Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_104 and Pf6N2E2_105 overlap by 4 nucleotides Pf6N2E2_105 and Pf6N2E2_106 are separated by 10 nucleotides Pf6N2E2_106 and Pf6N2E2_107 are separated by 10 nucleotides Pf6N2E2_107 and Pf6N2E2_108 are separated by 48 nucleotides
Pf6N2E2_104 - Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3), at 149,638 to 150,747
_104
Pf6N2E2_105 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 150,744 to 151,610
_105
Pf6N2E2_106 - Glycerol-3-phosphate ABC transporter, permease protein UgpE (TC 3.A.1.1.3), at 151,621 to 152,421
_106
Pf6N2E2_107 - Probable transmembrane protein, at 152,432 to 152,704
_107
Pf6N2E2_108 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 152,753 to 154,495
_108
Position (kb)
151
152
153 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 150.627 kb on - strand, within Pf6N2E2_104 at 150.667 kb on + strand at 150.667 kb on + strand at 150.667 kb on + strand at 150.701 kb on - strand at 150.712 kb on + strand at 150.712 kb on + strand at 150.879 kb on + strand, within Pf6N2E2_105 at 151.167 kb on + strand, within Pf6N2E2_105 at 151.167 kb on + strand, within Pf6N2E2_105 at 151.168 kb on - strand, within Pf6N2E2_105 at 151.168 kb on - strand, within Pf6N2E2_105 at 151.173 kb on + strand, within Pf6N2E2_105 at 151.173 kb on + strand, within Pf6N2E2_105 at 151.174 kb on - strand, within Pf6N2E2_105 at 151.174 kb on - strand, within Pf6N2E2_105 at 151.174 kb on - strand, within Pf6N2E2_105 at 151.258 kb on - strand, within Pf6N2E2_105 at 151.287 kb on + strand, within Pf6N2E2_105 at 151.288 kb on - strand, within Pf6N2E2_105 at 151.288 kb on - strand, within Pf6N2E2_105 at 151.290 kb on + strand, within Pf6N2E2_105 at 151.290 kb on + strand at 151.290 kb on + strand, within Pf6N2E2_105 at 151.291 kb on - strand, within Pf6N2E2_105 at 151.413 kb on + strand, within Pf6N2E2_105 at 151.414 kb on - strand, within Pf6N2E2_105 at 151.540 kb on - strand at 151.540 kb on - strand at 151.540 kb on - strand at 151.540 kb on - strand at 151.575 kb on + strand at 151.575 kb on + strand at 151.654 kb on + strand at 151.655 kb on - strand at 151.655 kb on - strand at 151.655 kb on - strand at 151.681 kb on + strand at 151.695 kb on + strand at 151.695 kb on + strand at 151.696 kb on - strand at 151.768 kb on + strand, within Pf6N2E2_106 at 151.768 kb on + strand, within Pf6N2E2_106 at 151.768 kb on + strand, within Pf6N2E2_106 at 151.769 kb on - strand, within Pf6N2E2_106 at 151.798 kb on + strand, within Pf6N2E2_106 at 151.823 kb on - strand, within Pf6N2E2_106 at 151.825 kb on + strand, within Pf6N2E2_106 at 151.826 kb on - strand, within Pf6N2E2_106 at 151.826 kb on - strand, within Pf6N2E2_106 at 151.984 kb on + strand, within Pf6N2E2_106 at 151.984 kb on + strand, within Pf6N2E2_106 at 151.985 kb on - strand, within Pf6N2E2_106 at 151.985 kb on - strand, within Pf6N2E2_106 at 152.117 kb on - strand, within Pf6N2E2_106 at 152.703 kb on - strand at 152.731 kb on + strand at 152.752 kb on + strand at 152.753 kb on - strand at 152.753 kb on - strand at 152.753 kb on - strand at 152.753 kb on - strand at 152.782 kb on + strand at 152.783 kb on - strand at 152.783 kb on - strand at 152.893 kb on + strand at 152.893 kb on + strand at 153.127 kb on + strand, within Pf6N2E2_108 at 153.127 kb on + strand, within Pf6N2E2_108 at 153.127 kb on + strand, within Pf6N2E2_108 at 153.127 kb on + strand, within Pf6N2E2_108 at 153.127 kb on + strand, within Pf6N2E2_108 at 153.127 kb on + strand, within Pf6N2E2_108 at 153.127 kb on + strand, within Pf6N2E2_108 at 153.128 kb on - strand, within Pf6N2E2_108 at 153.128 kb on - strand, within Pf6N2E2_108 at 153.128 