Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1943 and Pf6N2E2_1944 are separated by 72 nucleotides Pf6N2E2_1944 and Pf6N2E2_1945 are separated by 11 nucleotides Pf6N2E2_1945 and Pf6N2E2_1946 overlap by 4 nucleotides Pf6N2E2_1946 and Pf6N2E2_1947 overlap by 29 nucleotides
Pf6N2E2_1943 - Coenzyme PQQ synthesis protein A, at 2,305,458 to 2,305,598
_1943
Pf6N2E2_1944 - Coenzyme PQQ synthesis protein B, at 2,305,671 to 2,306,582
_1944
Pf6N2E2_1945 - Pyrroloquinoline-quinone synthase (EC 1.3.3.11), at 2,306,594 to 2,307,346
_1945
Pf6N2E2_1946 - Coenzyme PQQ synthesis protein D, at 2,307,343 to 2,307,618
_1946
Pf6N2E2_1947 - Coenzyme PQQ synthesis protein E, at 2,307,590 to 2,308,759
_1947
Position (kb)
2306
2307
2308 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2305.646 kb on + strand at 2305.647 kb on - strand at 2305.687 kb on + strand at 2305.688 kb on - strand at 2305.688 kb on - strand at 2305.688 kb on - strand at 2306.556 kb on + strand at 2306.570 kb on + strand at 2306.570 kb on + strand at 2306.580 kb on + strand at 2306.580 kb on + strand at 2306.580 kb on + strand at 2306.654 kb on + strand at 2306.657 kb on + strand at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.690 kb on + strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.691 kb on - strand, within Pf6N2E2_1945 at 2306.748 kb on - strand, within Pf6N2E2_1945 at 2306.941 kb on - strand, within Pf6N2E2_1945 at 2306.972 kb on + strand at 2307.113 kb on + strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.114 kb on - strand, within Pf6N2E2_1945 at 2307.169 kb on + strand, within Pf6N2E2_1945 at 2307.182 kb on + strand, within Pf6N2E2_1945 at 2307.183 kb on - strand, within Pf6N2E2_1945 at 2307.183 kb on - strand, within Pf6N2E2_1945 at 2307.183 kb on - strand, within Pf6N2E2_1945 at 2307.183 kb on - strand, within Pf6N2E2_1945 at 2307.183 kb on - strand, within Pf6N2E2_1945 at 2307.296 kb on + strand at 2307.337 kb on + strand at 2307.337 kb on + strand at 2307.338 kb on - strand at 2307.338 kb on - strand at 2307.343 kb on - strand at 2307.343 kb on - strand at 2307.683 kb on + strand at 2307.707 kb on + strand, within Pf6N2E2_1947 at 2307.708 kb on - strand, within Pf6N2E2_1947 at 2307.887 kb on + strand, within Pf6N2E2_1947 at 2307.887 kb on + strand, within Pf6N2E2_1947 at 2307.887 kb on + strand, within Pf6N2E2_1947 at 2307.887 kb on + strand, within Pf6N2E2_1947 at 2307.888 kb on - strand, within Pf6N2E2_1947 at 2307.888 kb on - strand, within Pf6N2E2_1947 at 2308.248 kb on + strand, within Pf6N2E2_1947
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 2,305,646 + +1.7 2,305,647 - -0.3 2,305,687 + +0.7 2,305,688 - +0.8 2,305,688 - +1.2 2,305,688 - -2.6 2,306,556 + -0.6 2,306,570 + -2.0 2,306,570 + -2.6 2,306,580 + +2.0 2,306,580 + +1.2 2,306,580 + -0.9 2,306,654 + -2.1 2,306,657 + -1.9 2,306,690 + Pf6N2E2_1945 0.13 -2.8 2,306,690 + Pf6N2E2_1945 0.13 -1.1 2,306,690 + Pf6N2E2_1945 0.13 +0.9 2,306,690 + Pf6N2E2_1945 0.13 -1.1 2,306,690 + Pf6N2E2_1945 0.13 +1.1 2,306,690 + Pf6N2E2_1945 0.13 +2.8 2,306,691 - Pf6N2E2_1945 0.13 -1.3 2,306,691 - Pf6N2E2_1945 0.13 +3.4 2,306,691 - Pf6N2E2_1945 0.13 +1.0 2,306,691 - Pf6N2E2_1945 0.13 +2.1 2,306,691 - Pf6N2E2_1945 0.13 +1.2 2,306,691 - Pf6N2E2_1945 0.13 -2.9 2,306,691 - Pf6N2E2_1945 0.13 +1.5 2,306,691 - Pf6N2E2_1945 0.13 -0.6 2,306,691 - Pf6N2E2_1945 0.13 +2.1 2,306,748 - Pf6N2E2_1945 0.20 -2.5 2,306,941 - Pf6N2E2_1945 0.46 -2.5 2,306,972 + +0.7 2,307,113 + Pf6N2E2_1945 0.69 +3.4 2,307,114 - Pf6N2E2_1945 0.69 -1.0 2,307,114 - Pf6N2E2_1945 0.69 +2.4 2,307,114 - Pf6N2E2_1945 0.69 -1.1 2,307,114 - Pf6N2E2_1945 0.69 -0.4 2,307,114 - Pf6N2E2_1945 0.69 -1.1 2,307,114 - Pf6N2E2_1945 0.69 +0.4 2,307,169 + Pf6N2E2_1945 0.76 -0.1 2,307,182 + Pf6N2E2_1945 0.78 -0.4 2,307,183 - Pf6N2E2_1945 0.78 -0.0 2,307,183 - Pf6N2E2_1945 0.78 +1.8 2,307,183 - Pf6N2E2_1945 0.78 -0.9 2,307,183 - Pf6N2E2_1945 0.78 -1.6 2,307,183 - Pf6N2E2_1945 0.78 +0.5 2,307,296 + +1.1 2,307,337 + -0.8 2,307,337 + -0.0 2,307,338 - -0.3 2,307,338 - +1.2 2,307,343 - +0.7 2,307,343 - -0.2 2,307,683 + +1.2 2,307,707 + Pf6N2E2_1947 0.10 -2.2 2,307,708 - Pf6N2E2_1947 0.10 -0.3 2,307,887 + Pf6N2E2_1947 0.25 -1.5 2,307,887 + Pf6N2E2_1947 0.25 +0.9 2,307,887 + Pf6N2E2_1947 0.25 -0.3 2,307,887 + Pf6N2E2_1947 0.25 -1.9 2,307,888 - Pf6N2E2_1947 0.25 -1.9 2,307,888 - Pf6N2E2_1947 0.25 +0.7 2,308,248 + Pf6N2E2_1947 0.56 -1.8
Or see this region's nucleotide sequence