Experiment: Nickel (II) chloride 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_863 and Pf6N2E2_864 overlap by 4 nucleotides Pf6N2E2_864 and Pf6N2E2_865 are separated by 3 nucleotides Pf6N2E2_865 and Pf6N2E2_866 are separated by 22 nucleotides
Pf6N2E2_863 - Transcriptional regulator, IclR family, at 1,011,922 to 1,012,728
_863
Pf6N2E2_864 - 2-keto-4-pentenoate hydratase (EC 4.2.1.80), at 1,012,725 to 1,013,525
_864
Pf6N2E2_865 - Fumarylacetoacetate hydrolase family protein, at 1,013,529 to 1,014,422
_865
Pf6N2E2_866 - hypothetical protein, at 1,014,445 to 1,015,140
_866
Position (kb)
1012
1013
1014 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1011.738 kb on + strand at 1011.738 kb on + strand at 1011.829 kb on - strand at 1011.829 kb on - strand at 1011.829 kb on - strand at 1011.829 kb on - strand at 1011.831 kb on + strand at 1011.832 kb on - strand at 1011.832 kb on - strand at 1011.832 kb on - strand at 1011.907 kb on + strand at 1011.907 kb on + strand at 1011.907 kb on + strand at 1011.907 kb on + strand at 1011.908 kb on - strand at 1011.908 kb on - strand at 1011.908 kb on - strand at 1011.908 kb on - strand at 1012.151 kb on + strand, within Pf6N2E2_863 at 1012.151 kb on + strand, within Pf6N2E2_863 at 1012.151 kb on + strand, within Pf6N2E2_863 at 1012.151 kb on + strand, within Pf6N2E2_863 at 1012.151 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.293 kb on + strand, within Pf6N2E2_863 at 1012.297 kb on + strand, within Pf6N2E2_863 at 1012.504 kb on + strand, within Pf6N2E2_863 at 1012.636 kb on + strand, within Pf6N2E2_863 at 1012.636 kb on + strand, within Pf6N2E2_863 at 1012.724 kb on + strand at 1012.724 kb on + strand at 1012.725 kb on - strand at 1012.892 kb on - strand, within Pf6N2E2_864 at 1012.892 kb on - strand, within Pf6N2E2_864 at 1012.892 kb on - strand, within Pf6N2E2_864 at 1012.965 kb on + strand, within Pf6N2E2_864 at 1012.965 kb on + strand, within Pf6N2E2_864 at 1012.966 kb on - strand, within Pf6N2E2_864 at 1012.982 kb on - strand, within Pf6N2E2_864 at 1012.982 kb on - strand, within Pf6N2E2_864 at 1012.982 kb on - strand, within Pf6N2E2_864 at 1013.085 kb on + strand, within Pf6N2E2_864 at 1013.086 kb on - strand, within Pf6N2E2_864 at 1013.155 kb on - strand, within Pf6N2E2_864 at 1013.156 kb on + strand, within Pf6N2E2_864 at 1013.156 kb on + strand, within Pf6N2E2_864 at 1013.157 kb on - strand, within Pf6N2E2_864 at 1013.157 kb on - strand, within Pf6N2E2_864 at 1013.157 kb on - strand, within Pf6N2E2_864 at 1013.157 kb on - strand, within Pf6N2E2_864 at 1013.204 kb on - strand, within Pf6N2E2_864 at 1013.222 kb on - strand, within Pf6N2E2_864 at 1013.379 kb on - strand, within Pf6N2E2_864 at 1013.427 kb on + strand, within Pf6N2E2_864 at 1013.427 kb on + strand, within Pf6N2E2_864 at 1013.427 kb on + strand, within Pf6N2E2_864 at 1013.428 kb on - strand, within Pf6N2E2_864 at 1013.537 kb on - strand at 1013.537 kb on - strand at 1013.767 kb on - strand, within