Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13155 and BWI76_RS13160 are separated by 35 nucleotides BWI76_RS13160 and BWI76_RS13165 are separated by 204 nucleotides BWI76_RS13165 and BWI76_RS13170 are separated by 48 nucleotides BWI76_RS13170 and BWI76_RS13175 are separated by 15 nucleotides
BWI76_RS13155: BWI76_RS13155 - hypothetical protein, at 2,743,127 to 2,743,534
_RS13155
BWI76_RS13160: BWI76_RS13160 - azoreductase, at 2,743,570 to 2,744,175
_RS13160
BWI76_RS13165: BWI76_RS13165 - ATP-dependent RNA helicase HrpA, at 2,744,380 to 2,748,282
_RS13165
BWI76_RS13170: BWI76_RS13170 - methyltransferase, at 2,748,331 to 2,748,990
_RS13170
BWI76_RS13175: BWI76_RS13175 - NodT family RND efflux system outer membrane lipoprotein, at 2,749,006 to 2,750,385
_RS13175
Position (kb)
2744
2745
2746
2747
2748
2749 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2743.707 kb on + strand, within BWI76_RS13160 at 2744.165 kb on + strand at 2744.220 kb on - strand at 2744.892 kb on + strand, within BWI76_RS13165 at 2745.143 kb on - strand, within BWI76_RS13165 at 2745.368 kb on - strand, within BWI76_RS13165 at 2745.704 kb on + strand, within BWI76_RS13165 at 2745.771 kb on - strand, within BWI76_RS13165 at 2745.947 kb on - strand, within BWI76_RS13165 at 2746.641 kb on + strand, within BWI76_RS13165 at 2746.641 kb on + strand, within BWI76_RS13165 at 2746.649 kb on - strand, within BWI76_RS13165 at 2747.271 kb on + strand, within BWI76_RS13165 at 2747.329 kb on + strand, within BWI76_RS13165 at 2747.419 kb on - strand, within BWI76_RS13165 at 2748.216 kb on + strand at 2748.513 kb on + strand, within BWI76_RS13170 at 2748.726 kb on + strand, within BWI76_RS13170 at 2748.768 kb on - strand, within BWI76_RS13170
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 2,743,707 + BWI76_RS13160 0.23 +0.2 2,744,165 + +0.6 2,744,220 - +1.6 2,744,892 + BWI76_RS13165 0.13 -3.4 2,745,143 - BWI76_RS13165 0.20 -1.8 2,745,368 - BWI76_RS13165 0.25 -1.8 2,745,704 + BWI76_RS13165 0.34 -1.0 2,745,771 - BWI76_RS13165 0.36 -1.3 2,745,947 - BWI76_RS13165 0.40 -3.9 2,746,641 + BWI76_RS13165 0.58 -0.7 2,746,641 + BWI76_RS13165 0.58 -0.2 2,746,649 - BWI76_RS13165 0.58 +0.6 2,747,271 + BWI76_RS13165 0.74 -1.5 2,747,329 + BWI76_RS13165 0.76 -4.9 2,747,419 - BWI76_RS13165 0.78 -1.6 2,748,216 + -0.0 2,748,513 + BWI76_RS13170 0.28 -0.1 2,748,726 + BWI76_RS13170 0.60 +2.3 2,748,768 - BWI76_RS13170 0.66 +0.8
Or see this region's nucleotide sequence