Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS24535

Experiment: Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS24530 and BWI76_RS24535 are separated by 46 nucleotidesBWI76_RS24535 and BWI76_RS24540 are separated by 8 nucleotidesBWI76_RS24540 and BWI76_RS24545 are separated by 13 nucleotidesBWI76_RS24545 and BWI76_RS24550 are separated by 233 nucleotides BWI76_RS24530: BWI76_RS24530 - cytosine/purines uracil thiamine allantoin permease, at 5,113,275 to 5,114,882 _RS24530 BWI76_RS24535: BWI76_RS24535 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 5,114,929 to 5,115,942 _RS24535 BWI76_RS24540: BWI76_RS24540 - hypothetical protein, at 5,115,951 to 5,116,166 _RS24540 BWI76_RS24545: BWI76_RS24545 - 30S ribosomal protein S21, at 5,116,180 to 5,116,395 _RS24545 BWI76_RS24550: BWI76_RS24550 - DNA primase, at 5,116,629 to 5,118,374 _RS24550 Position (kb) 5114 5115 5116Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 5113.941 kb on - strand, within BWI76_RS24530at 5113.945 kb on + strand, within BWI76_RS24530at 5113.953 kb on - strand, within BWI76_RS24530at 5114.064 kb on + strand, within BWI76_RS24530at 5114.064 kb on + strand, within BWI76_RS24530at 5114.148 kb on + strand, within BWI76_RS24530at 5114.150 kb on + strand, within BWI76_RS24530at 5114.150 kb on + strand, within BWI76_RS24530at 5114.156 kb on - strandat 5114.174 kb on - strand, within BWI76_RS24530at 5114.229 kb on - strand, within BWI76_RS24530at 5114.372 kb on - strand, within BWI76_RS24530at 5114.381 kb on + strand, within BWI76_RS24530at 5114.389 kb on - strand, within BWI76_RS24530at 5114.428 kb on + strand, within BWI76_RS24530at 5114.565 kb on + strand, within BWI76_RS24530at 5114.710 kb on + strand, within BWI76_RS24530at 5114.718 kb on - strand, within BWI76_RS24530at 5114.757 kb on - strandat 5114.762 kb on - strandat 5114.864 kb on + strandat 5114.864 kb on + strandat 5115.956 kb on - strandat 5115.982 kb on - strand, within BWI76_RS24540at 5115.982 kb on - strand, within BWI76_RS24540at 5115.982 kb on - strand, within BWI76_RS24540at 5115.982 kb on - strand, within BWI76_RS24540at 5115.982 kb on - strand, within BWI76_RS24540at 5115.982 kb on - strand, within BWI76_RS24540at 5115.982 kb on - strand, within BWI76_RS24540at 5116.003 kb on - strand, within BWI76_RS24540at 5116.012 kb on + strand, within BWI76_RS24540at 5116.017 kb on + strand, within BWI76_RS24540at 5116.526 kb on + strandat 5116.531 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM
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5,113,941 - BWI76_RS24530 0.41 +0.8
5,113,945 + BWI76_RS24530 0.42 -2.2
5,113,953 - BWI76_RS24530 0.42 -0.6
5,114,064 + BWI76_RS24530 0.49 -1.3
5,114,064 + BWI76_RS24530 0.49 -0.5
5,114,148 + BWI76_RS24530 0.54 -0.7
5,114,150 + BWI76_RS24530 0.54 +0.2
5,114,150 + BWI76_RS24530 0.54 -0.3
5,114,156 - +2.9
5,114,174 - BWI76_RS24530 0.56 +0.2
5,114,229 - BWI76_RS24530 0.59 +0.2
5,114,372 - BWI76_RS24530 0.68 +0.3
5,114,381 + BWI76_RS24530 0.69 -2.9
5,114,389 - BWI76_RS24530 0.69 -0.1
5,114,428 + BWI76_RS24530 0.72 +0.2
5,114,565 + BWI76_RS24530 0.80 -2.9
5,114,710 + BWI76_RS24530 0.89 -0.4
5,114,718 - BWI76_RS24530 0.90 +2.5
5,114,757 - +0.1
5,114,762 - +0.2
5,114,864 + -0.5
5,114,864 + +0.5
5,115,956 - +0.8
5,115,982 - BWI76_RS24540 0.14 +1.8
5,115,982 - BWI76_RS24540 0.14 +1.2
5,115,982 - BWI76_RS24540 0.14 +0.3
5,115,982 - BWI76_RS24540 0.14 +1.0
5,115,982 - BWI76_RS24540 0.14 +0.7
5,115,982 - BWI76_RS24540 0.14 -3.3
5,115,982 - BWI76_RS24540 0.14 +1.1
5,116,003 - BWI76_RS24540 0.24 -0.1
5,116,012 + BWI76_RS24540 0.28 +0.2
5,116,017 + BWI76_RS24540 0.31 +1.1
5,116,526 + -3.1
5,116,531 + -0.9

Or see this region's nucleotide sequence