Experiment: Bile salts 0.0125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1040 and Pf6N2E2_1041 overlap by 4 nucleotides Pf6N2E2_1041 and Pf6N2E2_1042 are separated by 87 nucleotides
Pf6N2E2_1040 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 1,214,067 to 1,215,008
_1040
Pf6N2E2_1041 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 1,215,005 to 1,216,204
_1041
Pf6N2E2_1042 - Nitrate/nitrite transporter, at 1,216,292 to 1,217,611
_1042
Position (kb)
1215
1216
1217 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1214.075 kb on + strand at 1214.076 kb on - strand at 1214.076 kb on - strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.086 kb on + strand at 1214.087 kb on - strand at 1214.087 kb on - strand at 1214.087 kb on - strand at 1214.090 kb on + strand at 1214.090 kb on + strand at 1214.091 kb on - strand at 1214.091 kb on - strand at 1214.091 kb on - strand at 1214.366 kb on + strand, within Pf6N2E2_1040 at 1214.513 kb on - strand, within Pf6N2E2_1040 at 1214.787 kb on - strand, within Pf6N2E2_1040 at 1215.069 kb on + strand at 1215.160 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.203 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.377 kb on + strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.378 kb on - strand, within Pf6N2E2_1041 at 1215.410 kb on + strand, within Pf6N2E2_1041 at 1215.464 kb on + strand, within Pf6N2E2_1041 at 1215.464 kb on + strand, within Pf6N2E2_1041 at 1215.464 kb on + strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.465 kb on - strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.713 kb on + strand, within Pf6N2E2_1041 at 1215.728 kb on - strand at 1216.155 kb on - strand at 1216.264 kb on - strand at 1216.266 kb on - strand at 1216.272 kb on - strand at 1216.272 kb on - strand at 1216.415 kb on + strand at 1216.416 kb on - strand at 1216.416 kb on - strand at 1216.427 kb on + strand, within Pf6N2E2_1042 at 1216.428 kb on - strand, within Pf6N2E2_1042 at 1216.439 kb on - strand, within Pf6N2E2_1042 at 1216.519 kb on + strand, within Pf6N2E2_1042 at 1216.519 kb on + strand, within Pf6N2E2_1042 at 1216.519 kb on + strand, within Pf6N2E2_1042 at 1216.520 kb on - strand, within Pf6N2E2_1042 at 1216.520 kb on - strand, within Pf6N2E2_1042 at 1216.520 kb on - strand, within Pf6N2E2_1042 at 1216.520 kb on - strand, within Pf6N2E2_1042 at 1216.520 kb on - strand, within Pf6N2E2_1042 at 1216.520 kb on - strand, within Pf6N2E2_1042 at 1216.568 kb on + strand, within Pf6N2E2_1042 at 1216.655 kb on + strand, within Pf6N2E2_1042 at 1216.655 kb on + strand, within Pf6N2E2_1042 at 1216.664 kb on + strand, within Pf6N2E2_1042 at 1216.665 kb on - strand, within Pf6N2E2_1042 at 1217.108 kb on + strand, within Pf6N2E2_1042 at 1217.192 kb on + strand, within Pf6N2E2_1042
