Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00225

Experiment: Nickel (II) chloride 1.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00220 and BWI76_RS00225 are separated by 616 nucleotidesBWI76_RS00225 and BWI76_RS00230 are separated by 112 nucleotides BWI76_RS00220: BWI76_RS00220 - F0F1 ATP synthase subunit I, at 44,948 to 45,328 _RS00220 BWI76_RS00225: BWI76_RS00225 - 16S rRNA methyltransferase G, at 45,945 to 46,568 _RS00225 BWI76_RS00230: BWI76_RS00230 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 46,681 to 48,570 _RS00230 Position (kb) 45 46 47Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 45.090 kb on - strand, within BWI76_RS00220at 45.115 kb on - strand, within BWI76_RS00220at 45.518 kb on - strandat 45.684 kb on + strandat 45.818 kb on - strandat 45.858 kb on + strandat 45.968 kb on + strandat 45.968 kb on + strandat 45.981 kb on - strandat 45.985 kb on + strandat 45.985 kb on + strandat 45.985 kb on + strandat 45.993 kb on - strandat 45.997 kb on + strandat 46.005 kb on - strandat 46.064 kb on + strand, within BWI76_RS00225at 46.064 kb on + strand, within BWI76_RS00225at 46.077 kb on - strand, within BWI76_RS00225at 46.203 kb on + strand, within BWI76_RS00225at 46.208 kb on + strand, within BWI76_RS00225at 46.211 kb on - strand, within BWI76_RS00225at 46.211 kb on - strand, within BWI76_RS00225at 46.271 kb on + strand, within BWI76_RS00225at 46.279 kb on - strand, within BWI76_RS00225at 46.290 kb on + strand, within BWI76_RS00225at 46.337 kb on + strand, within BWI76_RS00225at 46.372 kb on + strand, within BWI76_RS00225at 46.380 kb on - strand, within BWI76_RS00225at 46.427 kb on + strand, within BWI76_RS00225at 46.427 kb on + strand, within BWI76_RS00225at 46.471 kb on - strand, within BWI76_RS00225at 46.523 kb on + strandat 46.531 kb on - strandat 46.578 kb on - strandat 46.595 kb on + strandat 46.641 kb on + strandat 46.681 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM
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45,090 - BWI76_RS00220 0.37 -0.4
45,115 - BWI76_RS00220 0.44 +2.4
45,518 - -0.7
45,684 + +0.8
45,818 - +0.1
45,858 + +0.0
45,968 + -1.1
45,968 + -0.4
45,981 - +0.6
45,985 + -2.1
45,985 + -2.0
45,985 + +0.5
45,993 - -0.9
45,997 + -1.2
46,005 - -3.5
46,064 + BWI76_RS00225 0.19 -0.9
46,064 + BWI76_RS00225 0.19 +0.5
46,077 - BWI76_RS00225 0.21 +1.5
46,203 + BWI76_RS00225 0.41 -1.2
46,208 + BWI76_RS00225 0.42 -0.1
46,211 - BWI76_RS00225 0.43 -3.2
46,211 - BWI76_RS00225 0.43 -0.9
46,271 + BWI76_RS00225 0.52 -0.6
46,279 - BWI76_RS00225 0.54 +0.2
46,290 + BWI76_RS00225 0.55 -3.2
46,337 + BWI76_RS00225 0.63 -1.9
46,372 + BWI76_RS00225 0.68 -2.0
46,380 - BWI76_RS00225 0.70 -0.9
46,427 + BWI76_RS00225 0.77 -1.6
46,427 + BWI76_RS00225 0.77 -1.2
46,471 - BWI76_RS00225 0.84 -1.5
46,523 + -1.2
46,531 - -3.2
46,578 - -0.9
46,595 + -1.4
46,641 + -0.2
46,681 - -1.0

Or see this region's nucleotide sequence