kb on - strand, within Pf6N2E2_108 at 153.128 kb on - strand, within Pf6N2E2_108 at 153.128 kb on - strand, within Pf6N2E2_108 at 153.128 kb on - strand, within Pf6N2E2_108 at 153.128 kb on - strand, within Pf6N2E2_108 at 153.131 kb on + strand, within Pf6N2E2_108 at 153.131 kb on + strand, within Pf6N2E2_108 at 153.131 kb on + strand, within Pf6N2E2_108 at 153.131 kb on + strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.132 kb on - strand, within Pf6N2E2_108 at 153.268 kb on + strand, within Pf6N2E2_108 at 153.278 kb on - strand, within Pf6N2E2_108 at 153.336 kb on - strand, within Pf6N2E2_108 at 153.395 kb on + strand, within Pf6N2E2_108 at 153.395 kb on + strand, within Pf6N2E2_108 at 153.396 kb on - strand, within Pf6N2E2_108 at 153.396 kb on - strand, within Pf6N2E2_108 at 153.396 kb on - strand, within Pf6N2E2_108
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 150,627 - Pf6N2E2_104 0.89 +0.4 150,667 + -2.1 150,667 + -0.7 150,667 + -3.1 150,701 - +1.2 150,712 + +1.5 150,712 + -1.3 150,879 + Pf6N2E2_105 0.16 -0.5 151,167 + Pf6N2E2_105 0.49 -0.4 151,167 + Pf6N2E2_105 0.49 -0.8 151,168 - Pf6N2E2_105 0.49 +1.1 151,168 - Pf6N2E2_105 0.49 +0.6 151,173 + Pf6N2E2_105 0.49 -1.7 151,173 + Pf6N2E2_105 0.49 -2.6 151,174 - Pf6N2E2_105 0.50 +0.5 151,174 - Pf6N2E2_105 0.50 +0.5 151,174 - Pf6N2E2_105 0.50 -1.6 151,258 - Pf6N2E2_105 0.59 -2.1 151,287 + Pf6N2E2_105 0.63 -1.1 151,288 - Pf6N2E2_105 0.63 +0.6 151,288 - Pf6N2E2_105 0.63 +0.5 151,290 + Pf6N2E2_105 0.63 +0.3 151,290 + +0.2 151,290 + Pf6N2E2_105 0.63 +0.9 151,291 - Pf6N2E2_105 0.63 +1.1 151,413 + Pf6N2E2_105 0.77 -0.3 151,414 - Pf6N2E2_105 0.77 +0.7 151,540 - +2.1 151,540 - +0.5 151,540 - -1.3 151,540 - -1.3 151,575 + +0.0 151,575 + -1.3 151,654 + -2.8 151,655 - +1.2 151,655 - +1.1 151,655 - -2.2 151,681 + -3.0 151,695 + -2.0 151,695 + +0.8 151,696 - +0.8 151,768 + Pf6N2E2_106 0.18 -3.2 151,768 + Pf6N2E2_106 0.18 -0.2 151,768 + Pf6N2E2_106 0.18 +1.2 151,769 - Pf6N2E2_106 0.18 +0.1 151,798 + Pf6N2E2_106 0.22 +0.7 151,823 - Pf6N2E2_106 0.25 -0.2 151,825 + Pf6N2E2_106 0.25 -1.1 151,826 - Pf6N2E2_106 0.26 -2.2 151,826 - Pf6N2E2_106 0.26 +1.1 151,984 + Pf6N2E2_106 0.45 -2.4 151,984 + Pf6N2E2_106 0.45 -0.4 151,985 - Pf6N2E2_106 0.45 +1.7 151,985 - Pf6N2E2_106 0.45 -0.5 152,117 - Pf6N2E2_106 0.62 -1.2 152,703 - +0.7 152,731 + +2.1 152,752 + +1.3 152,753 - -1.3 152,753 - +0.2 152,753 - +0.2 152,753 - +1.4 152,782 + +1.0 152,783 - -1.3 152,783 - +1.2 152,893 + +0.5 152,893 + -2.2 153,127 + Pf6N2E2_108 0.21 -1.6 153,127 + Pf6N2E2_108 0.21 -2.2 153,127 + Pf6N2E2_108 0.21 -0.5 153,127 + Pf6N2E2_108 0.21 +0.4 153,127 + Pf6N2E2_108 0.21 +1.2 153,127 + Pf6N2E2_108 0.21 +2.0 153,127 + Pf6N2E2_108 0.21 +2.0 153,128 - Pf6N2E2_108 0.22 -1.1 153,128 - Pf6N2E2_108 0.22 +0.4 153,128 - Pf6N2E2_108 0.22 +3.2 153,128 - Pf6N2E2_108 0.22 -2.9 153,128 - Pf6N2E2_108 0.22 -2.6 153,128 - Pf6N2E2_108 0.22 -1.8 153,128 - Pf6N2E2_108 0.22 -0.4 153,131 + Pf6N2E2_108 0.22 +1.0 153,131 + Pf6N2E2_108 0.22 +0.5 153,131 + Pf6N2E2_108 0.22 -1.2 153,131 + Pf6N2E2_108 0.22 +2.3 153,132 - Pf6N2E2_108 0.22 -2.2 153,132 - Pf6N2E2_108 0.22 +2.5 153,132 - Pf6N2E2_108 0.22 +1.5 153,132 - Pf6N2E2_108 0.22 -2.0 153,132 - Pf6N2E2_108 0.22 -1.4 153,132 - Pf6N2E2_108 0.22 -0.3 153,132 - Pf6N2E2_108 0.22 +2.7 153,132 - Pf6N2E2_108 0.22 +0.2 153,132 - Pf6N2E2_108 0.22 -0.7 153,132 - Pf6N2E2_108 0.22 -1.3 153,268 + Pf6N2E2_108 0.30 -2.4 153,278 - Pf6N2E2_108 0.30 +2.0 153,336 - Pf6N2E2_108 0.33 -3.9 153,395 + Pf6N2E2_108 0.37 +0.8 153,395 + Pf6N2E2_108 0.37 -0.9 153,396 - Pf6N2E2_108 0.37 -0.9 153,396 - Pf6N2E2_108 0.37 +0.9 153,396 - Pf6N2E2_108 0.37 -0.8
Or see this region's nucleotide sequence