Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1108 and Pf6N2E2_1109 are separated by 158 nucleotides Pf6N2E2_1109 and Pf6N2E2_1110 are separated by 340 nucleotides
Pf6N2E2_1108 - Methyltransferase (EC 2.1.1.-), at 1,299,363 to 1,300,421
_1108
Pf6N2E2_1109 - Mobile element protein, at 1,300,580 to 1,301,818
_1109
Pf6N2E2_1110 - FIG00964414: hypothetical protein, at 1,302,159 to 1,302,842
_1110
Position (kb)
1300
1301
1302 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1299.667 kb on + strand, within Pf6N2E2_1108 at 1300.049 kb on - strand, within Pf6N2E2_1108 at 1300.059 kb on + strand, within Pf6N2E2_1108 at 1300.070 kb on + strand, within Pf6N2E2_1108 at 1300.103 kb on + strand, within Pf6N2E2_1108 at 1300.103 kb on + strand, within Pf6N2E2_1108 at 1300.103 kb on + strand, within Pf6N2E2_1108 at 1300.104 kb on - strand, within Pf6N2E2_1108 at 1300.359 kb on + strand at 1300.359 kb on + strand at 1300.360 kb on - strand at 1300.360 kb on - strand at 1300.462 kb on - strand at 1300.490 kb on - strand at 1300.490 kb on - strand at 1300.527 kb on - strand at 1300.536 kb on - strand at 1300.548 kb on + strand at 1300.644 kb on + strand at 1300.645 kb on - strand at 1300.906 kb on - strand, within Pf6N2E2_1109 at 1300.906 kb on - strand, within Pf6N2E2_1109 at 1300.978 kb on - strand, within Pf6N2E2_1109 at 1300.978 kb on - strand, within Pf6N2E2_1109 at 1300.978 kb on - strand, within Pf6N2E2_1109 at 1300.978 kb on - strand, within Pf6N2E2_1109 at 1300.978 kb on - strand, within Pf6N2E2_1109 at 1301.064 kb on + strand, within Pf6N2E2_1109 at 1301.065 kb on - strand, within Pf6N2E2_1109 at 1301.065 kb on - strand, within Pf6N2E2_1109 at 1301.065 kb on - strand, within Pf6N2E2_1109 at 1301.065 kb on - strand, within Pf6N2E2_1109 at 1301.065 kb on - strand, within Pf6N2E2_1109 at 1301.116 kb on - strand, within Pf6N2E2_1109 at 1301.186 kb on - strand, within Pf6N2E2_1109 at 1301.363 kb on + strand, within Pf6N2E2_1109 at 1301.793 kb on + strand at 1301.799 kb on + strand at 1301.799 kb on + strand at 1301.800 kb on - strand at 1301.800 kb on - strand at 1301.805 kb on + strand at 1301.805 kb on + strand at 1301.833 kb on - strand at 1301.943 kb on + strand at 1301.971 kb on + strand at 1301.971 kb on + strand at 1301.972 kb on - strand at 1301.972 kb on - strand at 1302.045 kb on + strand at 1302.046 kb on - strand at 1302.046 kb on - strand at 1302.046 kb on - strand at 1302.046 kb on - strand at 1302.071 kb on - strand at 1302.071 kb on - strand at 1302.160 kb on + strand at 1302.183 kb on - strand at 1302.376 kb on + strand, within Pf6N2E2_1110 at 1302.476 kb on - strand, within Pf6N2E2_1110 at 1302.514 kb on + strand, within Pf6N2E2_1110 at 1302.514 kb on + strand, within Pf6N2E2_1110 at 1302.515 kb on - strand, within Pf6N2E2_1110 at 1302.515 kb on - strand, within Pf6N2E2_1110 at 1302.515 kb on - strand, within Pf6N2E2_1110 at 1302.591 kb on + strand, within Pf6N2E2_1110 at 1302.592 kb on - strand, within Pf6N2E2_1110
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 1,299,667 + Pf6N2E2_1108 0.29 -1.3 1,300,049 - Pf6N2E2_1108 0.65 +0.1 1,300,059 + Pf6N2E2_1108 0.66 -1.1 1,300,070 + Pf6N2E2_1108 0.67 +0.2 1,300,103 + Pf6N2E2_1108 0.70 -0.5 1,300,103 + Pf6N2E2_1108 0.70 -2.4 1,300,103 + Pf6N2E2_1108 0.70 -2.2 1,300,104 - Pf6N2E2_1108 0.70 +0.5 1,300,359 + +0.3 1,300,359 + -1.6 1,300,360 - +0.3 1,300,360 - -1.0 1,300,462 - +2.0 1,300,490 - -1.5 1,300,490 - -0.8 1,300,527 - -2.4 1,300,536 - -2.4 1,300,548 + -0.6 1,300,644 + +1.3 1,300,645 - +0.5 1,300,906 - Pf6N2E2_1109 0.26 +0.2 1,300,906 - Pf6N2E2_1109 0.26 -1.1 1,300,978 - Pf6N2E2_1109 0.32 -1.3 1,300,978 - Pf6N2E2_1109 0.32 -0.5 1,300,978 - Pf6N2E2_1109 0.32 -2.2 1,300,978 - Pf6N2E2_1109 0.32 -1.0 1,300,978 - Pf6N2E2_1109 0.32 -1.9 1,301,064 + Pf6N2E2_1109 0.39 -0.8 1,301,065 - Pf6N2E2_1109 0.39 +1.4 1,301,065 - Pf6N2E2_1109 0.39 -1.4 1,301,065 - Pf6N2E2_1109 0.39 -1.8 1,301,065 - Pf6N2E2_1109 0.39 -0.8 1,301,065 - Pf6N2E2_1109 0.39 +2.3 1,301,116 - Pf6N2E2_1109 0.43 -1.0 1,301,186 - Pf6N2E2_1109 0.49 +0.5 1,301,363 + Pf6N2E2_1109 0.63 -2.3 1,301,793 + -1.0 1,301,799 + -2.2 1,301,799 + -1.9 1,301,800 - -2.4 1,301,800 - -1.5 1,301,805 + +0.4 1,301,805 + -2.5 1,301,833 - -2.8 1,301,943 + +0.2 1,301,971 + -1.6 1,301,971 + -1.3 1,301,972 - -1.0 1,301,972 - +0.3 1,302,045 + +3.1 1,302,046 - +0.5 1,302,046 - -0.0 1,302,046 - -0.5 1,302,046 - +0.2 1,302,071 - -2.2 1,302,071 - -0.5 1,302,160 + -1.0 1,302,183 - -1.0 1,302,376 + Pf6N2E2_1110 0.32 -2.5 1,302,476 - Pf6N2E2_1110 0.46 +0.4 1,302,514 + Pf6N2E2_1110 0.52 -1.8 1,302,514 + Pf6N2E2_1110 0.52 -1.1 1,302,515 - Pf6N2E2_1110 0.52 -2.9 1,302,515 - Pf6N2E2_1110 0.52 +0.8 1,302,515 - Pf6N2E2_1110 0.52 +1.3 1,302,591 + Pf6N2E2_1110 0.63 -2.4 1,302,592 - Pf6N2E2_1110 0.63 -1.0
Or see this region's nucleotide sequence