Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02005

Experiment: Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01995 and BWI76_RS02000 are separated by 84 nucleotidesBWI76_RS02000 and BWI76_RS02005 are separated by 258 nucleotidesBWI76_RS02005 and BWI76_RS02010 are separated by 36 nucleotidesBWI76_RS02010 and BWI76_RS02015 are separated by 151 nucleotides BWI76_RS01995: BWI76_RS01995 - AraC family transcriptional regulator, at 410,724 to 411,053 _RS01995 BWI76_RS02000: BWI76_RS02000 - transcriptional regulator, at 411,138 to 411,596 _RS02000 BWI76_RS02005: BWI76_RS02005 - glutathione S-transferase, at 411,855 to 412,523 _RS02005 BWI76_RS02010: BWI76_RS02010 - hypothetical protein, at 412,560 to 412,760 _RS02010 BWI76_RS02015: BWI76_RS02015 - NCS2 family permease, at 412,912 to 414,261 _RS02015 Position (kb) 411 412 413Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 410.915 kb on - strand, within BWI76_RS01995at 411.091 kb on - strandat 411.314 kb on + strand, within BWI76_RS02000at 411.385 kb on + strand, within BWI76_RS02000at 411.393 kb on - strand, within BWI76_RS02000at 411.393 kb on - strand, within BWI76_RS02000at 411.454 kb on + strand, within BWI76_RS02000at 411.454 kb on + strand, within BWI76_RS02000at 411.454 kb on + strand, within BWI76_RS02000at 411.454 kb on + strand, within BWI76_RS02000at 411.454 kb on + strand, within BWI76_RS02000at 411.462 kb on - strand, within BWI76_RS02000at 411.462 kb on - strand, within BWI76_RS02000at 411.462 kb on - strand, within BWI76_RS02000at 411.462 kb on - strand, within BWI76_RS02000at 411.699 kb on + strandat 411.699 kb on + strandat 411.699 kb on + strandat 411.699 kb on + strandat 411.702 kb on - strandat 411.707 kb on - strandat 411.707 kb on - strandat 411.707 kb on - strandat 411.707 kb on - strandat 411.727 kb on + strandat 411.864 kb on - strandat 411.943 kb on - strand, within BWI76_RS02005at 411.951 kb on - strand, within BWI76_RS02005at 411.953 kb on + strand, within BWI76_RS02005at 411.959 kb on + strand, within BWI76_RS02005at 412.056 kb on + strand, within BWI76_RS02005at 412.078 kb on + strand, within BWI76_RS02005at 412.085 kb on - strand, within BWI76_RS02005at 412.145 kb on + strand, within BWI76_RS02005at 412.150 kb on + strand, within BWI76_RS02005at 412.213 kb on - strand, within BWI76_RS02005at 412.253 kb on + strand, within BWI76_RS02005at 412.261 kb on - strand, within BWI76_RS02005at 412.285 kb on - strand, within BWI76_RS02005at 412.322 kb on + strand, within BWI76_RS02005at 412.461 kb on - strandat 412.463 kb on + strandat 412.863 kb on - strandat 413.015 kb on + strandat 413.016 kb on - strandat 413.130 kb on + strand, within BWI76_RS02015at 413.138 kb on - strand, within BWI76_RS02015at 413.390 kb on + strand, within BWI76_RS02015

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM
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410,915 - BWI76_RS01995 0.58 -2.4
411,091 - +2.6
411,314 + BWI76_RS02000 0.38 +0.9
411,385 + BWI76_RS02000 0.54 -1.4
411,393 - BWI76_RS02000 0.56 +0.1
411,393 - BWI76_RS02000 0.56 +0.3
411,454 + BWI76_RS02000 0.69 -2.7
411,454 + BWI76_RS02000 0.69 -2.7
411,454 + BWI76_RS02000 0.69 -0.4
411,454 + BWI76_RS02000 0.69 -0.5
411,454 + BWI76_RS02000 0.69 -2.0
411,462 - BWI76_RS02000 0.71 -1.0
411,462 - BWI76_RS02000 0.71 -1.0
411,462 - BWI76_RS02000 0.71 -0.9
411,462 - BWI76_RS02000 0.71 +1.1
411,699 + +0.0
411,699 + -3.8
411,699 + -2.0
411,699 + +1.1
411,702 - +0.3
411,707 - +1.0
411,707 - -0.8
411,707 - -0.5
411,707 - +0.2
411,727 + -0.2
411,864 - -1.1
411,943 - BWI76_RS02005 0.13 +0.5
411,951 - BWI76_RS02005 0.14 +0.1
411,953 + BWI76_RS02005 0.15 -2.1
411,959 + BWI76_RS02005 0.16 -0.9
412,056 + BWI76_RS02005 0.30 -1.9
412,078 + BWI76_RS02005 0.33 -1.7
412,085 - BWI76_RS02005 0.34 +0.3
412,145 + BWI76_RS02005 0.43 +0.9
412,150 + BWI76_RS02005 0.44 +1.3
412,213 - BWI76_RS02005 0.54 -2.9
412,253 + BWI76_RS02005 0.59 -2.5
412,261 - BWI76_RS02005 0.61 +2.2
412,285 - BWI76_RS02005 0.64 +0.7
412,322 + BWI76_RS02005 0.70 +1.4
412,461 - -0.2
412,463 + +1.2
412,863 - -1.1
413,015 + -0.3
413,016 - -0.9
413,130 + BWI76_RS02015 0.16 -0.2
413,138 - BWI76_RS02015 0.17 -0.5
413,390 + BWI76_RS02015 0.35 -0.4

Or see this region's nucleotide sequence