Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14530

Experiment: Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14525 and BWI76_RS14530 are separated by 182 nucleotidesBWI76_RS14530 and BWI76_RS14535 are separated by 86 nucleotides BWI76_RS14525: BWI76_RS14525 - AraC family transcriptional regulator, at 3,034,876 to 3,035,835 _RS14525 BWI76_RS14530: BWI76_RS14530 - non-ribosomal peptide synthetase, at 3,036,018 to 3,042,116 _RS14530 BWI76_RS14535: BWI76_RS14535 - polyketide synthase, at 3,042,203 to 3,051,679 _RS14535 Position (kb) 3036 3037 3038 3039 3040 3041 3042 3043Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3035.061 kb on + strand, within BWI76_RS14525at 3035.205 kb on - strand, within BWI76_RS14525at 3035.230 kb on - strand, within BWI76_RS14525at 3035.456 kb on + strand, within BWI76_RS14525at 3035.754 kb on - strandat 3035.754 kb on - strandat 3036.162 kb on + strandat 3036.170 kb on - strandat 3036.170 kb on - strandat 3036.808 kb on - strand, within BWI76_RS14530at 3037.203 kb on - strand, within BWI76_RS14530at 3037.410 kb on + strand, within BWI76_RS14530at 3037.418 kb on - strand, within BWI76_RS14530at 3037.468 kb on + strand, within BWI76_RS14530at 3037.902 kb on - strand, within BWI76_RS14530at 3037.921 kb on - strand, within BWI76_RS14530at 3037.931 kb on + strand, within BWI76_RS14530at 3038.152 kb on + strand, within BWI76_RS14530at 3038.205 kb on - strand, within BWI76_RS14530at 3038.247 kb on + strandat 3038.259 kb on + strand, within BWI76_RS14530at 3038.336 kb on + strand, within BWI76_RS14530at 3038.344 kb on - strand, within BWI76_RS14530at 3038.575 kb on - strand, within BWI76_RS14530at 3038.609 kb on - strand, within BWI76_RS14530at 3038.706 kb on + strand, within BWI76_RS14530at 3038.903 kb on + strand, within BWI76_RS14530at 3039.485 kb on - strand, within BWI76_RS14530at 3039.616 kb on + strand, within BWI76_RS14530at 3039.716 kb on + strand, within BWI76_RS14530at 3039.851 kb on + strand, within BWI76_RS14530at 3039.893 kb on + strand, within BWI76_RS14530at 3039.946 kb on + strand, within BWI76_RS14530at 3040.416 kb on - strand, within BWI76_RS14530at 3040.447 kb on - strand, within BWI76_RS14530at 3040.721 kb on + strand, within BWI76_RS14530at 3041.161 kb on + strand, within BWI76_RS14530at 3041.484 kb on + strand, within BWI76_RS14530at 3041.578 kb on - strandat 3041.591 kb on + strandat 3041.697 kb on - strandat 3041.716 kb on + strandat 3041.999 kb on + strandat 3042.461 kb on + strandat 3042.688 kb on + strandat 3042.688 kb on + strandat 3042.696 kb on - strandat 3042.785 kb on - strandat 3042.836 kb on - strandat 3042.962 kb on - strandat 3043.110 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM
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3,035,061 + BWI76_RS14525 0.19 +0.6
3,035,205 - BWI76_RS14525 0.34 +1.0
3,035,230 - BWI76_RS14525 0.37 +1.7
3,035,456 + BWI76_RS14525 0.60 -1.2
3,035,754 - +0.2
3,035,754 - -0.2
3,036,162 + -1.0
3,036,170 - +0.5
3,036,170 - +2.4
3,036,808 - BWI76_RS14530 0.13 +0.1
3,037,203 - BWI76_RS14530 0.19 +0.7
3,037,410 + BWI76_RS14530 0.23 -1.8
3,037,418 - BWI76_RS14530 0.23 -0.9
3,037,468 + BWI76_RS14530 0.24 +0.3
3,037,902 - BWI76_RS14530 0.31 -0.4
3,037,921 - BWI76_RS14530 0.31 +0.7
3,037,931 + BWI76_RS14530 0.31 -0.4
3,038,152 + BWI76_RS14530 0.35 +0.7
3,038,205 - BWI76_RS14530 0.36 +0.2
3,038,247 + -0.7
3,038,259 + BWI76_RS14530 0.37 -1.1
3,038,336 + BWI76_RS14530 0.38 +0.7
3,038,344 - BWI76_RS14530 0.38 +1.4
3,038,575 - BWI76_RS14530 0.42 -0.3
3,038,609 - BWI76_RS14530 0.42 -0.4
3,038,706 + BWI76_RS14530 0.44 -0.1
3,038,903 + BWI76_RS14530 0.47 -0.1
3,039,485 - BWI76_RS14530 0.57 +0.7
3,039,616 + BWI76_RS14530 0.59 -0.1
3,039,716 + BWI76_RS14530 0.61 +1.0
3,039,851 + BWI76_RS14530 0.63 +2.8
3,039,893 + BWI76_RS14530 0.64 +1.1
3,039,946 + BWI76_RS14530 0.64 +0.1
3,040,416 - BWI76_RS14530 0.72 -0.1
3,040,447 - BWI76_RS14530 0.73 -0.4
3,040,721 + BWI76_RS14530 0.77 -3.3
3,041,161 + BWI76_RS14530 0.84 -0.1
3,041,484 + BWI76_RS14530 0.90 -1.2
3,041,578 - +2.0
3,041,591 + +0.0
3,041,697 - -0.3
3,041,716 + +1.6
3,041,999 + -3.1
3,042,461 + -0.3
3,042,688 + +0.9
3,042,688 + +0.6
3,042,696 - +0.5
3,042,785 - -0.1
3,042,836 - +0.1
3,042,962 - +1.4
3,043,110 - +0.6

Or see this region's nucleotide sequence