Experiment: Nickel (II) chloride 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2406 and Pf6N2E2_2407 are separated by 97 nucleotides Pf6N2E2_2407 and Pf6N2E2_2408 are separated by 180 nucleotides Pf6N2E2_2408 and Pf6N2E2_2409 are separated by 170 nucleotides
Pf6N2E2_2406 - Periplasmic binding protein, at 2,856,901 to 2,857,698
_2406
Pf6N2E2_2407 - Acyclic terpenes utilization regulator AtuR, TetR family, at 2,857,796 to 2,858,422
_2407
Pf6N2E2_2408 - 3-hydroxy-3isohexenylglutaryl-CoA:acetate lyase, at 2,858,603 to 2,860,408
_2408
Pf6N2E2_2409 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 2,860,579 to 2,862,195
_2409
Position (kb)
2858
2859
2860
2861 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2857.729 kb on + strand at 2857.730 kb on - strand at 2857.742 kb on - strand at 2857.759 kb on + strand at 2857.760 kb on - strand at 2857.781 kb on + strand at 2857.782 kb on - strand at 2857.782 kb on - strand at 2857.782 kb on - strand at 2857.782 kb on - strand at 2857.786 kb on + strand at 2857.786 kb on + strand at 2857.786 kb on + strand at 2857.786 kb on + strand at 2857.787 kb on - strand at 2857.797 kb on + strand at 2857.939 kb on + strand, within Pf6N2E2_2407 at 2857.939 kb on + strand, within Pf6N2E2_2407 at 2857.940 kb on - strand, within Pf6N2E2_2407 at 2857.940 kb on - strand, within Pf6N2E2_2407 at 2857.940 kb on - strand, within Pf6N2E2_2407 at 2857.942 kb on - strand, within Pf6N2E2_2407 at 2857.942 kb on - strand, within Pf6N2E2_2407 at 2857.989 kb on + strand, within Pf6N2E2_2407 at 2857.990 kb on - strand, within Pf6N2E2_2407 at 2857.990 kb on - strand, within Pf6N2E2_2407 at 2857.990 kb on - strand, within Pf6N2E2_2407 at 2857.994 kb on - strand, within Pf6N2E2_2407 at 2857.994 kb on - strand, within Pf6N2E2_2407 at 2858.049 kb on + strand, within Pf6N2E2_2407 at 2858.050 kb on - strand, within Pf6N2E2_2407 at 2858.050 kb on - strand, within Pf6N2E2_2407 at 2858.435 kb on - strand at 2858.495 kb on + strand at 2858.656 kb on + strand at 2858.656 kb on + strand at 2858.657 kb on - strand at 2858.696 kb on + strand at 2858.696 kb on + strand at 2858.697 kb on - strand at 2858.697 kb on - strand at 2858.711 kb on + strand at 2858.711 kb on + strand at 2858.711 kb on + strand at 2858.812 kb on - strand, within Pf6N2E2_2408 at 2859.174 kb on - strand, within Pf6N2E2_2408 at 2859.273 kb on - strand, within Pf6N2E2_2408 at 2859.413 kb on + strand, within Pf6N2E2_2408 at 2859.414 kb on - strand, within Pf6N2E2_2408 at 2859.414 kb on - strand, within Pf6N2E2_2408 at 2859.414 kb on - strand, within Pf6N2E2_2408 at 2859.414 kb on - strand, within Pf6N2E2_2408 at 2859.414 kb on - strand, within Pf6N2E2_2408 at 2859.417 kb on + strand, within Pf6N2E2_2408 at 2859.417 kb on + strand, within