Per-strain Table
Position Strand Gene LocusTag Fraction Bile salts 0.0125 M remove 1,214,075 + -2.8 1,214,076 - -2.3 1,214,076 - -1.0 1,214,086 + -2.1 1,214,086 + +1.6 1,214,086 + +0.6 1,214,086 + +1.9 1,214,086 + -0.3 1,214,086 + -2.3 1,214,086 + -2.0 1,214,087 - +1.3 1,214,087 - -1.6 1,214,087 - -0.3 1,214,090 + +0.8 1,214,090 + -1.3 1,214,091 - -2.6 1,214,091 - -0.1 1,214,091 - -0.2 1,214,366 + Pf6N2E2_1040 0.32 +1.2 1,214,513 - Pf6N2E2_1040 0.47 +3.0 1,214,787 - Pf6N2E2_1040 0.76 +0.2 1,215,069 + +0.3 1,215,160 + Pf6N2E2_1041 0.13 -1.8 1,215,203 + Pf6N2E2_1041 0.17 -1.9 1,215,203 + Pf6N2E2_1041 0.17 -0.3 1,215,203 + Pf6N2E2_1041 0.17 +0.9 1,215,203 + Pf6N2E2_1041 0.17 -2.0 1,215,203 + Pf6N2E2_1041 0.17 +3.2 1,215,203 + Pf6N2E2_1041 0.17 +0.1 1,215,377 + Pf6N2E2_1041 0.31 -1.4 1,215,377 + Pf6N2E2_1041 0.31 +2.2 1,215,377 + Pf6N2E2_1041 0.31 -3.4 1,215,377 + Pf6N2E2_1041 0.31 -1.8 1,215,377 + Pf6N2E2_1041 0.31 +1.8 1,215,377 + Pf6N2E2_1041 0.31 -0.5 1,215,378 - Pf6N2E2_1041 0.31 -1.4 1,215,378 - Pf6N2E2_1041 0.31 +0.7 1,215,378 - Pf6N2E2_1041 0.31 -0.1 1,215,378 - Pf6N2E2_1041 0.31 +2.1 1,215,378 - Pf6N2E2_1041 0.31 -0.3 1,215,410 + Pf6N2E2_1041 0.34 +0.6 1,215,464 + Pf6N2E2_1041 0.38 +1.4 1,215,464 + Pf6N2E2_1041 0.38 +0.8 1,215,464 + Pf6N2E2_1041 0.38 -1.4 1,215,465 - Pf6N2E2_1041 0.38 +1.6 1,215,465 - Pf6N2E2_1041 0.38 +2.4 1,215,465 - Pf6N2E2_1041 0.38 +0.2 1,215,465 - Pf6N2E2_1041 0.38 +1.1 1,215,713 + Pf6N2E2_1041 0.59 +2.0 1,215,713 + Pf6N2E2_1041 0.59 +1.9 1,215,713 + Pf6N2E2_1041 0.59 +0.7 1,215,713 + Pf6N2E2_1041 0.59 -1.8 1,215,728 - +0.6 1,216,155 - +2.6 1,216,264 - +0.6 1,216,266 - -0.4 1,216,272 - +2.8 1,216,272 - -2.7 1,216,415 + +2.4 1,216,416 - +0.6 1,216,416 - -0.6 1,216,427 + Pf6N2E2_1042 0.10 -2.6 1,216,428 - Pf6N2E2_1042 0.10 -0.8 1,216,439 - Pf6N2E2_1042 0.11 +2.0 1,216,519 + Pf6N2E2_1042 0.17 -3.1 1,216,519 + Pf6N2E2_1042 0.17 -0.8 1,216,519 + Pf6N2E2_1042 0.17 -0.4 1,216,520 - Pf6N2E2_1042 0.17 -0.0 1,216,520 - Pf6N2E2_1042 0.17 -1.6 1,216,520 - Pf6N2E2_1042 0.17 +0.6 1,216,520 - Pf6N2E2_1042 0.17 -1.0 1,216,520 - Pf6N2E2_1042 0.17 +2.0 1,216,520 - Pf6N2E2_1042 0.17 -3.2 1,216,568 + Pf6N2E2_1042 0.21 +1.4 1,216,655 + Pf6N2E2_1042 0.28 -2.0 1,216,655 + Pf6N2E2_1042 0.28 -2.5 1,216,664 + Pf6N2E2_1042 0.28 -1.7 1,216,665 - Pf6N2E2_1042 0.28 +1.2 1,217,108 + Pf6N2E2_1042 0.62 -0.2 1,217,192 + Pf6N2E2_1042 0.68 -0.9
Or see this region's nucleotide sequence