Experiment: 4C survival; 24 hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_25060 and AO356_25065 overlap by 4 nucleotides AO356_25065 and AO356_25070 are separated by 179 nucleotides AO356_25070 and AO356_25075 are separated by 24 nucleotides AO356_25075 and AO356_25080 are separated by 199 nucleotides
AO356_25060: AO356_25060 - protein nirF, at 5,675,798 to 5,676,976
_25060
AO356_25065: AO356_25065 - disulfide bond formation protein DsbD, at 5,676,973 to 5,677,302
_25065
AO356_25070: AO356_25070 - cytochrome C biogenesis protein CcsA, at 5,677,482 to 5,677,790
_25070
AO356_25075: AO356_25075 - nitrite reductase, at 5,677,815 to 5,679,494
_25075
AO356_25080: AO356_25080 - AAA family ATPase, at 5,679,694 to 5,680,497
_25080
AO356_25085: AO356_25085 - cytochrome C oxidase, at 5,680,481 to 5,681,065
_25085
Position (kb)
5677
5678
5679
5680 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5677.161 kb on - strand, within AO356_25065 at 5677.323 kb on + strand at 5677.344 kb on + strand at 5677.345 kb on - strand at 5677.345 kb on - strand at 5677.442 kb on + strand at 5677.442 kb on + strand at 5677.442 kb on + strand at 5677.442 kb on + strand at 5677.442 kb on + strand at 5677.443 kb on - strand at 5677.443 kb on - strand at 5677.446 kb on + strand at 5677.446 kb on + strand at 5677.446 kb on + strand at 5677.446 kb on + strand at 5677.446 kb on + strand at 5677.447 kb on - strand at 5677.447 kb on - strand at 5677.447 kb on - strand at 5677.447 kb on - strand at 5677.487 kb on + strand at 5677.781 kb on + strand at 5677.781 kb on + strand at 5677.803 kb on + strand at 5677.819 kb on + strand at 5677.819 kb on + strand at 5677.819 kb on + strand at 5677.819 kb on + strand at 5677.820 kb on - strand at 5677.820 kb on - strand at 5677.820 kb on - strand at 5677.820 kb on - strand at 5677.840 kb on + strand at 5677.841 kb on - strand at 5677.841 kb on - strand at 5677.990 kb on + strand, within AO356_25075 at 5677.990 kb on + strand, within AO356_25075 at 5677.991 kb on - strand, within AO356_25075 at 5677.991 kb on - strand, within AO356_25075 at 5677.991 kb on - strand, within AO356_25075 at 5678.036 kb on - strand, within AO356_25075 at 5678.140 kb on + strand, within AO356_25075 at 5678.140 kb on + strand, within AO356_25075 at 5678.140 kb on + strand, within AO356_25075 at 5678.141 kb on - strand, within AO356_25075 at 5678.305 kb on + strand, within AO356_25075 at 5678.522 kb on - strand, within AO356_25075 at 5678.620 kb on + strand, within AO356_25075 at 5678.621 kb on - strand, within AO356_25075 at 5678.621 kb on - strand, within AO356_25075 at 5678.731 kb on + strand, within AO356_25075 at 5678.731 kb on + strand, within AO356_25075 at 5678.731 kb on + strand, within AO356_25075 at 5678.731 kb on + strand, within AO356_25075 at 5678.732 kb on - strand, within AO356_25075 at 5679.203 kb on - strand, within AO356_25075 at 5679.203 kb on - strand, within AO356_25075 at 5679.443 kb on + strand at 5679.443 kb on + strand at 5679.444 kb on - strand at 5679.444 kb on - strand at 