Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1182 and Pf6N2E2_1183 are separated by 152 nucleotides Pf6N2E2_1183 and Pf6N2E2_1184 are separated by 20 nucleotides
Pf6N2E2_1182 - Transcriptional regulator, LysR family, at 1,384,152 to 1,385,033
_1182
Pf6N2E2_1183 - Major facilitator family transporter, at 1,385,186 to 1,386,394
_1183
Pf6N2E2_1184 - L-Proline/Glycine betaine transporter ProP, at 1,386,415 to 1,387,719
_1184
Position (kb)
1385
1386
1387 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1384.241 kb on + strand, within Pf6N2E2_1182 at 1384.354 kb on + strand, within Pf6N2E2_1182 at 1384.354 kb on + strand, within Pf6N2E2_1182 at 1384.355 kb on - strand, within Pf6N2E2_1182 at 1384.466 kb on - strand, within Pf6N2E2_1182 at 1385.116 kb on - strand at 1385.126 kb on + strand at 1385.127 kb on - strand at 1385.127 kb on - strand at 1385.387 kb on + strand, within Pf6N2E2_1183 at 1385.388 kb on - strand, within Pf6N2E2_1183 at 1385.519 kb on + strand, within Pf6N2E2_1183 at 1385.519 kb on + strand, within Pf6N2E2_1183 at 1385.519 kb on + strand, within Pf6N2E2_1183 at 1385.520 kb on - strand at 1385.556 kb on + strand, within Pf6N2E2_1183 at 1385.769 kb on + strand, within Pf6N2E2_1183 at 1385.905 kb on + strand, within Pf6N2E2_1183 at 1385.909 kb on + strand, within Pf6N2E2_1183 at 1385.909 kb on + strand, within Pf6N2E2_1183 at 1385.909 kb on + strand, within Pf6N2E2_1183 at 1385.910 kb on - strand, within Pf6N2E2_1183 at 1385.910 kb on - strand, within Pf6N2E2_1183 at 1385.910 kb on - strand, within Pf6N2E2_1183 at 1385.910 kb on - strand, within Pf6N2E2_1183 at 1385.910 kb on - strand, within Pf6N2E2_1183 at 1385.912 kb on - strand, within Pf6N2E2_1183 at 1386.052 kb on + strand, within Pf6N2E2_1183 at 1386.052 kb on + strand, within Pf6N2E2_1183 at 1386.053 kb on - strand, within Pf6N2E2_1183 at 1386.116 kb on + strand, within Pf6N2E2_1183 at 1386.116 kb on + strand, within Pf6N2E2_1183 at 1386.116 kb on + strand, within Pf6N2E2_1183 at 1386.116 kb on + strand, within Pf6N2E2_1183 at 1386.116 kb on + strand, within Pf6N2E2_1183 at 1386.116 kb on + strand, within Pf6N2E2_1183 at 1386.116 kb on + strand, within Pf6N2E2_1183 at 1386.117 kb on - strand, within Pf6N2E2_1183 at 1386.117 kb on - strand, within Pf6N2E2_1183 at 1386.461 kb on + strand at 1386.615 kb on - strand, within Pf6N2E2_1184 at 1386.689 kb on + strand, within Pf6N2E2_1184 at 1386.764 kb on - strand, within Pf6N2E2_1184 at 1386.832 kb on - strand, within Pf6N2E2_1184 at 1386.938 kb on + strand, within Pf6N2E2_1184 at 