Pf6N2E2_2408 at 2859.417 kb on + strand, within Pf6N2E2_2408 at 2859.417 kb on + strand, within Pf6N2E2_2408 at 2859.417 kb on + strand, within Pf6N2E2_2408 at 2859.417 kb on + strand, within Pf6N2E2_2408 at 2859.417 kb on + strand, within Pf6N2E2_2408 at 2859.417 kb on + strand, within Pf6N2E2_2408 at 2859.418 kb on - strand, within Pf6N2E2_2408 at 2859.418 kb on - strand, within Pf6N2E2_2408 at 2859.418 kb on - strand, within Pf6N2E2_2408 at 2859.418 kb on - strand, within Pf6N2E2_2408 at 2859.418 kb on - strand, within Pf6N2E2_2408 at 2859.418 kb on - strand, within Pf6N2E2_2408 at 2859.521 kb on + strand, within Pf6N2E2_2408 at 2859.521 kb on + strand, within Pf6N2E2_2408 at 2859.521 kb on + strand, within Pf6N2E2_2408 at 2859.521 kb on + strand, within Pf6N2E2_2408 at 2859.521 kb on + strand, within Pf6N2E2_2408 at 2859.521 kb on + strand, within Pf6N2E2_2408 at 2859.522 kb on - strand, within Pf6N2E2_2408 at 2859.522 kb on - strand, within Pf6N2E2_2408 at 2859.522 kb on - strand, within Pf6N2E2_2408 at 2859.713 kb on + strand, within Pf6N2E2_2408 at 2859.713 kb on + strand, within Pf6N2E2_2408 at 2859.714 kb on - strand, within Pf6N2E2_2408 at 2859.714 kb on - strand, within Pf6N2E2_2408 at 2860.136 kb on + strand, within Pf6N2E2_2408 at 2860.137 kb on - strand, within Pf6N2E2_2408 at 2860.232 kb on + strand at 2860.233 kb on - strand at 2860.233 kb on - strand at 2860.874 kb on - strand, within Pf6N2E2_2409 at 2860.951 kb on + strand, within Pf6N2E2_2409 at 2860.951 kb on + strand, within Pf6N2E2_2409 at 2860.951 kb on + strand, within Pf6N2E2_2409 at 2860.951 kb on + strand, within Pf6N2E2_2409 at 2860.951 kb on + strand, within Pf6N2E2_2409 at 2860.951 kb on + strand at 2860.952 kb on - strand, within Pf6N2E2_2409 at 2860.952 kb on - strand, within Pf6N2E2_2409 at 2860.952 kb on - strand, within Pf6N2E2_2409 at 2860.952 kb on - strand, within Pf6N2E2_2409 at 2861.038 kb on + strand, within Pf6N2E2_2409 at 2861.039 kb on - strand, within Pf6N2E2_2409 at 2861.052 kb on - strand, within Pf6N2E2_2409 at 2861.052 kb on - strand, within Pf6N2E2_2409 at 2861.052 kb on - strand, within Pf6N2E2_2409 at 2861.156 kb on - strand, within Pf6N2E2_2409 at 2861.314 kb on + strand, within Pf6N2E2_2409 at 2861.314 kb on + strand, within Pf6N2E2_2409 at 2861.315 kb on - strand, within Pf6N2E2_2409
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M remove 2,857,729 + -0.1 2,857,730 - +0.7 2,857,742 - -2.0 2,857,759 + +0.8 2,857,760 - -1.5 2,857,781 + -0.1 2,857,782 - +0.2 2,857,782 - -1.2 2,857,782 - -0.6 2,857,782 - -0.2 2,857,786 + +0.3 2,857,786 + -0.3 2,857,786 + -1.6 2,857,786 + +0.5 2,857,787 - -1.0 2,857,797 + +1.5 2,857,939 + Pf6N2E2_2407 0.23 +1.5 2,857,939 + Pf6N2E2_2407 0.23 +0.1 2,857,940 - Pf6N2E2_2407 0.23 -1.6 