Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09745

Experiment: Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09740 and BWI76_RS09745 are separated by 382 nucleotidesBWI76_RS09745 and BWI76_RS09750 are separated by 114 nucleotidesBWI76_RS09750 and BWI76_RS09755 are separated by 186 nucleotides BWI76_RS09740: BWI76_RS09740 - formate transporter FocA, at 2,042,161 to 2,043,018 _RS09740 BWI76_RS09745: BWI76_RS09745 - 30S ribosomal protein S12 methylthiotransferase accessory protein YcaO, at 2,043,401 to 2,045,161 _RS09745 BWI76_RS09750: BWI76_RS09750 - DUF421 domain-containing protein, at 2,045,276 to 2,045,968 _RS09750 BWI76_RS09755: BWI76_RS09755 - phosphoserine transaminase, at 2,046,155 to 2,047,243 _RS09755 Position (kb) 2043 2044 2045 2046Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2042.484 kb on + strand, within BWI76_RS09740at 2042.584 kb on + strand, within BWI76_RS09740at 2042.672 kb on - strand, within BWI76_RS09740at 2042.754 kb on + strand, within BWI76_RS09740at 2042.864 kb on - strand, within BWI76_RS09740at 2042.884 kb on + strand, within BWI76_RS09740at 2042.884 kb on + strand, within BWI76_RS09740at 2042.884 kb on + strand, within BWI76_RS09740at 2042.884 kb on + strand, within BWI76_RS09740at 2042.892 kb on - strand, within BWI76_RS09740at 2042.892 kb on - strand, within BWI76_RS09740at 2042.892 kb on - strand, within BWI76_RS09740at 2042.892 kb on - strand, within BWI76_RS09740at 2042.892 kb on - strand, within BWI76_RS09740at 2042.900 kb on - strand, within BWI76_RS09740at 2042.902 kb on - strand, within BWI76_RS09740at 2042.902 kb on - strand, within BWI76_RS09740at 2042.910 kb on + strand, within BWI76_RS09740at 2042.976 kb on + strandat 2042.978 kb on + strandat 2043.004 kb on + strandat 2043.018 kb on + strandat 2043.026 kb on - strandat 2043.028 kb on + strandat 2043.030 kb on - strandat 2043.030 kb on - strandat 2043.063 kb on + strandat 2043.063 kb on + strandat 2043.071 kb on - strandat 2043.071 kb on - strandat 2043.071 kb on - strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.088 kb on + strandat 2043.096 kb on - strandat 2043.096 kb on - strandat 2043.096 kb on - strandat 2043.096 kb on - strandat 2043.096 kb on - strandat 2043.097 kb on - strandat 2043.097 kb on - strandat 2043.121 kb on - strandat 2043.345 kb on + strandat 2043.447 kb on + strandat 2043.455 kb on - strandat 2043.530 kb on - strandat 2043.613 kb on - strand, within BWI76_RS09745at 2043.635 kb on + strand, within BWI76_RS09745at 2043.689 kb on + strand, within BWI76_RS09745at 2043.711 kb on - strand, within BWI76_RS09745at 2043.716 kb on - strand, within BWI76_RS09745at 2043.778 kb on + strand, within BWI76_RS09745at 2043.786 kb on - strand, within BWI76_RS09745at 2044.025 kb on + strand, within BWI76_RS09745at 2044.097 kb on + strand, within BWI76_RS09745at 2044.234 kb on + strand, within BWI76_RS09745at 2044.386 kb on - strand, within BWI76_RS09745at 