Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11915 and BWI76_RS11920 are separated by 56 nucleotides BWI76_RS11920 and BWI76_RS11925 are separated by 410 nucleotides BWI76_RS11925 and BWI76_RS11930 are separated by 329 nucleotides
BWI76_RS11915: BWI76_RS11915 - protease SohB, at 2,506,820 to 2,507,911
_RS11915
BWI76_RS11920: BWI76_RS11920 - hypothetical protein, at 2,507,968 to 2,508,219
_RS11920
BWI76_RS11925: BWI76_RS11925 - DNA topoisomerase I, at 2,508,630 to 2,511,227
_RS11925
BWI76_RS11930: BWI76_RS11930 - LysR family transcriptional regulator CysB, at 2,511,557 to 2,512,531
_RS11930
Position (kb)
2508
2509
2510
2511
2512 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2507.666 kb on - strand, within BWI76_RS11915 at 2507.885 kb on - strand at 2507.946 kb on - strand at 2508.058 kb on - strand, within BWI76_RS11920 at 2508.082 kb on + strand, within BWI76_RS11920 at 2508.106 kb on - strand, within BWI76_RS11920 at 2508.106 kb on - strand, within BWI76_RS11920 at 2508.255 kb on - strand at 2508.383 kb on + strand at 2508.521 kb on + strand at 2511.326 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 2,507,666 - BWI76_RS11915 0.77 -0.1 2,507,885 - -1.9 2,507,946 - +0.2 2,508,058 - BWI76_RS11920 0.36 +0.1 2,508,082 + BWI76_RS11920 0.45 +0.3 2,508,106 - BWI76_RS11920 0.55 +0.1 2,508,106 - BWI76_RS11920 0.55 -0.2 2,508,255 - -2.1 2,508,383 + +0.2 2,508,521 + +0.6 2,511,326 - +3.3
Or see this region's nucleotide sequence