Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1059 and Pf6N2E2_1060 are separated by 175 nucleotides Pf6N2E2_1060 and Pf6N2E2_1061 overlap by 26 nucleotides Pf6N2E2_1061 and Pf6N2E2_1062 are separated by 130 nucleotides
Pf6N2E2_1059 - Phosphoglucomutase (EC 5.4.2.2), at 1,235,468 to 1,237,114
_1059
Pf6N2E2_1060 - Pirin, at 1,237,290 to 1,237,985
_1060
Pf6N2E2_1061 - hypothetical protein, at 1,237,960 to 1,238,091
_1061
Pf6N2E2_1062 - UvrD/REP helicase family protein, at 1,238,222 to 1,240,708
_1062
Position (kb)
1237
1238 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1236.443 kb on + strand, within Pf6N2E2_1059 at 1236.443 kb on + strand, within Pf6N2E2_1059 at 1236.443 kb on + strand, within Pf6N2E2_1059 at 1236.444 kb on - strand, within Pf6N2E2_1059 at 1236.465 kb on - strand, within Pf6N2E2_1059 at 1236.572 kb on + strand, within Pf6N2E2_1059 at 1236.573 kb on - strand, within Pf6N2E2_1059 at 1236.573 kb on - strand, within Pf6N2E2_1059 at 1236.573 kb on - strand, within Pf6N2E2_1059 at 1236.913 kb on + strand, within Pf6N2E2_1059 at 1237.012 kb on + strand at 1237.012 kb on + strand at 1237.012 kb on + strand at 1237.012 kb on + strand at 1237.013 kb on - strand at 1237.013 kb on - strand at 1237.016 kb on + strand at 1237.016 kb on + strand at 1237.016 kb on + strand at 1237.016 kb on + strand at 1237.016 kb on + strand at 1237.016 kb on + strand at 1237.017 kb on - strand at 1237.017 kb on - strand at 1237.017 kb on - strand at 1237.017 kb on - strand at 1237.017 kb on - strand at 1237.025 kb on + strand at 1237.026 kb on - strand at 1237.099 kb on + strand at 1237.115 kb on + strand at 1237.116 kb on - strand at 1237.116 kb on - strand at 1237.221 kb on - strand at 1237.344 kb on + strand at 1237.456 kb on + strand, within Pf6N2E2_1060 at 1237.638 kb on + strand, within Pf6N2E2_1060 at 1237.638 kb on + strand, within Pf6N2E2_1060 at 1237.639 kb on - strand, within Pf6N2E2_1060 at 1237.639 kb on - strand, within Pf6N2E2_1060 at 1237.675 kb on + strand, within Pf6N2E2_1060 at 1237.675 kb on + strand, within Pf6N2E2_1060 at 1237.676 kb on - strand, within Pf6N2E2_1060 at 1237.676 kb on - strand, within Pf6N2E2_1060 at 1237.676 kb on - strand, within Pf6N2E2_1060 at 1237.676 kb on - strand, within Pf6N2E2_1060 at 1237.676 kb on - strand, within Pf6N2E2_1060 at 1237.716 kb on + strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.900 kb on + strand, within Pf6N2E2_1060 at 1237.991 kb on + strand, within Pf6N2E2_1061 at 1238.031 kb on + strand, within Pf6N2E2_1061 at 1238.131 kb on + strand at 1238.186 kb on + strand at 1238.186 kb on + strand at 1238.187 kb on - strand at 1238.259 kb on + strand at 1238.260 kb on - strand at 1238.390 kb on - strand at 1238.390 kb on - strand at 1238.405 kb on + strand at 1238.405 kb on + strand at 1238.411 kb on + strand at 1238.411 kb on + strand at 1238.411 kb on + strand at 1238.411 kb on + strand at 1238.411 kb on + strand at 1238.412 kb on - strand at 1238.824 kb on + strand, within Pf6N2E2_1062 at 1238.824 kb on + strand, within Pf6N2E2_1062 at 1238.825 kb on - strand, within Pf6N2E2_1062 at 1238.825 kb on - strand, within Pf6N2E2_1062