5679.444 kb on - strand at 5679.444 kb on - strand at 5679.530 kb on + strand at 5679.531 kb on - strand at 5679.531 kb on - strand at 5679.531 kb on - strand at 5679.531 kb on - strand at 5679.736 kb on + strand at 5679.736 kb on + strand at 5679.736 kb on + strand at 5679.736 kb on + strand at 5679.736 kb on + strand at 5679.736 kb on + strand at 5679.737 kb on - strand at 5679.737 kb on - strand at 5679.737 kb on - strand at 5679.737 kb on - strand at 5679.874 kb on + strand, within AO356_25080 at 5679.875 kb on - strand, within AO356_25080 at 5679.875 kb on - strand, within AO356_25080 at 5679.875 kb on - strand, within AO356_25080 at 5679.875 kb on - strand, within AO356_25080 at 5680.001 kb on - strand, within AO356_25080 at 5680.078 kb on + strand, within AO356_25080 at 5680.079 kb on - strand, within AO356_25080 at 5680.170 kb on + strand, within AO356_25080
Per-strain Table
Position Strand Gene LocusTag Fraction 4C survival; 24 hrs remove 5,677,161 - AO356_25065 0.57 -0.9 5,677,323 + +0.2 5,677,344 + +0.9 5,677,345 - -0.4 5,677,345 - +0.3 5,677,442 + -0.1 5,677,442 + -0.9 5,677,442 + +0.3 5,677,442 + +0.4 5,677,442 + +0.7 5,677,443 - +0.3 5,677,443 - -0.9 5,677,446 + -0.4 5,677,446 + -0.1 5,677,446 + +0.2 5,677,446 + +0.4 5,677,446 + +0.3 5,677,447 - +1.6 5,677,447 - -1.5 5,677,447 - -0.7 5,677,447 - +0.2 5,677,487 + +0.6 5,677,781 + -0.5 5,677,781 + +0.6 5,677,803 + +0.8 5,677,819 + -1.0 5,677,819 + -0.8 5,677,819 + -0.9 5,677,819 + -1.4 5,677,820 - -0.5 5,677,820 - -0.2 5,677,820 - -2.3 5,677,820 - -0.4 5,677,840 + +2.2 5,677,841 - +0.4 5,677,841 - -0.1 5,677,990 + AO356_25075 0.10 +0.1 5,677,990 + AO356_25075 0.10 -0.6 5,677,991 - AO356_25075 0.10 -0.3 5,677,991 - AO356_25075 0.10 -0.8 5,677,991 - AO356_25075 0.10 -1.2 5,678,036 - AO356_25075 0.13 -0.3 5,678,140 + AO356_25075 0.19 -0.2 5,678,140 + AO356_25075 0.19 -0.1 5,678,140 + AO356_25075 0.19 -0.2 5,678,141 - AO356_25075 0.19 +1.1 5,678,305 + AO356_25075 0.29 +0.3 5,678,522 - AO356_25075 0.42 +0.5 5,678,620 + AO356_25075 0.48 -0.2 5,678,621 - AO356_25075 0.48 -0.4 5,678,621 - AO356_25075 0.48 -0.3 5,678,731 + AO356_25075 0.55 +0.1 5,678,731 + AO356_25075 0.55 -0.0 5,678,731 + AO356_25075 0.55 -0.3 5,678,731 + AO356_25075 0.55 -0.8 5,678,732 - AO356_25075 0.55 +0.1 5,679,203 - AO356_25075 0.83 -0.3 5,679,203 - AO356_25075 0.83 -0.9 5,679,443 + -1.0 5,679,443 + +0.5 5,679,444 - -0.9 5,679,444 - -0.0 5,679,444 - -0.9 5,679,444 - +0.0 5,679,530 + +0.0 5,679,531 - -0.1 5,679,531 - +0.5 5,679,531 - +1.5 5,679,531 - -1.4 5,679,736 + -0.6 5,679,736 + +0.3 5,679,736 + -0.8 5,679,736 + -0.1 5,679,736 + +0.9 5,679,736 + +1.1 5,679,737 - -0.2 5,679,737 - -0.1 5,679,737 - -0.4 5,679,737 - -0.2 5,679,874 + AO356_25080 0.22 +0.7 5,679,875 - AO356_25080 0.23 -0.0 5,679,875 - AO356_25080 0.23 -0.4 5,679,875 - AO356_25080 0.23 -0.7 5,679,875 - AO356_25080 0.23 -0.2 5,680,001 - AO356_25080 0.38 +0.3 5,680,078 + AO356_25080 0.48 +0.9 5,680,079 - AO356_25080 0.48 +0.3 5,680,170 + AO356_25080 0.59 -1.1
Or see this region's nucleotide sequence