2044.549 kb on - strand, within BWI76_RS09745at 2045.018 kb on + strandat 2045.018 kb on + strandat 2045.026 kb on - strandat 2045.041 kb on + strandat 2045.041 kb on + strandat 2045.259 kb on - strandat 2045.259 kb on - strandat 2045.337 kb on + strandat 2045.347 kb on + strand, within BWI76_RS09750at 2045.354 kb on + strand, within BWI76_RS09750at 2045.361 kb on - strand, within BWI76_RS09750at 2045.433 kb on - strand, within BWI76_RS09750at 2045.490 kb on + strand, within BWI76_RS09750at 2045.778 kb on - strand, within BWI76_RS09750at 2045.796 kb on + strand, within BWI76_RS09750at 2045.934 kb on + strandat 2045.999 kb on - strandat 2046.054 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM
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2,042,484 + BWI76_RS09740 0.38 +0.2
2,042,584 + BWI76_RS09740 0.49 -0.0
2,042,672 - BWI76_RS09740 0.60 -1.3
2,042,754 + BWI76_RS09740 0.69 -2.3
2,042,864 - BWI76_RS09740 0.82 -2.1
2,042,884 + BWI76_RS09740 0.84 -2.9
2,042,884 + BWI76_RS09740 0.84 +0.6
2,042,884 + BWI76_RS09740 0.84 +1.1
2,042,884 + BWI76_RS09740 0.84 +0.1
2,042,892 - BWI76_RS09740 0.85 +0.5
2,042,892 - BWI76_RS09740 0.85 +1.3
2,042,892 - BWI76_RS09740 0.85 -0.5
2,042,892 - BWI76_RS09740 0.85 -0.0
2,042,892 - BWI76_RS09740 0.85 -3.1
2,042,900 - BWI76_RS09740 0.86 -1.1
2,042,902 - BWI76_RS09740 0.86 -0.0
2,042,902 - BWI76_RS09740 0.86 +0.2
2,042,910 + BWI76_RS09740 0.87 -0.7
2,042,976 + +0.0
2,042,978 + +2.2
2,043,004 + -2.4
2,043,018 + -1.5
2,043,026 - -1.2
2,043,028 + +1.6
2,043,030 - -0.3
2,043,030 - -0.7
2,043,063 + -0.1
2,043,063 + +1.1
2,043,071 - -1.1
2,043,071 - +2.6
2,043,071 - +0.3
2,043,088 + +0.3
2,043,088 + -0.5
2,043,088 + +2.1
2,043,088 + +1.9
2,043,088 + +1.2
2,043,088 + +1.0
2,043,088 + +2.9
2,043,088 + +1.6
2,043,088 + +0.3
2,043,088 + -0.0
2,043,096 - -2.2
2,043,096 - -1.3
2,043,096 - +0.3
2,043,096 - +0.7
2,043,096 - +1.8
2,043,097 - +0.8
2,043,097 - -0.0
2,043,121 - -1.0
2,043,345 + +1.2
2,043,447 + +1.5
2,043,455 - +1.4
2,043,530 - +0.6
2,043,613 - BWI76_RS09745 0.12 +0.9
2,043,635 + BWI76_RS09745 0.13 +1.1
2,043,689 + BWI76_RS09745 0.16 -1.8
2,043,711 - BWI76_RS09745 0.18 +0.8
2,043,716 - BWI76_RS09745 0.18 +0.8
2,043,778 + BWI76_RS09745 0.21 +1.3
2,043,786 - BWI76_RS09745 0.22 +0.9
2,044,025 + BWI76_RS09745 0.35 +1.0
2,044,097 + BWI76_RS09745 0.40 +1.3
2,044,234 + BWI76_RS09745 0.47 +1.0
2,044,386 - BWI76_RS09745 0.56 +1.7
2,044,549 - BWI76_RS09745 0.65 +1.5
2,045,018 + +1.3
2,045,018 + +1.6
2,045,026 - +0.7
2,045,041 + +1.4
2,045,041 + -0.9
2,045,259 - +0.2
2,045,259 - -0.8
2,045,337 + -1.7
2,045,347 + BWI76_RS09750 0.10 -0.5
2,045,354 + BWI76_RS09750 0.11 -0.0
2,045,361 - BWI76_RS09750 0.12 -1.0
2,045,433 - BWI76_RS09750 0.23 -0.1
2,045,490 + BWI76_RS09750 0.31 -0.0
2,045,778 - BWI76_RS09750 0.72 -0.6
2,045,796 + BWI76_RS09750 0.75 -1.5
2,045,934 + +0.6
2,045,999 - -0.5
2,046,054 - -2.4

Or see this region's nucleotide sequence