Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26235 and BWI76_RS26240 overlap by 1 nucleotides BWI76_RS26240 and BWI76_RS26245 are separated by 180 nucleotides BWI76_RS26245 and BWI76_RS26250 are separated by 114 nucleotides BWI76_RS26250 and BWI76_RS26255 are separated by 121 nucleotides
BWI76_RS26235: BWI76_RS26235 - gluconate transporter, at 5,431,582 to 5,432,922
_RS26235
BWI76_RS26240: BWI76_RS26240 - gluconokinase, at 5,432,922 to 5,433,452
_RS26240
BWI76_RS26245: BWI76_RS26245 - transcriptional regulator, at 5,433,633 to 5,434,628
_RS26245
BWI76_RS26250: BWI76_RS26250 - quercetin 2,3-dioxygenase, at 5,434,743 to 5,435,438
_RS26250
BWI76_RS26255: BWI76_RS26255 - oxidoreductase, at 5,435,560 to 5,436,597
_RS26255
Position (kb) 5433
5434
5435 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5432.953 kb on - strand at 5433.773 kb on - strand, within BWI76_RS26245 at 5433.900 kb on - strand, within BWI76_RS26245 at 5434.022 kb on + strand, within BWI76_RS26245 at 5434.203 kb on - strand, within BWI76_RS26245 at 5434.220 kb on + strand, within BWI76_RS26245 at 5434.826 kb on + strand, within BWI76_RS26250 at 5434.863 kb on + strand, within BWI76_RS26250 at 5434.933 kb on - strand, within BWI76_RS26250 at 5435.013 kb on + strand, within BWI76_RS26250 at 5435.019 kb on + strand, within BWI76_RS26250 at 5435.048 kb on - strand, within BWI76_RS26250 at 5435.132 kb on - strand, within BWI76_RS26250 at 5435.132 kb on - strand, within BWI76_RS26250 at 5435.142 kb on - strand, within BWI76_RS26250 at 5435.399 kb on - strand at 5435.539 kb on + strand at 5435.595 kb on + strand at 5435.595 kb on + strand at 5435.595 kb on + strand at 5435.603 kb on - strand at 5435.624 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 5,432,953 - -2.2 5,433,773 - BWI76_RS26245 0.14 -0.7 5,433,900 - BWI76_RS26245 0.27 -1.8 5,434,022 + BWI76_RS26245 0.39 -1.5 5,434,203 - BWI76_RS26245 0.57 -3.1 5,434,220 + BWI76_RS26245 0.59 -1.2 5,434,826 + BWI76_RS26250 0.12 -2.3 5,434,863 + BWI76_RS26250 0.17 -0.8 5,434,933 - BWI76_RS26250 0.27 -0.4 5,435,013 + BWI76_RS26250 0.39 -2.1 5,435,019 + BWI76_RS26250 0.40 -0.4 5,435,048 - BWI76_RS26250 0.44 -0.0 5,435,132 - BWI76_RS26250 0.56 -0.4 5,435,132 - BWI76_RS26250 0.56 +2.5 5,435,142 - BWI76_RS26250 0.57 +0.2 5,435,399 - +0.1 5,435,539 + -1.3 5,435,595 + -0.2 5,435,595 + +0.2 5,435,595 + -0.4 5,435,603 - -0.0 5,435,624 - +0.2
Or see this region's nucleotide sequence