Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1662 and Pf6N2E2_1663 are separated by 31 nucleotides Pf6N2E2_1663 and Pf6N2E2_1664 overlap by 4 nucleotides
Pf6N2E2_1662 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase., at 1,968,970 to 1,969,818
_1662
Pf6N2E2_1663 - 2-keto-3-deoxy-L-fuconate dehydrogenase, at 1,969,850 to 1,970,590
_1663
Pf6N2E2_1664 - Nitrate/nitrite transporter, at 1,970,587 to 1,971,969
_1664
Position (kb)
1969
1970
1971 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1969.222 kb on + strand, within Pf6N2E2_1662 at 1969.223 kb on - strand, within Pf6N2E2_1662 at 1969.223 kb on - strand, within Pf6N2E2_1662 at 1969.223 kb on - strand, within Pf6N2E2_1662 at 1969.409 kb on + strand, within Pf6N2E2_1662 at 1969.410 kb on - strand, within Pf6N2E2_1662 at 1969.410 kb on - strand, within Pf6N2E2_1662 at 1969.521 kb on + strand, within Pf6N2E2_1662 at 1969.522 kb on - strand, within Pf6N2E2_1662 at 1969.810 kb on + strand at 1969.810 kb on + strand at 1969.855 kb on + strand at 1969.921 kb on - strand at 1969.921 kb on - strand at 1969.993 kb on - strand, within Pf6N2E2_1663 at 1970.274 kb on + strand, within Pf6N2E2_1663 at 1970.274 kb on + strand, within Pf6N2E2_1663 at 1970.333 kb on + strand, within Pf6N2E2_1663 at 1970.333 kb on + strand, within Pf6N2E2_1663 at 1970.333 kb on + strand, within Pf6N2E2_1663 at 1970.334 kb on - strand, within Pf6N2E2_1663 at 1970.334 kb on - strand, within Pf6N2E2_1663 at 1970.334 kb on - strand, within Pf6N2E2_1663 at 1970.353 kb on - strand, within Pf6N2E2_1663 at 1970.353 kb on - strand, within Pf6N2E2_1663 at 1970.567 kb on + strand at 1970.582 kb on + strand at 1970.583 kb on - strand at 1970.699 kb on - strand at 1970.699 kb on - strand at 1970.699 kb on - strand at 1970.699 kb on - strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.702 kb on + strand at 1970.703 kb on - strand at 1970.703 kb on - strand at 1970.703 kb on - strand at 1971.137 kb on + strand, within Pf6N2E2_1664 at 1971.427 kb on + strand, within Pf6N2E2_1664 at 1971.509 kb on + strand, within Pf6N2E2_1664 at 1971.510 kb on - strand, within Pf6N2E2_1664 at 1971.518 kb on + strand, within Pf6N2E2_1664 at 1971.519 kb on - strand, within Pf6N2E2_1664 at 1971.519 kb on - strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.579 kb on - strand, within Pf6N2E2_1664 at 1971.579 kb on - strand, within Pf6N2E2_1664 at 1971.579 kb on - strand, within Pf6N2E2_1664
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 1,969,222 + Pf6N2E2_1662 0.30 -3.3 1,969,223 - Pf6N2E2_1662 0.30 +0.5 1,969,223 - Pf6N2E2_1662 0.30 +0.6 1,969,223 - Pf6N2E2_1662 0.30 -0.5 1,969,409 + Pf6N2E2_1662 0.52 -0.8 1,969,410 - Pf6N2E2_1662 0.52 -1.5 1,969,410 - Pf6N2E2_1662 0.52 -0.2 1,969,521 + Pf6N2E2_1662 0.65 +0.6 1,969,522 - Pf6N2E2_1662 0.65 +0.9 1,969,810 + +1.3 1,969,810 + -0.6 1,969,855 + +1.7 1,969,921 - +0.8 1,969,921 - -1.9 1,969,993 - Pf6N2E2_1663 0.19 -0.9 1,970,274 + Pf6N2E2_1663 0.57 -2.2 1,970,274 + Pf6N2E2_1663 0.57 -0.1 1,970,333 + Pf6N2E2_1663 0.65 +1.8 1,970,333 + Pf6N2E2_1663 0.65 -2.6 1,970,333 + Pf6N2E2_1663 0.65 -3.1 1,970,334 - Pf6N2E2_1663 0.65 +0.8 1,970,334 - Pf6N2E2_1663 0.65 +0.9 1,970,334 - Pf6N2E2_1663 0.65 +0.9 1,970,353 - Pf6N2E2_1663 0.68 -1.3 1,970,353 - Pf6N2E2_1663 0.68 -1.3 1,970,567 + -1.7 1,970,582 + -0.3 1,970,583 - -3.3 1,970,699 - +1.3 1,970,699 - -0.9 1,970,699 - -0.5 1,970,699 - -0.5 1,970,702 + +2.2 1,970,702 + -0.1 1,970,702 + -0.6 1,970,702 + +0.7 1,970,702 + +0.7 1,970,702 + -1.9 1,970,702 + +0.4 1,970,702 + +0.3 1,970,702 + +2.3 1,970,703 - -1.4 1,970,703 - +1.7 1,970,703 - -0.5 1,971,137 + Pf6N2E2_1664 0.40 -2.0 1,971,427 + Pf6N2E2_1664 0.61 +1.7 1,971,509 + Pf6N2E2_1664 0.67 +1.1 1,971,510 - Pf6N2E2_1664 0.67 -0.1 1,971,518 + Pf6N2E2_1664 0.67 +0.7 1,971,519 - Pf6N2E2_1664 0.67 +0.4 1,971,519 - Pf6N2E2_1664 0.67 -1.0 1,971,578 + Pf6N2E2_1664 0.72 +1.3 1,971,578 + Pf6N2E2_1664 0.72 +0.7 1,971,578 + Pf6N2E2_1664 0.72 -1.1 1,971,578 + Pf6N2E2_1664 0.72 -0.3 1,971,579 - Pf6N2E2_1664 0.72 -1.3 1,971,579 - Pf6N2E2_1664 0.72 +0.5 1,971,579 - Pf6N2E2_1664 0.72 +0.7
Or see this region's nucleotide sequence