Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00875 and BWI76_RS00880 are separated by 15 nucleotides BWI76_RS00880 and BWI76_RS00885 are separated by 46 nucleotides BWI76_RS00885 and BWI76_RS00890 are separated by 364 nucleotides
BWI76_RS00875: BWI76_RS00875 - DNA-binding transcriptional regulator FabR, at 180,927 to 181,562
_RS00875
BWI76_RS00880: BWI76_RS00880 - membrane protein, at 181,578 to 181,937
_RS00880
BWI76_RS00885: BWI76_RS00885 - tRNA (uridine(54)-C5)-methyltransferase TrmA, at 181,984 to 183,084
_RS00885
BWI76_RS00890: BWI76_RS00890 - vitamin B12/cobalamin outer membrane transporter, at 183,449 to 185,317
_RS00890
Position (kb) 181
182
183
184 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 181.021 kb on + strand, within BWI76_RS00875 at 181.021 kb on + strand, within BWI76_RS00875 at 181.021 kb on + strand, within BWI76_RS00875 at 181.109 kb on + strand, within BWI76_RS00875 at 181.129 kb on + strand, within BWI76_RS00875 at 181.294 kb on + strand, within BWI76_RS00875 at 181.383 kb on + strand, within BWI76_RS00875 at 181.388 kb on + strand, within BWI76_RS00875 at 181.396 kb on - strand, within BWI76_RS00875 at 181.444 kb on + strand, within BWI76_RS00875 at 181.586 kb on - strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.595 kb on + strand at 181.595 kb on + strand at 181.598 kb on - strand at 181.603 kb on - strand at 181.659 kb on + strand, within BWI76_RS00880 at 181.661 kb on + strand, within BWI76_RS00880 at 181.661 kb on + strand, within BWI76_RS00880 at 181.886 kb on + strand, within BWI76_RS00880 at 182.436 kb on + strand, within BWI76_RS00885 at 182.439 kb on - strand, within BWI76_RS00885 at 182.575 kb on - strand, within BWI76_RS00885 at 182.637 kb on + strand, within BWI76_RS00885 at 182.713 kb on + strand, within BWI76_RS00885 at 182.772 kb on + strand, within BWI76_RS00885 at 182.812 kb on - strand, within BWI76_RS00885 at 183.052 kb on + strand at 183.062 kb on + strand at 183.078 kb on - strand at 183.079 kb on + strand at 183.121 kb on - strand at 183.129 kb on + strand at 183.140 kb on + strand at 183.140 kb on + strand at 183.140 kb on + strand at 183.145 kb on + strand at 183.231 kb on + strand at 183.236 kb on + strand at 183.290 kb on - strand at 183.328 kb on + strand at 183.354 kb on - strand at 183.360 kb on - strand at 183.413 kb on - strand at 183.419 kb on - strand at 183.428 kb on + strand at 183.527 kb on - strand at 183.535 kb on + strand at 183.536 kb on - strand at 183.567 kb on + strand at 183.613 kb on - strand at 183.753 kb on - strand, within BWI76_RS00890 at 183.758 kb on + strand, within BWI76_RS00890 at 183.786 kb on + strand, within BWI76_RS00890 at 183.830 kb on + strand, within BWI76_RS00890 at 183.872 kb on + strand, within BWI76_RS00890 at 183.880 kb on - strand, within BWI76_RS00890 at 183.894 kb on - strand, within BWI76_RS00890 at 183.942 kb on + strand, within BWI76_RS00890 at 183.942 kb on + strand, within BWI76_RS00890 at 183.942 kb on + strand, within BWI76_RS00890 at 183.945 kb on - strand, within BWI76_RS00890 at 183.951 kb on - strand, within BWI76_RS00890 at 184.005 kb on - strand, within BWI76_RS00890 at 184.017 kb on + strand, within BWI76_RS00890 at 184.017 kb on + strand, within BWI76_RS00890 at 184.066 kb on + strand, within BWI76_RS00890
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 181,021 + BWI76_RS00875 0.15 -3.2 181,021 + BWI76_RS00875 0.15 -4.1 181,021 + BWI76_RS00875 0.15 -0.6 181,109 + BWI76_RS00875 0.29 -1.3 181,129 + BWI76_RS00875 0.32 -3.5 181,294 + BWI76_RS00875 0.58 -4.1 181,383 + BWI76_RS00875 0.72 -3.7 181,388 + BWI76_RS00875 0.72 -3.7 181,396 - BWI76_RS00875 0.74 -0.7 181,444 + BWI76_RS00875 0.81 -2.7 181,586 - -1.7 181,590 + +0.9 181,590 + -1.6 181,590 + +1.5 181,590 + +0.7 181,590 + +0.4 181,595 + +0.5 181,595 + +1.1 181,598 - -1.0 181,603 - +0.6 181,659 + BWI76_RS00880 0.23 +1.8 181,661 + BWI76_RS00880 0.23 +1.5 181,661 + BWI76_RS00880 0.23 -1.6 181,886 + BWI76_RS00880 0.86 +0.9 182,436 + BWI76_RS00885 0.41 -1.9 182,439 - BWI76_RS00885 0.41 -0.8 182,575 - BWI76_RS00885 0.54 +0.2 182,637 + BWI76_RS00885 0.59 -0.6 182,713 + BWI76_RS00885 0.66 -0.7 182,772 + BWI76_RS00885 0.72 -1.1 182,812 - BWI76_RS00885 0.75 -1.7 183,052 + -0.9 183,062 + +0.7 183,078 - -1.7 183,079 + -0.2 183,121 - -0.6 183,129 + +2.6 183,140 + +0.3 183,140 + -1.6 183,140 + -0.6 183,145 + +1.0 183,231 + +0.6 183,236 + -0.9 183,290 - -0.6 183,328 + +1.6 183,354 - +1.0 183,360 - +1.6 183,413 - -3.0 183,419 - -2.8 183,428 + -1.1 183,527 - +1.2 183,535 + -0.4 183,536 - +0.3 183,567 + -1.5 183,613 - +0.5 183,753 - BWI76_RS00890 0.16 -0.6 183,758 + BWI76_RS00890 0.17 +0.5 183,786 + BWI76_RS00890 0.18 -0.4 183,830 + BWI76_RS00890 0.20 -0.2 183,872 + BWI76_RS00890 0.23 -2.1 183,880 - BWI76_RS00890 0.23 +0.6 183,894 - BWI76_RS00890 0.24 -0.1 183,942 + BWI76_RS00890 0.26 -0.5 183,942 + BWI76_RS00890 0.26 -0.8 183,942 + BWI76_RS00890 0.26 +0.0 183,945 - BWI76_RS00890 0.27 +0.7 183,951 - BWI76_RS00890 0.27 +0.5 184,005 - BWI76_RS00890 0.30 -0.2 184,017 + BWI76_RS00890 0.30 -0.5 184,017 + BWI76_RS00890 0.30 +0.6 184,066 + BWI76_RS00890 0.33 +1.2
Or see this region's nucleotide sequence