Experiment: Nickel (II) chloride 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1463 and Pf6N2E2_1464 are separated by 308 nucleotides Pf6N2E2_1464 and Pf6N2E2_1465 are separated by 99 nucleotides Pf6N2E2_1465 and Pf6N2E2_1466 are separated by 429 nucleotides Pf6N2E2_1466 and Pf6N2E2_1467 are separated by 20 nucleotides
Pf6N2E2_1463 - Isochorismate pyruvate-lyase (EC 4.-.-.-), at 1,711,658 to 1,711,936
_1463
Pf6N2E2_1464 - hypothetical protein, at 1,712,245 to 1,712,763
_1464
Pf6N2E2_1465 - hypothetical protein, at 1,712,863 to 1,714,203
_1465
Pf6N2E2_1466 - NAD-dependent formate dehydrogenase delta subunit, at 1,714,633 to 1,714,830
_1466
Pf6N2E2_1467 - NAD-dependent formate dehydrogenase alpha subunit, at 1,714,851 to 1,717,727
_1467
Position (kb)
1712
1713
1714
1715 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1712.225 kb on + strand at 1712.226 kb on - strand at 1712.265 kb on + strand at 1712.266 kb on - strand at 1712.266 kb on - strand at 1712.266 kb on - strand at 1712.527 kb on + strand, within Pf6N2E2_1464 at 1712.632 kb on + strand, within Pf6N2E2_1464 at 1712.632 kb on + strand, within Pf6N2E2_1464 at 1712.633 kb on - strand, within Pf6N2E2_1464 at 1712.633 kb on - strand, within Pf6N2E2_1464 at 1712.633 kb on - strand, within Pf6N2E2_1464 at 1712.677 kb on + strand, within Pf6N2E2_1464 at 1712.746 kb on + strand at 1712.746 kb on + strand at 1712.746 kb on + strand at 1712.747 kb on - strand at 1712.779 kb on + strand at 1712.780 kb on - strand at 1712.780 kb on - strand at 1712.937 kb on - strand at 1712.937 kb on - strand at 1712.937 kb on - strand at 1712.937 kb on - strand at 1712.937 kb on - strand at 1712.946 kb on + strand at 1712.967 kb on - strand at 1712.998 kb on - strand, within Pf6N2E2_1465 at 1713.009 kb on + strand, within Pf6N2E2_1465 at 1713.056 kb on + strand, within Pf6N2E2_1465 at 1713.066 kb on - strand, within Pf6N2E2_1465 at 1713.096 kb on + strand, within Pf6N2E2_1465 at 1713.097 kb on - strand, within Pf6N2E2_1465 at 1713.167 kb on - strand, within Pf6N2E2_1465 at 1713.543 kb on + strand, within Pf6N2E2_1465 at 1713.563 kb on + strand, within Pf6N2E2_1465 at 1713.564 kb on - strand, within Pf6N2E2_1465 at 1713.570 kb on - strand, within Pf6N2E2_1465 at 1713.570 kb on - strand, within Pf6N2E2_1465 at 1713.611 kb on + strand, within Pf6N2E2_1465 at 1713.611 kb on + strand, within Pf6N2E2_1465 at 1713.612 kb on - strand, within Pf6N2E2_1465 at 1713.645 kb on + strand, within Pf6N2E2_1465 at 1713.706 kb on - strand, within Pf6N2E2_1465 at 1713.755 kb on - strand, within Pf6N2E2_1465 at 1713.915 kb on - strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1714.000 kb on - strand, within Pf6N2E2_1465 at 1714.000 kb on - strand, within Pf6N2E2_1465 at 1714.074 kb on + strand at 1714.074 kb on + strand at 1714.074 kb on + strand at 1714.075 kb on - strand at 1714.075 kb on - strand at 1714.075 kb on - strand at 1714.075 kb on - strand at 1714.079 kb on + strand at 1714.079 kb on + strand at 1714.079 kb on + strand at 1714.080 kb on - strand at 1714.080 kb on - strand at 