Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05435 and BWI76_RS05440 are separated by 71 nucleotides BWI76_RS05440 and BWI76_RS05445 are separated by 32 nucleotides BWI76_RS05445 and BWI76_RS05450 overlap by 4 nucleotides BWI76_RS05450 and BWI76_RS05455 are separated by 65 nucleotides
BWI76_RS05435: BWI76_RS05435 - murein transglycosylase D, at 1,141,016 to 1,142,383
_RS05435
BWI76_RS05440: BWI76_RS05440 - hydroxyacylglutathione hydrolase, at 1,142,455 to 1,143,210
_RS05440
BWI76_RS05445: BWI76_RS05445 - type 11 methyltransferase, at 1,143,243 to 1,143,965
_RS05445
BWI76_RS05450: BWI76_RS05450 - ribonuclease HI, at 1,143,962 to 1,144,429
_RS05450
BWI76_RS05455: BWI76_RS05455 - DNA polymerase III subunit epsilon, at 1,144,495 to 1,145,226
_RS05455
Position (kb)
1143
1144 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1142.278 kb on - strand at 1142.305 kb on + strand at 1142.384 kb on - strand at 1142.384 kb on - strand at 1142.453 kb on - strand at 1142.469 kb on + strand at 1142.491 kb on + strand at 1142.491 kb on + strand at 1142.499 kb on - strand at 1142.499 kb on - strand at 1142.637 kb on - strand, within BWI76_RS05440 at 1142.692 kb on + strand, within BWI76_RS05440 at 1142.715 kb on + strand, within BWI76_RS05440 at 1142.718 kb on - strand, within BWI76_RS05440 at 1142.736 kb on - strand, within BWI76_RS05440 at 1142.750 kb on + strand, within BWI76_RS05440 at 1142.800 kb on - strand, within BWI76_RS05440 at 1142.800 kb on - strand, within BWI76_RS05440 at 1142.800 kb on - strand, within BWI76_RS05440 at 1142.841 kb on + strand, within BWI76_RS05440 at 1142.879 kb on - strand, within BWI76_RS05440 at 1142.879 kb on - strand, within BWI76_RS05440 at 1143.029 kb on + strand, within BWI76_RS05440 at 1143.076 kb on - strand, within BWI76_RS05440 at 1143.264 kb on - strand at 1143.294 kb on + strand at 1143.352 kb on - strand, within BWI76_RS05445 at 1143.352 kb on - strand, within BWI76_RS05445 at 1143.470 kb on + strand, within BWI76_RS05445 at 1143.470 kb on + strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.622 kb on + strand, within BWI76_RS05445 at 1143.641 kb on + strand, within BWI76_RS05445 at 1144.210 kb on + strand, within BWI76_RS05450 at 1144.317 kb on - strand, within BWI76_RS05450 at 1144.492 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 1,142,278 - +1.0 1,142,305 + -2.5 1,142,384 - -1.2 1,142,384 - -0.5 1,142,453 - -0.4 1,142,469 + -1.4 1,142,491 + -0.0 1,142,491 + -1.8 1,142,499 - +1.2 1,142,499 - +0.5 1,142,637 - BWI76_RS05440 0.24 +0.1 1,142,692 + BWI76_RS05440 0.31 +0.5 1,142,715 + BWI76_RS05440 0.34 -4.3 1,142,718 - BWI76_RS05440 0.35 -0.0 1,142,736 - BWI76_RS05440 0.37 +0.3 1,142,750 + BWI76_RS05440 0.39 -1.3 1,142,800 - BWI76_RS05440 0.46 -0.3 1,142,800 - BWI76_RS05440 0.46 +1.5 1,142,800 - BWI76_RS05440 0.46 +0.4 1,142,841 + BWI76_RS05440 0.51 +2.1 1,142,879 - BWI76_RS05440 0.56 +0.6 1,142,879 - BWI76_RS05440 0.56 +0.4 1,143,029 + BWI76_RS05440 0.76 -0.7 1,143,076 - BWI76_RS05440 0.82 +1.4 1,143,264 - +0.9 1,143,294 + +0.4 1,143,352 - BWI76_RS05445 0.15 -0.6 1,143,352 - BWI76_RS05445 0.15 -0.6 1,143,470 + BWI76_RS05445 0.31 +0.1 1,143,470 + BWI76_RS05445 0.31 +1.1 1,143,478 - BWI76_RS05445 0.33 +0.8 1,143,478 - BWI76_RS05445 0.33 +0.5 1,143,478 - BWI76_RS05445 0.33 +0.9 1,143,478 - BWI76_RS05445 0.33 +0.9 1,143,622 + BWI76_RS05445 0.52 -1.8 1,143,641 + BWI76_RS05445 0.55 -1.4 1,144,210 + BWI76_RS05450 0.53 +3.9 1,144,317 - BWI76_RS05450 0.76 +4.9 1,144,492 + -0.2
Or see this region's nucleotide sequence