Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2275 and Pf6N2E2_2276 are separated by 130 nucleotides Pf6N2E2_2276 and Pf6N2E2_2277 are separated by 99 nucleotides
Pf6N2E2_2275 - Thiamin biosynthesis lipoprotein ApbE, at 2,689,578 to 2,690,567
_2275
Pf6N2E2_2276 - Permeases of the major facilitator superfamily, at 2,690,698 to 2,691,996
_2276
Pf6N2E2_2277 - NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13), at 2,692,096 to 2,693,481
_2277
Position (kb)
2690
2691
2692 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2689.788 kb on + strand, within Pf6N2E2_2275 at 2689.788 kb on + strand, within Pf6N2E2_2275 at 2689.788 kb on + strand, within Pf6N2E2_2275 at 2689.789 kb on - strand, within Pf6N2E2_2275 at 2689.789 kb on - strand, within Pf6N2E2_2275 at 2689.852 kb on + strand, within Pf6N2E2_2275 at 2689.853 kb on - strand, within Pf6N2E2_2275 at 2689.856 kb on + strand, within Pf6N2E2_2275 at 2689.856 kb on + strand, within Pf6N2E2_2275 at 2689.856 kb on + strand, within Pf6N2E2_2275 at 2689.857 kb on - strand, within Pf6N2E2_2275 at 2690.014 kb on + strand, within Pf6N2E2_2275 at 2690.014 kb on + strand, within Pf6N2E2_2275 at 2690.015 kb on - strand, within Pf6N2E2_2275 at 2690.015 kb on - strand, within Pf6N2E2_2275 at 2690.191 kb on - strand, within Pf6N2E2_2275 at 2690.336 kb on + strand, within Pf6N2E2_2275 at 2690.336 kb on + strand, within Pf6N2E2_2275 at 2690.336 kb on + strand, within Pf6N2E2_2275 at 2690.337 kb on - strand, within Pf6N2E2_2275 at 2690.337 kb on - strand, within Pf6N2E2_2275 at 2690.337 kb on - strand, within Pf6N2E2_2275 at 2690.337 kb on - strand, within Pf6N2E2_2275 at 2690.337 kb on - strand, within Pf6N2E2_2275 at 2690.337 kb on - strand, within Pf6N2E2_2275 at 2690.337 kb on - strand, within Pf6N2E2_2275 at 2690.383 kb on + strand, within Pf6N2E2_2275 at 2690.474 kb on - strand at 2690.474 kb on - strand at 2690.486 kb on - strand at 2690.589 kb on + strand at 2690.589 kb on + strand at 2690.629 kb on - strand at 2690.668 kb on + strand at 2690.668 kb on + strand at 2690.669 kb on - strand at 2690.689 kb on + strand at 2690.690 kb on - strand at 2690.690 kb on - strand at 2690.693 kb on + strand at 2690.693 kb on + strand at 2690.693 kb on + strand at 2690.693 kb on + strand at 2690.693 kb on + strand at 2690.693 kb on + strand at 2690.694 kb on - strand at 2690.694 kb on - strand at 2690.694 kb on - strand at 2690.694 kb on - strand at 2690.694 kb on - strand at 2690.694 kb on - strand at 2690.694 kb on - strand at 2690.694 kb on - strand at 2690.694 kb on - strand at 2690.694 kb on - strand at 2690.694 kb on - strand at 2690.694 kb on - strand at 2690.779 kb on + strand