Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21275 and BWI76_RS21280 overlap by 4 nucleotides BWI76_RS21280 and BWI76_RS21285 are separated by 19 nucleotides BWI76_RS21285 and BWI76_RS21290 are separated by 209 nucleotides
BWI76_RS21275: BWI76_RS21275 - lytic transglycosylase F, at 4,456,991 to 4,458,607
_RS21275
BWI76_RS21280: BWI76_RS21280 - tRNA adenosine(34) deaminase TadA, at 4,458,604 to 4,459,149
_RS21280
BWI76_RS21285: BWI76_RS21285 - HAD family hydrolase, at 4,459,169 to 4,459,804
_RS21285
BWI76_RS21290: BWI76_RS21290 - DNA-binding transcriptional regulator, at 4,460,014 to 4,460,862
_RS21290
Position (kb) 4458
4459
4460 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4457.617 kb on - strand, within BWI76_RS21275 at 4457.684 kb on + strand, within BWI76_RS21275 at 4457.738 kb on - strand, within BWI76_RS21275 at 4458.374 kb on + strand, within BWI76_RS21275 at 4458.380 kb on + strand, within BWI76_RS21275 at 4458.382 kb on - strand, within BWI76_RS21275 at 4458.492 kb on - strand at 4458.607 kb on + strand at 4459.171 kb on + strand at 4459.398 kb on + strand, within BWI76_RS21285 at 4459.745 kb on - strand at 4459.988 kb on + strand at 4459.996 kb on - strand at 4460.011 kb on + strand at 4460.045 kb on + strand at 4460.053 kb on - strand at 4460.053 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 4,457,617 - BWI76_RS21275 0.39 -3.5 4,457,684 + BWI76_RS21275 0.43 -1.5 4,457,738 - BWI76_RS21275 0.46 -2.6 4,458,374 + BWI76_RS21275 0.86 -1.7 4,458,380 + BWI76_RS21275 0.86 -1.6 4,458,382 - BWI76_RS21275 0.86 -0.7 4,458,492 - -1.8 4,458,607 + +2.2 4,459,171 + -0.9 4,459,398 + BWI76_RS21285 0.36 -1.2 4,459,745 - -1.4 4,459,988 + -0.2 4,459,996 - -0.6 4,460,011 + -0.3 4,460,045 + -0.4 4,460,053 - +0.5 4,460,053 - -0.6
Or see this region's nucleotide sequence