Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11365

Experiment: Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11360 and BWI76_RS11365 are separated by 36 nucleotidesBWI76_RS11365 and BWI76_RS11370 are separated by 0 nucleotidesBWI76_RS11370 and BWI76_RS11375 overlap by 4 nucleotides BWI76_RS11360: BWI76_RS11360 - L-asparaginase, at 2,394,301 to 2,395,344 _RS11360 BWI76_RS11365: BWI76_RS11365 - iron ABC transporter, at 2,395,381 to 2,396,148 _RS11365 BWI76_RS11370: BWI76_RS11370 - iron-dicitrate transporter subunit FecD, at 2,396,149 to 2,397,102 _RS11370 BWI76_RS11375: BWI76_RS11375 - iron-dicitrate transporter permease subunit, at 2,397,099 to 2,398,097 _RS11375 Position (kb) 2395 2396 2397Strain fitness (log2 ratio) -2 -1 0 1at 2394.477 kb on + strand, within BWI76_RS11360at 2394.477 kb on + strand, within BWI76_RS11360at 2394.485 kb on - strand, within BWI76_RS11360at 2394.498 kb on + strand, within BWI76_RS11360at 2394.598 kb on - strand, within BWI76_RS11360at 2395.329 kb on + strandat 2395.541 kb on + strand, within BWI76_RS11365at 2395.551 kb on + strand, within BWI76_RS11365at 2395.784 kb on - strand, within BWI76_RS11365at 2396.128 kb on - strandat 2396.177 kb on + strandat 2396.210 kb on + strandat 2396.870 kb on + strand, within BWI76_RS11370at 2397.009 kb on + strandat 2397.136 kb on + strandat 2397.144 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM
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2,394,477 + BWI76_RS11360 0.17 +0.3
2,394,477 + BWI76_RS11360 0.17 -0.9
2,394,485 - BWI76_RS11360 0.18 +0.1
2,394,498 + BWI76_RS11360 0.19 -0.0
2,394,598 - BWI76_RS11360 0.28 -0.4
2,395,329 + +0.5
2,395,541 + BWI76_RS11365 0.21 -2.4
2,395,551 + BWI76_RS11365 0.22 -0.5
2,395,784 - BWI76_RS11365 0.52 -0.4
2,396,128 - -1.3
2,396,177 + -1.9
2,396,210 + +0.0
2,396,870 + BWI76_RS11370 0.76 -1.2
2,397,009 + -2.0
2,397,136 + +0.9
2,397,144 - +0.1

Or see this region's nucleotide sequence