Experiment: Nickel (II) chloride 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1701 and Pf6N2E2_1702 are separated by 65 nucleotides Pf6N2E2_1702 and Pf6N2E2_1703 are separated by 105 nucleotides
Pf6N2E2_1701 - ABC transporter ATP-binding protein, at 2,009,558 to 2,010,334
_1701
Pf6N2E2_1702 - Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein, at 2,010,400 to 2,011,734
_1702
Pf6N2E2_1703 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 2,011,840 to 2,012,904
_1703
Position (kb)
2010
2011
2012 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2009.504 kb on - strand at 2009.658 kb on + strand, within Pf6N2E2_1701 at 2009.664 kb on + strand, within Pf6N2E2_1701 at 2009.665 kb on - strand, within Pf6N2E2_1701 at 2009.922 kb on + strand, within Pf6N2E2_1701 at 2009.926 kb on + strand, within Pf6N2E2_1701 at 2009.926 kb on + strand, within Pf6N2E2_1701 at 2010.401 kb on + strand at 2010.761 kb on + strand, within Pf6N2E2_1702 at 2010.761 kb on + strand, within Pf6N2E2_1702 at 2010.762 kb on - strand, within Pf6N2E2_1702 at 2010.762 kb on - strand, within Pf6N2E2_1702 at 2010.821 kb on + strand, within Pf6N2E2_1702 at 2010.822 kb on - strand, within Pf6N2E2_1702 at 2010.884 kb on + strand, within Pf6N2E2_1702 at 2010.884 kb on + strand, within Pf6N2E2_1702 at 2011.017 kb on + strand, within Pf6N2E2_1702 at 2011.018 kb on - strand, within Pf6N2E2_1702 at 2011.121 kb on + strand, within Pf6N2E2_1702 at 2011.122 kb on - strand, within Pf6N2E2_1702 at 2011.122 kb on - strand, within Pf6N2E2_1702 at 2011.241 kb on + strand, within Pf6N2E2_1702 at 2011.242 kb on - strand, within Pf6N2E2_1702 at 2011.242 kb on - strand, within Pf6N2E2_1702 at 2011.269 kb on - strand, within Pf6N2E2_1702 at 2011.269 kb on - strand, within Pf6N2E2_1702 at 2011.389 kb on - strand, within Pf6N2E2_1702 at 2011.416 kb on - strand, within Pf6N2E2_1702 at 2011.460 kb on + strand, within Pf6N2E2_1702 at 2011.460 kb on + strand, within Pf6N2E2_1702 at 2011.553 kb on + strand, within Pf6N2E2_1702 at 2011.563 kb on - strand, within Pf6N2E2_1702 at 2011.604 kb on + strand at 2011.714 kb on - strand at 2011.714 kb on - strand at 2011.714 kb on - strand at 2012.364 kb on - strand, within Pf6N2E2_1703 at 2012.438 kb on + strand, within Pf6N2E2_1703 at 2012.438 kb on + strand, within Pf6N2E2_1703 at 2012.438 kb on + strand, within Pf6N2E2_1703 at 2012.439 kb on - strand, within Pf6N2E2_1703 at 2012.439 kb on - strand, within Pf6N2E2_1703 at 2012.439 kb on - strand, within Pf6N2E2_1703 at 2012.444 kb on + strand, within Pf6N2E2_1703 at 2012.445 kb on - strand at 2012.513 kb on + strand, within Pf6N2E2_1703 at 2012.513 kb on + strand, within Pf6N2E2_1703 at 2012.514 kb on - strand, within Pf6N2E2_1703 at 2012.514 kb on - strand, within Pf6N2E2_1703 at 2012.514 kb on - strand, within Pf6N2E2_1703 at 2012.514 kb on - strand, within Pf6N2E2_1703 at 2012.634 kb on - strand, within Pf6N2E2_1703 at 2012.693 kb on + strand, within Pf6N2E2_1703
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M remove 2,009,504 - +1.4 2,009,658 + Pf6N2E2_1701 0.13 -1.7 2,009,664 + Pf6N2E2_1701 0.14 -0.3 2,009,665 - Pf6N2E2_1701 0.14 -0.3 2,009,922 + Pf6N2E2_1701 0.47 -0.9 2,009,926 + Pf6N2E2_1701 0.47 +1.0 2,009,926 + Pf6N2E2_1701 0.47 -0.0 2,010,401 + +1.0 2,010,761 + Pf6N2E2_1702 0.27 +0.6 2,010,761 + Pf6N2E2_1702 0.27 -0.1 2,010,762 - Pf6N2E2_1702 0.27 -0.3 2,010,762 - Pf6N2E2_1702 0.27 +0.2 2,010,821 + Pf6N2E2_1702 0.32 -0.1 2,010,822 - Pf6N2E2_1702 0.32 -1.1 2,010,884 + Pf6N2E2_1702 0.36 -1.3 2,010,884 + Pf6N2E2_1702 0.36 -1.4 2,011,017 + Pf6N2E2_1702 0.46 -0.0 2,011,018 - Pf6N2E2_1702 0.46 +0.3 2,011,121 + Pf6N2E2_1702 0.54 -0.7 2,011,122 - Pf6N2E2_1702 0.54 -1.0 2,011,122 - Pf6N2E2_1702 0.54 -0.6 2,011,241 + Pf6N2E2_1702 0.63 -0.6 2,011,242 - Pf6N2E2_1702 0.63 -0.4 2,011,242 - Pf6N2E2_1702 0.63 -1.5 2,011,269 - Pf6N2E2_1702 0.65 +1.0 2,011,269 - Pf6N2E2_1702 0.65 -2.0 2,011,389 - Pf6N2E2_1702 0.74 -0.4 2,011,416 - Pf6N2E2_1702 0.76 -1.8 2,011,460 + Pf6N2E2_1702 0.79 -0.5 2,011,460 + Pf6N2E2_1702 0.79 -0.1 2,011,553 + Pf6N2E2_1702 0.86 +0.5 2,011,563 - Pf6N2E2_1702 0.87 -1.6 2,011,604 + +0.0 2,011,714 - -0.4 2,011,714 - +0.7 2,011,714 - -0.1 2,012,364 - Pf6N2E2_1703 0.49 -0.4 2,012,438 + Pf6N2E2_1703 0.56 -0.8 2,012,438 + Pf6N2E2_1703 0.56 +0.8 2,012,438 + Pf6N2E2_1703 0.56 -0.0 2,012,439 - Pf6N2E2_1703 0.56 -1.6 2,012,439 - Pf6N2E2_1703 0.56 -0.6 2,012,439 - Pf6N2E2_1703 0.56 -0.4 2,012,444 + Pf6N2E2_1703 0.57 +1.2 2,012,445 - +2.0 2,012,513 + Pf6N2E2_1703 0.63 -1.5 2,012,513 + Pf6N2E2_1703 0.63 +0.7 2,012,514 - Pf6N2E2_1703 0.63 +0.4 2,012,514 - Pf6N2E2_1703 0.63 -0.7 2,012,514 - Pf6N2E2_1703 0.63 +1.0 2,012,514 - Pf6N2E2_1703 0.63 +0.1 2,012,634 - Pf6N2E2_1703 0.75 -0.0 2,012,693 + Pf6N2E2_1703 0.80 -0.7
Or see this region's nucleotide sequence