Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_140 and Pf6N2E2_141 are separated by 903 nucleotides Pf6N2E2_141 and Pf6N2E2_142 are separated by 192 nucleotides
Pf6N2E2_140 - Glutamate carboxypeptidase (EC 3.4.17.11), at 190,063 to 191,301
_140
Pf6N2E2_141 - Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1), at 192,205 to 193,539
_141
Pf6N2E2_142 - Exodeoxyribonuclease III (EC 3.1.11.2), at 193,732 to 194,544
_142
Position (kb) 192
193
194 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 191.227 kb on + strand at 191.227 kb on + strand at 191.228 kb on - strand at 191.260 kb on + strand at 191.307 kb on + strand at 191.307 kb on + strand at 191.308 kb on - strand at 191.458 kb on - strand at 191.464 kb on - strand at 191.468 kb on - strand at 191.468 kb on - strand at 191.468 kb on - strand at 191.817 kb on + strand at 191.817 kb on + strand at 191.818 kb on - strand at 191.818 kb on - strand at 191.818 kb on - strand at 191.958 kb on - strand at 191.958 kb on - strand at 191.958 kb on - strand at 191.964 kb on - strand at 192.317 kb on + strand at 192.449 kb on + strand, within Pf6N2E2_141 at 192.449 kb on + strand, within Pf6N2E2_141 at 192.450 kb on - strand, within Pf6N2E2_141 at 192.450 kb on - strand, within Pf6N2E2_141 at 192.450 kb on - strand, within Pf6N2E2_141 at 192.714 kb on - strand, within Pf6N2E2_141 at 192.827 kb on + strand, within Pf6N2E2_141 at 192.960 kb on + strand, within Pf6N2E2_141 at 192.960 kb on + strand, within Pf6N2E2_141 at 192.960 kb on + strand, within Pf6N2E2_141 at 192.960 kb on + strand, within Pf6N2E2_141 at 192.960 kb on + strand, within Pf6N2E2_141 at 192.965 kb on + strand, within Pf6N2E2_141 at 192.965 kb on + strand, within Pf6N2E2_141 at 192.965 kb on + strand, within Pf6N2E2_141 at 192.965 kb on + strand, within Pf6N2E2_141 at 192.965 kb on + strand, within Pf6N2E2_141 at 192.965 kb on + strand, within Pf6N2E2_141 at 192.965 kb on + strand, within Pf6N2E2_141 at 192.966 kb on - strand, within Pf6N2E2_141 at 192.966 kb on - strand, within Pf6N2E2_141 at 192.966 kb on - strand, within Pf6N2E2_141 at 192.966 kb on - strand, within Pf6N2E2_141 at 192.992 kb on + strand, within Pf6N2E2_141 at 192.992 kb on + strand, within Pf6N2E2_141 at 193.111 kb on - strand, within Pf6N2E2_141 at 193.428 kb on + strand at 193.448 kb on - strand at 193.577 kb on + strand at 193.577 kb on + strand at 193.620 kb on + strand at 193.628 kb on + strand at 193.628 kb on + strand at 193.629 kb on - strand at 193.629 kb on - strand at 194.216 kb on + strand, within Pf6N2E2_142 at 194.216 kb on + strand, within Pf6N2E2_142 at 194.315 kb on + strand, within Pf6N2E2_142 at 194.315 kb on + strand, within Pf6N2E2_142 at 194.316 kb on - strand, within Pf6N2E2_142 at 194.316 kb on - strand, within Pf6N2E2_142 at 194.316 kb on - strand, within Pf6N2E2_142 at 194.316 kb on - strand, within Pf6N2E2_142 at 194.378 kb on + strand, within Pf6N2E2_142
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 191,227 + -1.6 191,227 + +1.4 191,228 - +0.3 191,260 + -1.2 191,307 + +0.7 191,307 + +0.4 191,308 - -2.3 191,458 - -1.2 191,464 - -0.5 191,468 - +1.8 191,468 - -1.6 191,468 - +0.4 191,817 + +1.5 191,817 + -1.1 191,818 - +0.0 191,818 - -2.2 191,818 - -2.2 191,958 - +2.1 191,958 - -1.7 191,958 - -2.3 191,964 - -0.6 192,317 + +2.2 192,449 + Pf6N2E2_141 0.18 +0.0 192,449 + Pf6N2E2_141 0.18 -0.3 192,450 - Pf6N2E2_141 0.18 +1.1 192,450 - Pf6N2E2_141 0.18 -0.3 192,450 - Pf6N2E2_141 0.18 +1.9 192,714 - Pf6N2E2_141 0.38 +0.2 192,827 + Pf6N2E2_141 0.47 +1.2 192,960 + Pf6N2E2_141 0.57 +0.3 192,960 + Pf6N2E2_141 0.57 -1.5 192,960 + Pf6N2E2_141 0.57 +2.3 192,960 + Pf6N2E2_141 0.57 -1.4 192,960 + Pf6N2E2_141 0.57 -3.5 192,965 + Pf6N2E2_141 0.57 +0.0 192,965 + Pf6N2E2_141 0.57 +2.1 192,965 + Pf6N2E2_141 0.57 -1.6 192,965 + Pf6N2E2_141 0.57 +0.5 192,965 + Pf6N2E2_141 0.57 +0.7 192,965 + Pf6N2E2_141 0.57 +2.7 192,965 + Pf6N2E2_141 0.57 +1.0 192,966 - Pf6N2E2_141 0.57 +1.7 192,966 - Pf6N2E2_141 0.57 -0.8 192,966 - Pf6N2E2_141 0.57 +2.0 192,966 - Pf6N2E2_141 0.57 -0.1 192,992 + Pf6N2E2_141 0.59 -0.4 192,992 + Pf6N2E2_141 0.59 -1.1 193,111 - Pf6N2E2_141 0.68 +1.8 193,428 + +1.0 193,448 - -2.9 193,577 + -1.8 193,577 + +3.2 193,620 + +0.9 193,628 + -1.6 193,628 + -3.1 193,629 - +0.5 193,629 - -2.3 194,216 + Pf6N2E2_142 0.60 -2.0 194,216 + Pf6N2E2_142 0.60 +1.7 194,315 + Pf6N2E2_142 0.72 -2.2 194,315 + Pf6N2E2_142 0.72 +0.5 194,316 - Pf6N2E2_142 0.72 +0.7 194,316 - Pf6N2E2_142 0.72 -2.7 194,316 - Pf6N2E2_142 0.72 -2.8 194,316 - Pf6N2E2_142 0.72 -2.3 194,378 + Pf6N2E2_142 0.79 +0.6
Or see this region's nucleotide sequence