N2E2_228 0.27 -0.3 290,618 - Pf6N2E2_228 0.27 -3.2 290,618 - Pf6N2E2_228 0.27 +2.8 290,618 - Pf6N2E2_228 0.27 +0.4 290,618 - Pf6N2E2_228 0.27 -1.9 290,801 - Pf6N2E2_228 0.49 -0.9 290,970 - Pf6N2E2_228 0.70 -1.6 290,993 - Pf6N2E2_228 0.73 -1.8 290,993 - Pf6N2E2_228 0.73 +0.2 291,208 - +1.1 291,208 - -2.7 291,208 - +0.9 291,208 - -0.0 291,208 - -1.8 291,208 - -2.0 291,208 - +2.7 291,223 - -0.5 291,607 - Pf6N2E2_229 0.45 -0.1 291,607 - Pf6N2E2_229 0.45 -0.5 291,607 - Pf6N2E2_229 0.45 -2.2 291,784 - Pf6N2E2_229 0.66 +0.8 291,826 - Pf6N2E2_229 0.71 +0.7 292,032 + -1.0 292,032 + -1.9 292,032 + -2.0 292,032 + +1.7 292,033 - +2.4 292,036 + -1.4 292,037 - -3.2 292,037 - -0.6 292,039 + -0.8 292,055 - +1.7 292,055 - -0.1 292,110 - +1.7 292,196 - +1.3 292,196 - +2.2
Or see this region's nucleotide sequence