Pf6N2E2_865 at 1014.195 kb on - strand, within Pf6N2E2_865 at 1014.204 kb on + strand, within Pf6N2E2_865 at 1014.366 kb on + strand at 1014.366 kb on + strand at 1014.367 kb on - strand at 1014.367 kb on - strand at 1014.444 kb on + strand at 1014.468 kb on - strand at 1014.468 kb on - strand at 1014.514 kb on + strand at 1014.514 kb on + strand at 1014.515 kb on - strand, within Pf6N2E2_866 at 1014.515 kb on - strand, within Pf6N2E2_866 at 1014.515 kb on - strand, within Pf6N2E2_866 at 1014.515 kb on - strand, within Pf6N2E2_866
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M remove 1,011,738 + +0.4 1,011,738 + +0.8 1,011,829 - -0.4 1,011,829 - +0.6 1,011,829 - +0.0 1,011,829 - +0.4 1,011,831 + -1.6 1,011,832 - -0.3 1,011,832 - -0.5 1,011,832 - +0.4 1,011,907 + +1.1 1,011,907 + +0.4 1,011,907 + -0.9 1,011,907 + -1.1 1,011,908 - +0.4 1,011,908 - +0.3 1,011,908 - -0.4 1,011,908 - -0.9 1,012,151 + Pf6N2E2_863 0.28 +0.3 1,012,151 + Pf6N2E2_863 0.28 -0.6 1,012,151 + Pf6N2E2_863 0.28 +0.1 1,012,151 + Pf6N2E2_863 0.28 -1.5 1,012,151 + Pf6N2E2_863 0.28 -0.1 1,012,293 + Pf6N2E2_863 0.46 -0.6 1,012,293 + Pf6N2E2_863 0.46 -0.4 1,012,293 + Pf6N2E2_863 0.46 +0.4 1,012,293 + Pf6N2E2_863 0.46 +0.9 1,012,293 + Pf6N2E2_863 0.46 +1.4 1,012,293 + Pf6N2E2_863 0.46 -1.4 1,012,293 + Pf6N2E2_863 0.46 +1.9 1,012,293 + Pf6N2E2_863 0.46 -0.7 1,012,293 + Pf6N2E2_863 0.46 +0.6 1,012,293 + Pf6N2E2_863 0.46 +0.4 1,012,293 + Pf6N2E2_863 0.46 +0.3 1,012,293 + Pf6N2E2_863 0.46 +0.1 1,012,297 + Pf6N2E2_863 0.46 -0.6 1,012,504 + Pf6N2E2_863 0.72 -0.9 1,012,636 + Pf6N2E2_863 0.88 +1.1 1,012,636 + Pf6N2E2_863 0.88 +0.9 1,012,724 + -0.2 1,012,724 + +0.0 1,012,725 - -0.5 1,012,892 - Pf6N2E2_864 0.21 -0.1 1,012,892 - Pf6N2E2_864 0.21 +0.4 1,012,892 - Pf6N2E2_864 0.21 -0.1 1,012,965 + Pf6N2E2_864 0.30 -0.1 1,012,965 + Pf6N2E2_864 0.30 +1.4 1,012,966 - Pf6N2E2_864 0.30 -0.2 1,012,982 - Pf6N2E2_864 0.32 -0.5 1,012,982 - Pf6N2E2_864 0.32 +0.2 1,012,982 - Pf6N2E2_864 0.32 +0.0 1,013,085 + Pf6N2E2_864 0.45 +0.1 1,013,086 - Pf6N2E2_864 0.45 -0.2 1,013,155 - Pf6N2E2_864 0.54 +3.3 1,013,156 + Pf6N2E2_864 0.54 -0.9 1,013,156 + Pf6N2E2_864 0.54 +0.6 1,013,157 - Pf6N2E2_864 0.54 -1.4 1,013,157 - Pf6N2E2_864 0.54 -0.3 1,013,157 - Pf6N2E2_864 0.54 +0.1 1,013,157 - Pf6N2E2_864 0.54 -1.4 1,013,204 - Pf6N2E2_864 0.60 -0.7 1,013,222 - Pf6N2E2_864 0.62 -1.3 1,013,379 - Pf6N2E2_864 0.82 -0.4 1,013,427 + Pf6N2E2_864 0.88 -0.4 1,013,427 + Pf6N2E2_864 0.88 -1.3 1,013,427 + Pf6N2E2_864 0.88 -0.2 1,013,428 - Pf6N2E2_864 0.88 -0.2 1,013,537 - +1.7 1,013,537 - -0.4 1,013,767 - Pf6N2E2_865 0.27 +0.2 1,014,195 - Pf6N2E2_865 0.74 -1.5 1,014,204 + Pf6N2E2_865 0.76 +0.1 1,014,366 + +0.6 1,014,366 + +0.6 1,014,367 - +0.4 1,014,367 - +0.6 1,014,444 + -0.1 1,014,468 - -0.8 1,014,468 - -0.4 1,014,514 + +0.5 1,014,514 + -0.6 1,014,515 - Pf6N2E2_866 0.10 +0.4 1,014,515 - Pf6N2E2_866 0.10 +0.9 1,014,515 - Pf6N2E2_866 0.10 +0.2 1,014,515 - Pf6N2E2_866 0.10 +1.1
Or see this region's nucleotide sequence