Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_201 and Pf6N2E2_202 overlap by 4 nucleotides Pf6N2E2_202 and Pf6N2E2_203 overlap by 11 nucleotides
Pf6N2E2_201 - UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14), at 251,958 to 253,067
_201
Pf6N2E2_202 - 4Fe-4S ferredoxin, iron-sulfur binding, at 253,064 to 254,650
_202
Pf6N2E2_203 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 254,640 to 255,920
_203
Position (kb) 253
254
255 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 252.241 kb on + strand, within Pf6N2E2_201 at 252.242 kb on - strand, within Pf6N2E2_201 at 252.242 kb on - strand, within Pf6N2E2_201 at 252.242 kb on - strand, within Pf6N2E2_201 at 252.274 kb on + strand, within Pf6N2E2_201 at 252.274 kb on + strand, within Pf6N2E2_201 at 252.274 kb on + strand, within Pf6N2E2_201 at 252.688 kb on - strand, within Pf6N2E2_201 at 252.688 kb on - strand, within Pf6N2E2_201 at 253.053 kb on + strand at 253.054 kb on - strand at 253.054 kb on - strand at 253.054 kb on - strand at 253.091 kb on + strand at 253.091 kb on + strand at 253.091 kb on + strand at 253.183 kb on + strand at 253.183 kb on + strand at 253.183 kb on + strand at 253.184 kb on - strand at 253.338 kb on + strand, within Pf6N2E2_202 at 253.338 kb on + strand, within Pf6N2E2_202 at 253.339 kb on - strand, within Pf6N2E2_202 at 253.472 kb on - strand, within Pf6N2E2_202 at 253.472 kb on - strand, within Pf6N2E2_202 at 253.472 kb on - strand, within Pf6N2E2_202 at 253.604 kb on - strand, within Pf6N2E2_202 at 253.941 kb on + strand, within Pf6N2E2_202 at 253.941 kb on + strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 253.942 kb on - strand, within Pf6N2E2_202 at 254.157 kb on + strand, within Pf6N2E2_202 at 254.157 kb on + strand, within Pf6N2E2_202 at 254.157 kb on + strand, within Pf6N2E2_202 at 254.157 kb on + strand, within Pf6N2E2_202 at 254.158 kb on - strand, within Pf6N2E2_202 at 254.158 kb on - strand, within Pf6N2E2_202 at 254.158 kb on - strand, within Pf6N2E2_202 at 254.218 kb on - strand, within Pf6N2E2_202 at 254.218 kb on - strand, within Pf6N2E2_202 at 254.239 kb on - strand, within Pf6N2E2_202 at 254.826 kb on + strand, within Pf6N2E2_203 at 254.960 kb on + strand, within Pf6N2E2_203 at 255.029 kb on - strand, within Pf6N2E2_203 at 255.232 kb on - strand, within Pf6N2E2_203 at 255.384 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.416 kb on + strand, within Pf6N2E2_203 at 255.417 kb on - strand, within Pf6N2E2_203 at 255.514 kb on + strand, within Pf6N2E2_203 at 255.514 kb on + strand, within Pf6N2E2_203 at 255.514 kb on + strand, within Pf6N2E2_203 at 255.515 kb on - strand, within Pf6N2E2_203 at 255.515 kb on - strand, within Pf6N2E2_203 at 255.515 kb on - strand, within Pf6N2E2_203
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 252,241 + Pf6N2E2_201 0.25 -1.5 252,242 - Pf6N2E2_201 0.26 -1.1 252,242 - Pf6N2E2_201 0.26 -0.8 252,242 - Pf6N2E2_201 0.26 +0.6 252,274 + Pf6N2E2_201 0.28 +1.1 252,274 + Pf6N2E2_201 0.28 -2.6 252,274 + Pf6N2E2_201 0.28 +1.4 252,688 - Pf6N2E2_201 0.66 +2.0 252,688 - Pf6N2E2_201 0.66 +0.1 253,053 + +1.4 253,054 - +1.7 253,054 - -3.4 253,054 - -0.9 253,091 + +1.4 253,091 + -2.7 253,091 + +1.7 253,183 + -2.0 253,183 + +1.8 253,183 + -0.1 253,184 - -1.6 253,338 + Pf6N2E2_202 0.17 +0.1 253,338 + Pf6N2E2_202 0.17 +1.2 253,339 - Pf6N2E2_202 0.17 +1.1 253,472 - Pf6N2E2_202 0.26 -1.8 253,472 - Pf6N2E2_202 0.26 +2.6 253,472 - Pf6N2E2_202 0.26 -0.6 253,604 - Pf6N2E2_202 0.34 +2.6 253,941 + Pf6N2E2_202 0.55 +2.0 253,941 + Pf6N2E2_202 0.55 +3.6 253,942 - Pf6N2E2_202 0.55 +2.6 253,942 - Pf6N2E2_202 0.55 -2.1 253,942 - Pf6N2E2_202 0.55 +0.7 253,942 - Pf6N2E2_202 0.55 +0.1 253,942 - Pf6N2E2_202 0.55 +0.4 253,942 - Pf6N2E2_202 0.55 -2.0 253,942 - Pf6N2E2_202 0.55 -0.9 253,942 - Pf6N2E2_202 0.55 -0.3 253,942 - Pf6N2E2_202 0.55 -1.9 253,942 - Pf6N2E2_202 0.55 +1.1 254,157 + Pf6N2E2_202 0.69 +1.0 254,157 + Pf6N2E2_202 0.69 -0.5 254,157 + Pf6N2E2_202 0.69 -1.3 254,157 + Pf6N2E2_202 0.69 -2.2 254,158 - Pf6N2E2_202 0.69 +0.5 254,158 - Pf6N2E2_202 0.69 +2.9 254,158 - Pf6N2E2_202 0.69 +0.5 254,218 - Pf6N2E2_202 0.73 +1.1 254,218 - Pf6N2E2_202 0.73 +1.5 254,239 - Pf6N2E2_202 0.74 +0.5 254,826 + Pf6N2E2_203 0.15 +1.5 254,960 + Pf6N2E2_203 0.25 -0.9 255,029 - Pf6N2E2_203 0.30 +0.2 255,232 - Pf6N2E2_203 0.46 -0.4 255,384 + Pf6N2E2_203 0.58 -0.3 255,416 + Pf6N2E2_203 0.61 -0.2 255,416 + Pf6N2E2_203 0.61 -1.1 255,416 + Pf6N2E2_203 0.61 +0.3 255,416 + Pf6N2E2_203 0.61 +1.6 255,416 + Pf6N2E2_203 0.61 +0.8 255,416 + Pf6N2E2_203 0.61 +1.6 255,417 - Pf6N2E2_203 0.61 +1.7 255,514 + Pf6N2E2_203 0.68 +0.3 255,514 + Pf6N2E2_203 0.68 -0.1 255,514 + Pf6N2E2_203 0.68 +1.4 255,515 - Pf6N2E2_203 0.68 -1.8 255,515 - Pf6N2E2_203 0.68 +0.3 255,515 - Pf6N2E2_203 0.68 +1.2
Or see this region's nucleotide sequence