Experiment: Nickel (II) chloride 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_252 and Pf6N2E2_253 are separated by 360 nucleotides Pf6N2E2_253 and Pf6N2E2_254 are separated by 146 nucleotides
Pf6N2E2_252 - Disease resistance protein / Tetratricopeptide repeat-containing protein, at 314,129 to 316,918
_252
Pf6N2E2_253 - Non-heme chloroperoxidase (EC 1.11.1.10), at 317,279 to 318,100
_253
Pf6N2E2_254 - Transcriptional regulator, LysR family, at 318,247 to 319,194
_254
Position (kb)
317
318
319 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 316.447 kb on + strand, within Pf6N2E2_252 at 316.635 kb on + strand, within Pf6N2E2_252 at 316.635 kb on + strand, within Pf6N2E2_252 at 316.635 kb on + strand, within Pf6N2E2_252 at 316.635 kb on + strand, within Pf6N2E2_252 at 316.702 kb on + strand at 316.909 kb on - strand at 316.909 kb on - strand at 316.909 kb on - strand at 316.964 kb on + strand at 316.964 kb on + strand at 317.013 kb on + strand at 317.013 kb on + strand at 317.014 kb on - strand at 317.122 kb on + strand at 317.123 kb on - strand at 317.214 kb on - strand at 317.247 kb on - strand at 317.294 kb on - strand at 317.315 kb on + strand at 317.315 kb on + strand at 317.315 kb on + strand at 317.316 kb on - strand at 317.316 kb on - strand at 317.316 kb on - strand at 317.316 kb on - strand at 317.318 kb on + strand at 317.318 kb on + strand at 317.319 kb on - strand at 317.742 kb on + strand, within Pf6N2E2_253 at 317.743 kb on - strand, within Pf6N2E2_253 at 317.744 kb on + strand, within Pf6N2E2_253 at 317.744 kb on + strand, within Pf6N2E2_253 at 317.744 kb on + strand, within Pf6N2E2_253 at 317.744 kb on + strand, within Pf6N2E2_253 at 317.745 kb on - strand, within Pf6N2E2_253 at 317.745 kb on - strand, within Pf6N2E2_253 at 317.745 kb on - strand, within Pf6N2E2_253 at 317.745 kb on - strand, within Pf6N2E2_253 at 317.745 kb on - strand, within Pf6N2E2_253 at 317.858 kb on + strand, within Pf6N2E2_253 at 317.858 kb on + strand, within Pf6N2E2_253 at 317.858 kb on + strand, within Pf6N2E2_253 at 317.858 kb on + strand, within Pf6N2E2_253 at 317.858 kb on + strand, within Pf6N2E2_253 at 317.858 kb on + strand, within Pf6N2E2_253 at 317.858 kb on + strand, within Pf6N2E2_253 at 317.858 kb on + strand, within Pf6N2E2_253 at 317.858 kb on + strand, within Pf6N2E2_253 at 317.858 kb on + strand, within Pf6N2E2_253 at 317.859 kb on - strand, within Pf6N2E2_253 at 317.859 kb on - strand, within Pf6N2E2_253 at 317.859 kb on - strand, within Pf6N2E2_253 at 317.859 kb on - strand, within Pf6N2E2_253 at 317.859 kb on - strand, within Pf6N2E2_253 at 317.859 kb on - strand, within Pf6N2E2_253 at 317.859 kb on - strand, within Pf6N2E2_253 at 317.859 kb on - strand, within Pf6N2E2_253 at 317.931 kb on + strand, within Pf6N2E2_253 at 318.107 kb on + strand at 318.107 kb on + strand at 318.107 kb on + strand at 318.108 kb on - strand at 318.712 kb on + strand, within Pf6N2E2_254 at 318.712 kb on + strand, within Pf6N2E2_254 at 318.817 kb on + strand, within Pf6N2E2_254 at 318.818 kb on - strand, within Pf6N2E2_254 at 318.818 kb on - strand, within Pf6N2E2_254 at 318.818 kb on - strand, within Pf6N2E2_254 at 318.818 kb on - strand, within Pf6N2E2_254 at 318.988 kb on + strand, within Pf6N2E2_254
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M remove 316,447 + Pf6N2E2_252 0.83 +0.3 316,635 + Pf6N2E2_252 0.90 -0.1 316,635 + Pf6N2E2_252 0.90 -0.9 316,635 + Pf6N2E2_252 0.90 -0.1 316,635 + Pf6N2E2_252 0.90 +3.0 316,702 + +0.0 316,909 - -0.2 316,909 - -0.1 316,909 - +0.9 316,964 + -0.1 316,964 + +1.1 317,013 + +0.9 317,013 + +0.9 317,014 - +1.1 317,122 + +0.5 317,123 - +0.7 317,214 - +0.1 317,247 - -0.1 317,294 - +0.6 317,315 + -2.0 317,315 + -0.2 317,315 + -0.5 317,316 - -1.0 317,316 - -0.3 317,316 - +0.2 317,316 - -1.2 317,318 + +0.0 317,318 + -0.4 317,319 - -0.7 317,742 + Pf6N2E2_253 0.56 -2.7 317,743 - Pf6N2E2_253 0.56 +0.2 317,744 + Pf6N2E2_253 0.57 -0.7 317,744 + Pf6N2E2_253 0.57 -0.6 317,744 + Pf6N2E2_253 0.57 -0.5 317,744 + Pf6N2E2_253 0.57 -0.5 317,745 - Pf6N2E2_253 0.57 -0.5 317,745 - Pf6N2E2_253 0.57 +0.3 317,745 - Pf6N2E2_253 0.57 -0.7 317,745 - Pf6N2E2_253 0.57 -1.8 317,745 - Pf6N2E2_253 0.57 +0.2 317,858 + Pf6N2E2_253 0.70 -0.0 317,858 + Pf6N2E2_253 0.70 -0.8 317,858 + Pf6N2E2_253 0.70 -1.9 317,858 + Pf6N2E2_253 0.70 +0.6 317,858 + Pf6N2E2_253 0.70 -0.9 317,858 + Pf6N2E2_253 0.70 -0.3 317,858 + Pf6N2E2_253 0.70 +0.9 317,858 + Pf6N2E2_253 0.70 -0.5 317,858 + Pf6N2E2_253 0.70 -0.5 317,858 + Pf6N2E2_253 0.70 +0.5 317,859 - Pf6N2E2_253 0.71 -0.3 317,859 - Pf6N2E2_253 0.71 -0.7 317,859 - Pf6N2E2_253 0.71 +0.3 317,859 - Pf6N2E2_253 0.71 -1.0 317,859 - Pf6N2E2_253 0.71 -0.5 317,859 - Pf6N2E2_253 0.71 +0.5 317,859 - Pf6N2E2_253 0.71 +0.1 317,859 - Pf6N2E2_253 0.71 +1.1 317,931 + Pf6N2E2_253 0.79 -1.2 318,107 + -1.3 318,107 + -0.0 318,107 + -1.8 318,108 - -0.1 318,712 + Pf6N2E2_254 0.49 -0.7 318,712 + Pf6N2E2_254 0.49 +0.0 318,817 + Pf6N2E2_254 0.60 -0.5 318,818 - Pf6N2E2_254 0.60 -0.5 318,818 - Pf6N2E2_254 0.60 -0.2 318,818 - Pf6N2E2_254 0.60 -0.7 318,818 - Pf6N2E2_254 0.60 -0.6 318,988 + Pf6N2E2_254 0.78 -0.6
Or see this region's nucleotide sequence