at 2690.779 kb on + strand at 2690.780 kb on - strand at 2690.780 kb on - strand at 2690.914 kb on + strand, within Pf6N2E2_2276 at 2691.101 kb on - strand, within Pf6N2E2_2276 at 2691.101 kb on - strand, within Pf6N2E2_2276 at 2691.443 kb on - strand, within Pf6N2E2_2276 at 2691.457 kb on + strand, within Pf6N2E2_2276 at 2691.458 kb on - strand, within Pf6N2E2_2276 at 2691.588 kb on + strand, within Pf6N2E2_2276 at 2691.589 kb on - strand, within Pf6N2E2_2276 at 2691.721 kb on + strand, within Pf6N2E2_2276 at 2691.721 kb on + strand, within Pf6N2E2_2276 at 2691.721 kb on + strand, within Pf6N2E2_2276 at 2691.796 kb on + strand, within Pf6N2E2_2276 at 2691.797 kb on - strand, within Pf6N2E2_2276 at 2691.797 kb on - strand, within Pf6N2E2_2276 at 2691.828 kb on + strand, within Pf6N2E2_2276 at 2691.829 kb on - strand, within Pf6N2E2_2276 at 2691.829 kb on - strand, within Pf6N2E2_2276 at 2691.843 kb on + strand, within Pf6N2E2_2276 at 2691.843 kb on + strand, within Pf6N2E2_2276 at 2691.843 kb on + strand, within Pf6N2E2_2276 at 2691.843 kb on + strand, within Pf6N2E2_2276 at 2691.843 kb on + strand, within Pf6N2E2_2276 at 2691.843 kb on + strand, within Pf6N2E2_2276 at 2691.843 kb on + strand, within Pf6N2E2_2276 at 2691.847 kb on + strand, within Pf6N2E2_2276 at 2691.847 kb on + strand, within Pf6N2E2_2276 at 2691.847 kb on + strand, within Pf6N2E2_2276 at 2691.847 kb on + strand, within Pf6N2E2_2276 at 2691.847 kb on + strand, within Pf6N2E2_2276 at 2691.848 kb on - strand, within Pf6N2E2_2276 at 2691.848 kb on - strand, within Pf6N2E2_2276 at 2691.848 kb on - strand, within Pf6N2E2_2276 at 2691.848 kb on - strand, within Pf6N2E2_2276 at 2691.848 kb on - strand, within Pf6N2E2_2276 at 2691.848 kb on - strand, within Pf6N2E2_2276 at 2691.997 kb on + strand at 2691.997 kb on + strand at 2692.049 kb on - strand at 2692.049 kb on - strand at 2692.121 kb on - strand at 2692.191 kb on - strand at 2692.191 kb on - strand at 2692.343 kb on + strand, within Pf6N2E2_2277 at 2692.580 kb on + strand, within Pf6N2E2_2277 at 2692.865 kb on + strand, within Pf6N2E2_2277 at 2692.874 kb on + strand, within Pf6N2E2_2277 at 2692.874 kb on + strand, within Pf6N2E2_2277
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 2,689,788 + Pf6N2E2_2275 0.21 +0.0 2,689,788 + Pf6N2E2_2275 0.21 -1.7 2,689,788 + Pf6N2E2_2275 0.21 +0.5 2,689,789 - Pf6N2E2_2275 0.21 +0.1 2,689,789 - Pf6N2E2_2275 0.21 -1.3 2,689,852 + Pf6N2E2_2275 0.28 -0.3 2,689,853 - Pf6N2E2_2275 0.28 -0.5 2,689,856 + Pf6N2E2_2275 0.28 -2.0 2,689,856 + Pf6N2E2_2275 0.28 +0.7 2,689,856 + Pf6N2E2_2275 0.28 -2.7 2,689,857 - Pf6N2E2_2275 0.28 +0.3 2,690,014 + Pf6N2E2_2275 0.44 -0.6 2,690,014 + Pf6N2E2_2275 0.44 +1.5 2,690,015 - Pf6N2E2_2275 0.44 +0.7 2,690,015 - Pf6N2E2_2275 0.44 +0.7 2,690,191 - Pf6N2E2_2275 0.62 -1.9 2,690,336 + Pf6N2E2_2275 0.77 -0.9 2,690,336 + Pf6N2E2_2275 0.77 +0.7 2,690,336 + Pf6N2E2_2275 0.77 -0.4 2,690,337 - Pf6N2E2_2275 0.77 +0.3 2,690,337 - Pf6N2E2_2275 0.77 +1.2 2,690,337 - Pf6N2E2_2275 0.77 -1.8 2,690,337 - Pf6N2E2_2275 0.77 -0.2 2,690,337 - Pf6N2E2_2275 0.77 +0.5 2,690,337 - Pf6N2E2_2275 0.77 +1.2 2,690,337 - Pf6N2E2_2275 0.77 +0.2 2,690,383 + Pf6N2E2_2275 0.81 +0.3 2,690,474 - +1.8 2,690,474 - -0.8 2,690,486 - +0.7 2,690,589 + -1.4 2,690,589 + +2.2 2,690,629 - -2.3 2,690,668 + -0.8 2,690,668 + -2.7 2,690,669 - +1.4 2,690,689 + -1.6 2,690,690 - -1.3 2,690,690 - +0.1 2,690,693 + -2.1 2,690,693 + +0.2 2,690,693 + -0.5 2,690,693 + +0.7 2,690,693 + -0.6 2,690,693 + -1.0 2,690,694 - +1.5 2,690,694 - +0.9 2,690,694 - -1.6 2,690,694 - -2.3 2,690,694 - +0.7 2,690,694 - -2.1 2,690,694 - +1.2 2,690,694 - +1.1 2,690,694 - -0.1 2,690,694 - -0.5 2,690,694 - -0.8 2,690,694 - -2.9 2,690,779 + +1.7 2,690,779 + +0.2 2,690,780 - +0.7 2,690,780 - +0.9 2,690,914 + Pf6N2E2_2276 0.17 -0.3 2,691,101 - Pf6N2E2_2276 0.31 +0.0 2,691,101 - Pf6N2E2_2276 0.31 +1.0 2,691,443 - Pf6N2E2_2276 0.57 -2.3 2,691,457 + Pf6N2E2_2276 0.58 +0.9 2,691,458 - Pf6N2E2_2276 0.59 +0.7 2,691,588 + Pf6N2E2_2276 0.69 -1.2 2,691,589 - Pf6N2E2_2276 0.69 -0.8 2,691,721 + Pf6N2E2_2276 0.79 +0.7 2,691,721 + Pf6N2E2_2276 0.79 -0.4 2,691,721 + Pf6N2E2_2276 0.79 -1.0 2,691,796 + Pf6N2E2_2276 0.85 -1.8 2,691,797 - Pf6N2E2_2276 0.85 -0.1 2,691,797 - Pf6N2E2_2276 0.85 -0.7 2,691,828 + Pf6N2E2_2276 0.87 -3.1 2,691,829 - Pf6N2E2_2276 0.87 -3.1 2,691,829 - Pf6N2E2_2276 0.87 -0.6 2,691,843 + Pf6N2E2_2276 0.88 -2.1 2,691,843 + Pf6N2E2_2276 0.88 -0.3 2,691,843 + Pf6N2E2_2276 0.88 +2.2 2,691,843 + Pf6N2E2_2276 0.88 -1.1 2,691,843 + Pf6N2E2_2276 0.88 +1.0 2,691,843 + Pf6N2E2_2276 0.88 -1.2 2,691,843 + Pf6N2E2_2276 0.88 -1.6 2,691,847 + Pf6N2E2_2276 0.88 +1.0 2,691,847 + Pf6N2E2_2276 0.88 -3.0 2,691,847 + Pf6N2E2_2276 0.88 +0.7 2,691,847 + Pf6N2E2_2276 0.88 -0.2 2,691,847 + Pf6N2E2_2276 0.88 +0.9 2,691,848 - Pf6N2E2_2276 0.89 +1.0 2,691,848 - Pf6N2E2_2276 0.89 -0.2 2,691,848 - Pf6N2E2_2276 0.89 -1.4 2,691,848 - Pf6N2E2_2276 0.89 +0.2 2,691,848 - Pf6N2E2_2276 0.89 +1.6 2,691,848 - Pf6N2E2_2276 0.89 -0.8 2,691,997 + +0.6 2,691,997 + +0.9 2,692,049 - +1.5 2,692,049 - -1.7 2,692,121 - -1.8 2,692,191 - +2.3 2,692,191 - +2.2 2,692,343 + Pf6N2E2_2277 0.18 +2.4 2,692,580 + Pf6N2E2_2277 0.35 +5.3 2,692,865 + Pf6N2E2_2277 0.55 -2.1 2,692,874 + Pf6N2E2_2277 0.56 +1.0 2,692,874 + Pf6N2E2_2277 0.56 +3.1
Or see this region's nucleotide sequence