Experiment: Nickel (II) chloride 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_433 and Pf6N2E2_434 overlap by 4 nucleotides Pf6N2E2_434 and Pf6N2E2_435 are separated by 15 nucleotides Pf6N2E2_435 and Pf6N2E2_436 overlap by 37 nucleotides
Pf6N2E2_433 - Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), at 500,337 to 501,410
_433
Pf6N2E2_434 - hypothetical protein, at 501,407 to 501,544
_434
Pf6N2E2_435 - Probable transcription regulator, at 501,560 to 502,048
_435
Pf6N2E2_436 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 502,012 to 503,085
_436
Position (kb) 501
502
503 Strain fitness (log2 ratio) -2
-1
0
1
2 at 500.677 kb on + strand, within Pf6N2E2_433 at 500.870 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433 at 501.448 kb on + strand, within Pf6N2E2_434 at 501.448 kb on + strand, within Pf6N2E2_434 at 501.525 kb on - strand, within Pf6N2E2_434 at 501.540 kb on + strand at 501.541 kb on - strand at 501.541 kb on - strand at 501.743 kb on + strand, within Pf6N2E2_435 at 501.744 kb on - strand, within Pf6N2E2_435 at 501.744 kb on - strand, within Pf6N2E2_435 at 501.744 kb on - strand, within Pf6N2E2_435 at 501.753 kb on - strand, within Pf6N2E2_435 at 501.851 kb on + strand, within Pf6N2E2_435 at 501.851 kb on + strand, within Pf6N2E2_435 at 501.920 kb on + strand, within Pf6N2E2_435 at 501.921 kb on - strand, within Pf6N2E2_435 at 501.975 kb on - strand, within Pf6N2E2_435 at 502.001 kb on + strand at 502.001 kb on + strand at 502.002 kb on - strand at 502.002 kb on - strand at 502.002 kb on - strand at 502.099 kb on + strand at 502.100 kb on - strand at 502.206 kb on - strand, within Pf6N2E2_436 at 502.300 kb on + strand, within Pf6N2E2_436 at 502.301 kb on - strand, within Pf6N2E2_436 at 502.301 kb on - strand, within Pf6N2E2_436 at 502.577 kb on + strand, within Pf6N2E2_436 at 502.578 kb on - strand, within Pf6N2E2_436 at 502.578 kb on - strand, within Pf6N2E2_436 at 502.578 kb on - strand, within Pf6N2E2_436 at 502.610 kb on + strand, within Pf6N2E2_436 at 502.610 kb on + strand, within Pf6N2E2_436 at 502.610 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.968 kb on - strand, within Pf6N2E2_436 at 502.968 kb on - strand, within Pf6N2E2_436 at 502.968 kb on - strand, within Pf6N2E2_436 at 502.984 kb on + strand at 502.991 kb on + strand at 502.991 kb on + strand at 502.992 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M remove 500,677 + Pf6N2E2_433 0.32 -0.1 500,870 - Pf6N2E2_433 0.50 -1.0 501,122 - Pf6N2E2_433 0.73 +2.1 501,122 - Pf6N2E2_433 0.73 -0.5 501,122 - Pf6N2E2_433 0.73 -0.4 501,122 - Pf6N2E2_433 0.73 -0.8 501,448 + Pf6N2E2_434 0.30 -0.2 501,448 + Pf6N2E2_434 0.30 +1.9 501,525 - Pf6N2E2_434 0.86 +0.5 501,540 + -0.7 501,541 - +0.6 501,541 - -0.3 501,743 + Pf6N2E2_435 0.37 -0.5 501,744 - Pf6N2E2_435 0.38 -1.3 501,744 - Pf6N2E2_435 0.38 -0.6 501,744 - Pf6N2E2_435 0.38 +0.2 501,753 - Pf6N2E2_435 0.39 -0.2 501,851 + Pf6N2E2_435 0.60 -0.1 501,851 + Pf6N2E2_435 0.60 -1.4 501,920 + Pf6N2E2_435 0.74 -1.0 501,921 - Pf6N2E2_435 0.74 +0.5 501,975 - Pf6N2E2_435 0.85 +0.3 502,001 + +2.1 502,001 + +1.1 502,002 - +0.5 502,002 - +0.6 502,002 - +0.1 502,099 + +0.2 502,100 - +0.4 502,206 - Pf6N2E2_436 0.18 +0.1 502,300 + Pf6N2E2_436 0.27 -1.1 502,301 - Pf6N2E2_436 0.27 -1.1 502,301 - Pf6N2E2_436 0.27 -0.2 502,577 + Pf6N2E2_436 0.53 +1.0 502,578 - Pf6N2E2_436 0.53 +0.2 502,578 - Pf6N2E2_436 0.53 +0.2 502,578 - Pf6N2E2_436 0.53 -2.0 502,610 + Pf6N2E2_436 0.56 +0.1 502,610 + Pf6N2E2_436 0.56 -0.9 502,610 + Pf6N2E2_436 0.56 -0.7 502,967 + Pf6N2E2_436 0.89 +0.6 502,967 + Pf6N2E2_436 0.89 +0.4 502,967 + Pf6N2E2_436 0.89 +0.6 502,967 + Pf6N2E2_436 0.89 -0.8 502,967 + Pf6N2E2_436 0.89 -0.1 502,967 + Pf6N2E2_436 0.89 -1.6 502,967 + Pf6N2E2_436 0.89 -0.8 502,968 - Pf6N2E2_436 0.89 -0.7 502,968 - Pf6N2E2_436 0.89 +0.0 502,968 - Pf6N2E2_436 0.89 +1.3 502,984 + -0.1 502,991 + -0.1 502,991 + -0.3 502,992 - +0.7
Or see this region's nucleotide sequence