Experiment: Nickel (II) chloride 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_85 and Pf6N2E2_86 are separated by 2 nucleotides Pf6N2E2_86 and Pf6N2E2_87 are separated by 570 nucleotides Pf6N2E2_87 and Pf6N2E2_88 are separated by 175 nucleotides
Pf6N2E2_85 - FIG045511: hypothetical antitoxin (to FIG022160: hypothetical toxin), at 129,986 to 130,294
_85
Pf6N2E2_86 - FIG022160: hypothetical toxin, at 130,297 to 130,467
_86
Pf6N2E2_87 - hypothetical protein, at 131,038 to 131,397
_87
Pf6N2E2_88 - hypothetical protein, at 131,573 to 131,746
_88
Position (kb) 131
132 Strain fitness (log2 ratio) -2
-1
0
1
2 at 130.061 kb on + strand, within Pf6N2E2_85 at 130.062 kb on - strand, within Pf6N2E2_85 at 130.104 kb on + strand, within Pf6N2E2_85 at 130.105 kb on - strand, within Pf6N2E2_85 at 130.105 kb on - strand, within Pf6N2E2_85 at 130.105 kb on - strand, within Pf6N2E2_85 at 130.105 kb on - strand, within Pf6N2E2_85 at 130.171 kb on - strand, within Pf6N2E2_85 at 130.227 kb on + strand, within Pf6N2E2_85 at 130.228 kb on - strand, within Pf6N2E2_85 at 130.228 kb on - strand, within Pf6N2E2_85 at 130.228 kb on - strand, within Pf6N2E2_85 at 130.228 kb on - strand, within Pf6N2E2_85 at 130.228 kb on - strand, within Pf6N2E2_85 at 130.256 kb on + strand, within Pf6N2E2_85 at 130.403 kb on + strand, within Pf6N2E2_86 at 130.403 kb on + strand, within Pf6N2E2_86 at 130.404 kb on - strand, within Pf6N2E2_86 at 130.423 kb on + strand, within Pf6N2E2_86 at 130.423 kb on + strand, within Pf6N2E2_86 at 130.424 kb on - strand, within Pf6N2E2_86 at 130.424 kb on - strand, within Pf6N2E2_86 at 130.446 kb on + strand, within Pf6N2E2_86 at 130.446 kb on + strand, within Pf6N2E2_86 at 130.447 kb on - strand, within Pf6N2E2_86 at 130.587 kb on + strand at 130.587 kb on + strand at 130.587 kb on + strand at 130.588 kb on - strand at 130.613 kb on - strand at 130.743 kb on + strand at 130.743 kb on + strand at 130.744 kb on - strand at 130.754 kb on + strand at 130.754 kb on + strand at 130.754 kb on + strand at 130.755 kb on - strand at 130.755 kb on - strand at 130.755 kb on - strand at 130.764 kb on - strand at 130.812 kb on + strand at 130.812 kb on + strand at 130.812 kb on + strand at 130.812 kb on + strand at 130.813 kb on - strand at 130.875 kb on + strand at 130.892 kb on + strand at 130.892 kb on + strand at 131.004 kb on + strand at 131.097 kb on + strand, within Pf6N2E2_87 at 131.242 kb on + strand, within Pf6N2E2_87 at 131.243 kb on - strand, within Pf6N2E2_87 at 131.284 kb on + strand, within Pf6N2E2_87 at 131.284 kb on + strand, within Pf6N2E2_87 at 131.284 kb on + strand, within Pf6N2E2_87 at 131.285 kb on - strand, within Pf6N2E2_87 at 131.285 kb on - strand, within Pf6N2E2_87 at 131.285 kb on - strand, within Pf6N2E2_87 at 131.285 kb on - strand, within Pf6N2E2_87 at 131.395 kb on + strand at 131.618 kb on + strand, within Pf6N2E2_88 at 131.687 kb on + strand, within Pf6N2E2_88 at 131.739 kb on - strand at 131.739 kb on - strand at 131.742 kb on + strand at 131.742 kb on + strand at 131.742 kb on + strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.804 kb on + strand at 131.805 kb on - strand at 132.092 kb on - strand at 132.161 kb on + strand at 132.239 kb on - strand at 132.239 kb on - strand at 132.261 kb on + strand at 132.262 kb on - strand at 132.284 kb on + strand at 132.284 kb on + strand at 132.338 kb on + strand at 132.392 kb on + strand at 132.393 kb on - strand at 132.393 kb on - strand at 132.397 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M remove 130,061 + Pf6N2E2_85 0.24 -0.8 130,062 - Pf6N2E2_85 0.25 +0.6 130,104 + Pf6N2E2_85 0.38 +1.4 130,105 - Pf6N2E2_85 0.39 +0.6 130,105 - Pf6N2E2_85 0.39 +1.2 130,105 - Pf6N2E2_85 0.39 +0.9 130,105 - Pf6N2E2_85 0.39 -0.3 130,171 - Pf6N2E2_85 0.60 +0.8 130,227 + Pf6N2E2_85 0.78 -0.2 130,228 - Pf6N2E2_85 0.78 +0.7 130,228 - Pf6N2E2_85 0.78 -0.7 130,228 - Pf6N2E2_85 0.78 -1.4 130,228 - Pf6N2E2_85 0.78 +1.9 130,228 - Pf6N2E2_85 0.78 +0.9 130,256 + Pf6N2E2_85 0.87 -1.2 130,403 + Pf6N2E2_86 0.62 -1.2 130,403 + Pf6N2E2_86 0.62 -0.7 130,404 - Pf6N2E2_86 0.63 -0.2 130,423 + Pf6N2E2_86 0.74 +0.7 130,423 + Pf6N2E2_86 0.74 -0.9 130,424 - Pf6N2E2_86 0.74 +0.2 130,424 - Pf6N2E2_86 0.74 -1.8 130,446 + Pf6N2E2_86 0.87 -0.9 130,446 + Pf6N2E2_86 0.87 -1.8 130,447 - Pf6N2E2_86 0.88 +0.7 130,587 + -0.3 130,587 + -0.6 130,587 + -0.0 130,588 - +1.3 130,613 - -0.0 130,743 + +0.1 130,743 + -1.3 130,744 - +1.0 130,754 + +0.0 130,754 + +0.3 130,754 + -1.3 130,755 - +0.6 130,755 - +1.2 130,755 - -0.2 130,764 - +0.6 130,812 + +0.4 130,812 + -2.2 130,812 + -1.5 130,812 + -0.3 130,813 - +0.2 130,875 + -1.2 130,892 + +0.1 130,892 + +0.7 131,004 + -0.9 131,097 + Pf6N2E2_87 0.16 -1.2 131,242 + Pf6N2E2_87 0.57 -1.2 131,243 - Pf6N2E2_87 0.57 -1.3 131,284 + Pf6N2E2_87 0.68 -0.2 131,284 + Pf6N2E2_87 0.68 -0.2 131,284 + Pf6N2E2_87 0.68 +0.4 131,285 - Pf6N2E2_87 0.69 -1.6 131,285 - Pf6N2E2_87 0.69 -0.7 131,285 - Pf6N2E2_87 0.69 +0.8 131,285 - Pf6N2E2_87 0.69 -1.6 131,395 + +0.6 131,618 + Pf6N2E2_88 0.26 +0.6 131,687 + Pf6N2E2_88 0.66 +1.1 131,739 - +0.7 131,739 - -2.0 131,742 + -1.0 131,742 + -0.3 131,742 + -0.4 131,743 - +1.2 131,743 - +0.5 131,743 - -1.0 131,743 - -0.0 131,743 - -0.2 131,743 - +0.2 131,804 + +0.6 131,805 - +0.3 132,092 - -0.5 132,161 + -2.2 132,239 - -0.5 132,239 - -0.2 132,261 + -0.8 132,262 - +1.2 132,284 + -0.5 132,284 + +0.7 132,338 + -0.7 132,392 + -0.0 132,393 - +0.5 132,393 - -0.4 132,397 - +0.4
Or see this region's nucleotide sequence