2,857,940 - Pf6N2E2_2407 0.23 -0.8 2,857,940 - Pf6N2E2_2407 0.23 +0.2 2,857,942 - Pf6N2E2_2407 0.23 -0.3 2,857,942 - Pf6N2E2_2407 0.23 +0.3 2,857,989 + Pf6N2E2_2407 0.31 +0.4 2,857,990 - Pf6N2E2_2407 0.31 +1.7 2,857,990 - Pf6N2E2_2407 0.31 -0.1 2,857,990 - Pf6N2E2_2407 0.31 -1.0 2,857,994 - Pf6N2E2_2407 0.32 +1.0 2,857,994 - Pf6N2E2_2407 0.32 -0.8 2,858,049 + Pf6N2E2_2407 0.40 -1.6 2,858,050 - Pf6N2E2_2407 0.41 -1.0 2,858,050 - Pf6N2E2_2407 0.41 -0.6 2,858,435 - +0.4 2,858,495 + -0.8 2,858,656 + -0.0 2,858,656 + +1.5 2,858,657 - -0.3 2,858,696 + +1.4 2,858,696 + +0.3 2,858,697 - -1.7 2,858,697 - -1.2 2,858,711 + -0.4 2,858,711 + -0.0 2,858,711 + -1.7 2,858,812 - Pf6N2E2_2408 0.12 -1.0 2,859,174 - Pf6N2E2_2408 0.32 +1.3 2,859,273 - Pf6N2E2_2408 0.37 -0.2 2,859,413 + Pf6N2E2_2408 0.45 +0.9 2,859,414 - Pf6N2E2_2408 0.45 +0.6 2,859,414 - Pf6N2E2_2408 0.45 -0.3 2,859,414 - Pf6N2E2_2408 0.45 -0.0 2,859,414 - Pf6N2E2_2408 0.45 -0.7 2,859,414 - Pf6N2E2_2408 0.45 -0.3 2,859,417 + Pf6N2E2_2408 0.45 -0.6 2,859,417 + Pf6N2E2_2408 0.45 +0.7 2,859,417 + Pf6N2E2_2408 0.45 +0.3 2,859,417 + Pf6N2E2_2408 0.45 +0.3 2,859,417 + Pf6N2E2_2408 0.45 -0.7 2,859,417 + Pf6N2E2_2408 0.45 +0.7 2,859,417 + Pf6N2E2_2408 0.45 +0.0 2,859,417 + Pf6N2E2_2408 0.45 -0.1 2,859,418 - Pf6N2E2_2408 0.45 +2.0 2,859,418 - Pf6N2E2_2408 0.45 +0.3 2,859,418 - Pf6N2E2_2408 0.45 +1.5 2,859,418 - Pf6N2E2_2408 0.45 -0.9 2,859,418 - Pf6N2E2_2408 0.45 -0.7 2,859,418 - Pf6N2E2_2408 0.45 +1.6 2,859,521 + Pf6N2E2_2408 0.51 -1.8 2,859,521 + Pf6N2E2_2408 0.51 +1.4 2,859,521 + Pf6N2E2_2408 0.51 +0.9 2,859,521 + Pf6N2E2_2408 0.51 -0.0 2,859,521 + Pf6N2E2_2408 0.51 +1.4 2,859,521 + Pf6N2E2_2408 0.51 +0.6 2,859,522 - Pf6N2E2_2408 0.51 -0.1 2,859,522 - Pf6N2E2_2408 0.51 +1.3 2,859,522 - Pf6N2E2_2408 0.51 -0.1 2,859,713 + Pf6N2E2_2408 0.61 -0.8 2,859,713 + Pf6N2E2_2408 0.61 -0.6 2,859,714 - Pf6N2E2_2408 0.62 -1.5 2,859,714 - Pf6N2E2_2408 0.62 +0.7 2,860,136 + Pf6N2E2_2408 0.85 +0.8 2,860,137 - Pf6N2E2_2408 0.85 +2.3 2,860,232 + +0.9 2,860,233 - +0.0 2,860,233 - -0.4 2,860,874 - Pf6N2E2_2409 0.18 -0.9 2,860,951 + Pf6N2E2_2409 0.23 -0.4 2,860,951 + Pf6N2E2_2409 0.23 -0.3 2,860,951 + Pf6N2E2_2409 0.23 +0.5 2,860,951 + Pf6N2E2_2409 0.23 +0.9 2,860,951 + Pf6N2E2_2409 0.23 -2.3 2,860,951 + +1.3 2,860,952 - Pf6N2E2_2409 0.23 -0.3 2,860,952 - Pf6N2E2_2409 0.23 -0.6 2,860,952 - Pf6N2E2_2409 0.23 +0.0 2,860,952 - Pf6N2E2_2409 0.23 +1.3 2,861,038 + Pf6N2E2_2409 0.28 +0.3 2,861,039 - Pf6N2E2_2409 0.28 -2.2 2,861,052 - Pf6N2E2_2409 0.29 -1.2 2,861,052 - Pf6N2E2_2409 0.29 +1.2 2,861,052 - Pf6N2E2_2409 0.29 +0.1 2,861,156 - Pf6N2E2_2409 0.36 +0.8 2,861,314 + Pf6N2E2_2409 0.45 -1.5 2,861,314 + Pf6N2E2_2409 0.45 +0.5 2,861,315 - Pf6N2E2_2409 0.46 +0.7
Or see this region's nucleotide sequence