Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05440

Experiment: Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05435 and BWI76_RS05440 are separated by 71 nucleotidesBWI76_RS05440 and BWI76_RS05445 are separated by 32 nucleotidesBWI76_RS05445 and BWI76_RS05450 overlap by 4 nucleotides BWI76_RS05435: BWI76_RS05435 - murein transglycosylase D, at 1,141,016 to 1,142,383 _RS05435 BWI76_RS05440: BWI76_RS05440 - hydroxyacylglutathione hydrolase, at 1,142,455 to 1,143,210 _RS05440 BWI76_RS05445: BWI76_RS05445 - type 11 methyltransferase, at 1,143,243 to 1,143,965 _RS05445 BWI76_RS05450: BWI76_RS05450 - ribonuclease HI, at 1,143,962 to 1,144,429 _RS05450 Position (kb) 1142 1143 1144Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 1141.503 kb on - strand, within BWI76_RS05435at 1141.592 kb on + strand, within BWI76_RS05435at 1141.632 kb on - strand, within BWI76_RS05435at 1141.795 kb on - strand, within BWI76_RS05435at 1141.987 kb on + strand, within BWI76_RS05435at 1141.995 kb on - strand, within BWI76_RS05435at 1142.204 kb on - strand, within BWI76_RS05435at 1142.208 kb on - strand, within BWI76_RS05435at 1142.230 kb on + strand, within BWI76_RS05435at 1142.230 kb on + strand, within BWI76_RS05435at 1142.278 kb on - strandat 1142.305 kb on + strandat 1142.384 kb on - strandat 1142.384 kb on - strandat 1142.453 kb on - strandat 1142.469 kb on + strandat 1142.491 kb on + strandat 1142.491 kb on + strandat 1142.499 kb on - strandat 1142.499 kb on - strandat 1142.637 kb on - strand, within BWI76_RS05440at 1142.692 kb on + strand, within BWI76_RS05440at 1142.715 kb on + strand, within BWI76_RS05440at 1142.718 kb on - strand, within BWI76_RS05440at 1142.736 kb on - strand, within BWI76_RS05440at 1142.750 kb on + strand, within BWI76_RS05440at 1142.800 kb on - strand, within BWI76_RS05440at 1142.800 kb on - strand, within BWI76_RS05440at 1142.800 kb on - strand, within BWI76_RS05440at 1142.841 kb on + strand, within BWI76_RS05440at 1142.879 kb on - strand, within BWI76_RS05440at 1142.879 kb on - strand, within BWI76_RS05440at 1143.029 kb on + strand, within BWI76_RS05440at 1143.076 kb on - strand, within BWI76_RS05440at 1143.264 kb on - strandat 1143.294 kb on + strandat 1143.352 kb on - strand, within BWI76_RS05445at 1143.352 kb on - strand, within BWI76_RS05445at 1143.470 kb on + strand, within BWI76_RS05445at 1143.470 kb on + strand, within BWI76_RS05445at 1143.478 kb on - strand, within BWI76_RS05445at 1143.478 kb on - strand, within BWI76_RS05445at 1143.478 kb on - strand, within BWI76_RS05445at 1143.478 kb on - strand, within BWI76_RS05445at 1143.622 kb on + strand, within BWI76_RS05445at 1143.641 kb on + strand, within BWI76_RS05445at 1144.210 kb on + strand, within BWI76_RS05450

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM
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1,141,503 - BWI76_RS05435 0.36 -0.2
1,141,592 + BWI76_RS05435 0.42 +0.5
1,141,632 - BWI76_RS05435 0.45 -0.5
1,141,795 - BWI76_RS05435 0.57 -2.2
1,141,987 + BWI76_RS05435 0.71 -0.8
1,141,995 - BWI76_RS05435 0.72 -1.6
1,142,204 - BWI76_RS05435 0.87 +0.6
1,142,208 - BWI76_RS05435 0.87 -0.3
1,142,230 + BWI76_RS05435 0.89 +0.5
1,142,230 + BWI76_RS05435 0.89 -1.0
1,142,278 - +1.0
1,142,305 + -2.5
1,142,384 - -1.2
1,142,384 - -0.5
1,142,453 - -0.4
1,142,469 + -1.4
1,142,491 + -0.0
1,142,491 + -1.8
1,142,499 - +1.2
1,142,499 - +0.5
1,142,637 - BWI76_RS05440 0.24 +0.1
1,142,692 + BWI76_RS05440 0.31 +0.5
1,142,715 + BWI76_RS05440 0.34 -4.3
1,142,718 - BWI76_RS05440 0.35 -0.0
1,142,736 - BWI76_RS05440 0.37 +0.3
1,142,750 + BWI76_RS05440 0.39 -1.3
1,142,800 - BWI76_RS05440 0.46 -0.3
1,142,800 - BWI76_RS05440 0.46 +1.5
1,142,800 - BWI76_RS05440 0.46 +0.4
1,142,841 + BWI76_RS05440 0.51 +2.1
1,142,879 - BWI76_RS05440 0.56 +0.6
1,142,879 - BWI76_RS05440 0.56 +0.4
1,143,029 + BWI76_RS05440 0.76 -0.7
1,143,076 - BWI76_RS05440 0.82 +1.4
1,143,264 - +0.9
1,143,294 + +0.4
1,143,352 - BWI76_RS05445 0.15 -0.6
1,143,352 - BWI76_RS05445 0.15 -0.6
1,143,470 + BWI76_RS05445 0.31 +0.1
1,143,470 + BWI76_RS05445 0.31 +1.1
1,143,478 - BWI76_RS05445 0.33 +0.8
1,143,478 - BWI76_RS05445 0.33 +0.5
1,143,478 - BWI76_RS05445 0.33 +0.9
1,143,478 - BWI76_RS05445 0.33 +0.9
1,143,622 + BWI76_RS05445 0.52 -1.8
1,143,641 + BWI76_RS05445 0.55 -1.4
1,144,210 + BWI76_RS05450 0.53 +3.9

Or see this region's nucleotide sequence