Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21305 and BWI76_RS21310 are separated by 11 nucleotides BWI76_RS21310 and BWI76_RS21315 are separated by 11 nucleotides BWI76_RS21315 and BWI76_RS21320 overlap by 4 nucleotides BWI76_RS21320 and BWI76_RS21325 overlap by 57 nucleotides BWI76_RS21325 and BWI76_RS21330 are separated by 50 nucleotides
BWI76_RS21305: BWI76_RS21305 - pyridoxine 5'-phosphate synthase, at 4,461,706 to 4,462,437
_RS21305
BWI76_RS21310: BWI76_RS21310 - DNA repair protein RecO, at 4,462,449 to 4,463,186
_RS21310
BWI76_RS21315: BWI76_RS21315 - GTPase Era, at 4,463,198 to 4,464,103
_RS21315
BWI76_RS21320: BWI76_RS21320 - ribonuclease 3, at 4,464,100 to 4,464,780
_RS21320
BWI76_RS21325: BWI76_RS21325 - hypothetical protein, at 4,464,724 to 4,464,954
_RS21325
BWI76_RS21330: BWI76_RS21330 - S26 family signal peptidase, at 4,465,005 to 4,465,979
_RS21330
Position (kb) 4463
4464
4465 Strain fitness (log2 ratio) -2
-1
0
1 at 4462.497 kb on - strand at 4463.024 kb on - strand, within BWI76_RS21310 at 4464.783 kb on - strand, within BWI76_RS21325 at 4464.922 kb on + strand, within BWI76_RS21325 at 4464.992 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 4,462,497 - +1.0 4,463,024 - BWI76_RS21310 0.78 +0.5 4,464,783 - BWI76_RS21325 0.26 -0.9 4,464,922 + BWI76_RS21325 0.86 -1.9 4,464,992 - +1.5
Or see this region's nucleotide sequence