1386.938 kb on + strand, within Pf6N2E2_1184 at 1386.939 kb on - strand, within Pf6N2E2_1184 at 1386.939 kb on - strand, within Pf6N2E2_1184 at 1387.033 kb on - strand, within Pf6N2E2_1184 at 1387.059 kb on + strand, within Pf6N2E2_1184 at 1387.154 kb on + strand, within Pf6N2E2_1184 at 1387.155 kb on - strand, within Pf6N2E2_1184 at 1387.232 kb on - strand, within Pf6N2E2_1184 at 1387.313 kb on + strand, within Pf6N2E2_1184 at 1387.313 kb on + strand, within Pf6N2E2_1184 at 1387.360 kb on + strand, within Pf6N2E2_1184 at 1387.361 kb on - strand, within Pf6N2E2_1184
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 1,384,241 + Pf6N2E2_1182 0.10 +0.9 1,384,354 + Pf6N2E2_1182 0.23 -0.6 1,384,354 + Pf6N2E2_1182 0.23 -1.3 1,384,355 - Pf6N2E2_1182 0.23 -3.1 1,384,466 - Pf6N2E2_1182 0.36 -1.7 1,385,116 - +0.2 1,385,126 + +3.1 1,385,127 - +0.3 1,385,127 - -2.4 1,385,387 + Pf6N2E2_1183 0.17 +1.2 1,385,388 - Pf6N2E2_1183 0.17 +1.1 1,385,519 + Pf6N2E2_1183 0.28 -0.3 1,385,519 + Pf6N2E2_1183 0.28 +2.7 1,385,519 + Pf6N2E2_1183 0.28 +2.1 1,385,520 - -0.8 1,385,556 + Pf6N2E2_1183 0.31 +0.9 1,385,769 + Pf6N2E2_1183 0.48 -1.5 1,385,905 + Pf6N2E2_1183 0.59 -1.8 1,385,909 + Pf6N2E2_1183 0.60 +1.2 1,385,909 + Pf6N2E2_1183 0.60 +1.1 1,385,909 + Pf6N2E2_1183 0.60 -2.5 1,385,910 - Pf6N2E2_1183 0.60 -2.1 1,385,910 - Pf6N2E2_1183 0.60 +1.7 1,385,910 - Pf6N2E2_1183 0.60 +0.9 1,385,910 - Pf6N2E2_1183 0.60 +2.4 1,385,910 - Pf6N2E2_1183 0.60 +1.1 1,385,912 - Pf6N2E2_1183 0.60 +2.8 1,386,052 + Pf6N2E2_1183 0.72 -3.3 1,386,052 + Pf6N2E2_1183 0.72 -1.1 1,386,053 - Pf6N2E2_1183 0.72 -1.7 1,386,116 + Pf6N2E2_1183 0.77 -2.1 1,386,116 + Pf6N2E2_1183 0.77 -2.8 1,386,116 + Pf6N2E2_1183 0.77 -0.1 1,386,116 + Pf6N2E2_1183 0.77 +0.5 1,386,116 + Pf6N2E2_1183 0.77 +0.3 1,386,116 + Pf6N2E2_1183 0.77 +2.8 1,386,116 + Pf6N2E2_1183 0.77 -1.5 1,386,117 - Pf6N2E2_1183 0.77 +2.5 1,386,117 - Pf6N2E2_1183 0.77 +3.5 1,386,461 + -0.5 1,386,615 - Pf6N2E2_1184 0.15 -2.1 1,386,689 + Pf6N2E2_1184 0.21 +1.1 1,386,764 - Pf6N2E2_1184 0.27 +0.6 1,386,832 - Pf6N2E2_1184 0.32 +2.4 1,386,938 + Pf6N2E2_1184 0.40 +0.8 1,386,938 + Pf6N2E2_1184 0.40 -2.1 1,386,939 - Pf6N2E2_1184 0.40 -1.7 1,386,939 - Pf6N2E2_1184 0.40 +1.0 1,387,033 - Pf6N2E2_1184 0.47 +0.7 1,387,059 + Pf6N2E2_1184 0.49 -1.7 1,387,154 + Pf6N2E2_1184 0.57 -1.5 1,387,155 - Pf6N2E2_1184 0.57 +1.1 1,387,232 - Pf6N2E2_1184 0.63 -2.5 1,387,313 + Pf6N2E2_1184 0.69 +1.1 1,387,313 + Pf6N2E2_1184 0.69 +1.0 1,387,360 + Pf6N2E2_1184 0.72 +1.3 1,387,361 - Pf6N2E2_1184 0.72 +1.2
Or see this region's nucleotide sequence