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 1,236,443 + Pf6N2E2_1059 0.59 +0.3 1,236,443 + Pf6N2E2_1059 0.59 -3.0 1,236,443 + Pf6N2E2_1059 0.59 -2.0 1,236,444 - Pf6N2E2_1059 0.59 -1.6 1,236,465 - Pf6N2E2_1059 0.61 -3.3 1,236,572 + Pf6N2E2_1059 0.67 -2.0 1,236,573 - Pf6N2E2_1059 0.67 +1.1 1,236,573 - Pf6N2E2_1059 0.67 +0.7 1,236,573 - Pf6N2E2_1059 0.67 +0.3 1,236,913 + Pf6N2E2_1059 0.88 -3.0 1,237,012 + -1.3 1,237,012 + +1.1 1,237,012 + +3.0 1,237,012 + +1.4 1,237,013 - -1.7 1,237,013 - +1.4 1,237,016 + +0.5 1,237,016 + +3.5 1,237,016 + +1.4 1,237,016 + -1.9 1,237,016 + -1.5 1,237,016 + -1.9 1,237,017 - -1.9 1,237,017 - -0.5 1,237,017 - +0.4 1,237,017 - +0.5 1,237,017 - -3.3 1,237,025 + -0.0 1,237,026 - +1.6 1,237,099 + -2.5 1,237,115 + +1.3 1,237,116 - -0.8 1,237,116 - +0.5 1,237,221 - -3.3 1,237,344 + +1.5 1,237,456 + Pf6N2E2_1060 0.24 +1.8 1,237,638 + Pf6N2E2_1060 0.50 +1.7 1,237,638 + Pf6N2E2_1060 0.50 -0.6 1,237,639 - Pf6N2E2_1060 0.50 +0.2 1,237,639 - Pf6N2E2_1060 0.50 -1.5 1,237,675 + Pf6N2E2_1060 0.55 +1.7 1,237,675 + Pf6N2E2_1060 0.55 -1.4 1,237,676 - Pf6N2E2_1060 0.55 +1.1 1,237,676 - Pf6N2E2_1060 0.55 -0.7 1,237,676 - Pf6N2E2_1060 0.55 -1.5 1,237,676 - Pf6N2E2_1060 0.55 +2.0 1,237,676 - Pf6N2E2_1060 0.55 +1.2 1,237,716 + Pf6N2E2_1060 0.61 +0.8 1,237,717 - Pf6N2E2_1060 0.61 -0.2 1,237,717 - Pf6N2E2_1060 0.61 -0.1 1,237,717 - Pf6N2E2_1060 0.61 -2.4 1,237,717 - Pf6N2E2_1060 0.61 +1.2 1,237,717 - Pf6N2E2_1060 0.61 -0.9 1,237,717 - Pf6N2E2_1060 0.61 -2.2 1,237,717 - Pf6N2E2_1060 0.61 -0.8 1,237,717 - Pf6N2E2_1060 0.61 +1.5 1,237,786 + Pf6N2E2_1060 0.71 -0.0 1,237,786 + Pf6N2E2_1060 0.71 +0.5 1,237,786 + Pf6N2E2_1060 0.71 -1.3 1,237,786 + Pf6N2E2_1060 0.71 -0.9 1,237,786 + Pf6N2E2_1060 0.71 +1.2 1,237,786 + Pf6N2E2_1060 0.71 -2.5 1,237,786 + Pf6N2E2_1060 0.71 -0.2 1,237,786 + Pf6N2E2_1060 0.71 +0.6 1,237,787 - Pf6N2E2_1060 0.71 +1.8 1,237,787 - Pf6N2E2_1060 0.71 -3.7 1,237,787 - Pf6N2E2_1060 0.71 +1.2 1,237,787 - Pf6N2E2_1060 0.71 -0.5 1,237,787 - Pf6N2E2_1060 0.71 +1.0 1,237,787 - Pf6N2E2_1060 0.71 -1.0 1,237,787 - Pf6N2E2_1060 0.71 +2.4 1,237,787 - Pf6N2E2_1060 0.71 +0.5 1,237,787 - Pf6N2E2_1060 0.71 +0.7 1,237,787 - Pf6N2E2_1060 0.71 -1.3 1,237,787 - Pf6N2E2_1060 0.71 -1.6 1,237,900 + Pf6N2E2_1060 0.88 +0.8 1,237,991 + Pf6N2E2_1061 0.23 -1.5 1,238,031 + Pf6N2E2_1061 0.54 -1.5 1,238,131 + -2.2 1,238,186 + -0.4 1,238,186 + -1.8 1,238,187 - +0.3 1,238,259 + -0.0 1,238,260 - +2.8 1,238,390 - -1.3 1,238,390 - +0.6 1,238,405 + +1.1 1,238,405 + -1.6 1,238,411 + -0.3 1,238,411 + +1.8 1,238,411 + -1.5 1,238,411 + -0.8 1,238,411 + -2.9 1,238,412 - -0.0 1,238,824 + Pf6N2E2_1062 0.24 -0.0 1,238,824 + Pf6N2E2_1062 0.24 -1.9 1,238,825 - Pf6N2E2_1062 0.24 -2.4 1,238,825 - Pf6N2E2_1062 0.24 -2.3
Or see this region's nucleotide sequence