1714.080 kb on - strand at 1714.080 kb on - strand at 1714.094 kb on + strand at 1714.094 kb on + strand at 1714.094 kb on + strand at 1714.095 kb on - strand at 1714.448 kb on - strand at 1714.448 kb on - strand at 1714.459 kb on + strand at 1714.459 kb on + strand at 1714.459 kb on + strand at 1714.460 kb on - strand at 1714.460 kb on - strand at 1714.487 kb on - strand at 1714.611 kb on - strand at 1714.624 kb on + strand at 1714.624 kb on + strand at 1714.635 kb on - strand at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.809 kb on - strand, within Pf6N2E2_1466 at 1714.809 kb on - strand, within Pf6N2E2_1466 at 1714.809 kb on - strand, within Pf6N2E2_1466 at 1714.834 kb on + strand at 1714.987 kb on + strand at 1714.987 kb on + strand at 1714.987 kb on + strand at 1714.987 kb on + strand at 1714.988 kb on - strand at 1714.988 kb on - strand at 1715.068 kb on + strand at 1715.069 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M remove 1,712,225 + -0.2 1,712,226 - -1.3 1,712,265 + +0.7 1,712,266 - +3.1 1,712,266 - -0.3 1,712,266 - -0.8 1,712,527 + Pf6N2E2_1464 0.54 +0.1 1,712,632 + Pf6N2E2_1464 0.75 +0.1 1,712,632 + Pf6N2E2_1464 0.75 -0.3 1,712,633 - Pf6N2E2_1464 0.75 +0.9 1,712,633 - Pf6N2E2_1464 0.75 -0.8 1,712,633 - Pf6N2E2_1464 0.75 +1.1 1,712,677 + Pf6N2E2_1464 0.83 +0.5 1,712,746 + -0.6 1,712,746 + +1.4 1,712,746 + +0.2 1,712,747 - +0.5 1,712,779 + +1.5 1,712,780 - +0.8 1,712,780 - +0.2 1,712,937 - +0.8 1,712,937 - +1.6 1,712,937 - -1.4 1,712,937 - -0.9 1,712,937 - +1.1 1,712,946 + +0.5 1,712,967 - -0.3 1,712,998 - Pf6N2E2_1465 0.10 +1.1 1,713,009 + Pf6N2E2_1465 0.11 +0.2 1,713,056 + Pf6N2E2_1465 0.14 -0.8 1,713,066 - Pf6N2E2_1465 0.15 +0.5 1,713,096 + Pf6N2E2_1465 0.17 -0.4 1,713,097 - Pf6N2E2_1465 0.17 -0.2 1,713,167 - Pf6N2E2_1465 0.23 +0.0 1,713,543 + Pf6N2E2_1465 0.51 -1.2 1,713,563 + Pf6N2E2_1465 0.52 -0.8 1,713,564 - Pf6N2E2_1465 0.52 +1.2 1,713,570 - Pf6N2E2_1465 0.53 +0.9 1,713,570 - Pf6N2E2_1465 0.53 -0.3 1,713,611 + Pf6N2E2_1465 0.56 +0.1 1,713,611 + Pf6N2E2_1465 0.56 -0.1 1,713,612 - Pf6N2E2_1465 0.56 -1.0 1,713,645 + Pf6N2E2_1465 0.58 +0.5 1,713,706 - Pf6N2E2_1465 0.63 -1.2 1,713,755 - Pf6N2E2_1465 0.67 +0.9 1,713,915 - Pf6N2E2_1465 0.78 -0.6 1,713,976 + Pf6N2E2_1465 0.83 -0.8 1,713,976 + Pf6N2E2_1465 0.83 -1.0 1,713,976 + Pf6N2E2_1465 0.83 -0.8 1,713,976 + Pf6N2E2_1465 0.83 +0.2 1,714,000 - Pf6N2E2_1465 0.85 -0.9 1,714,000 - Pf6N2E2_1465 0.85 -1.1 1,714,074 + -0.6 1,714,074 + -0.1 1,714,074 + +0.4 1,714,075 - -0.1 1,714,075 - -0.7 1,714,075 - -1.8 1,714,075 - +0.9 1,714,079 + -1.2 1,714,079 + -0.2 1,714,079 + +0.7 1,714,080 - +1.2 1,714,080 - +1.1 1,714,080 - +1.8 1,714,080 - -1.4 1,714,094 + +0.1 1,714,094 + +0.2 1,714,094 + -0.9 1,714,095 - -1.3 1,714,448 - +1.8 1,714,448 - +0.8 1,714,459 + -1.2 1,714,459 + +0.2 1,714,459 + -0.8 1,714,460 - +1.2 1,714,460 - -0.2 1,714,487 - -0.6 1,714,611 - +0.5 1,714,624 + -0.8 1,714,624 + +0.0 1,714,635 - -1.2 1,714,785 + Pf6N2E2_1466 0.77 +0.1 1,714,785 + Pf6N2E2_1466 0.77 -1.3 1,714,785 + Pf6N2E2_1466 0.77 -0.5 1,714,785 + Pf6N2E2_1466 0.77 -0.2 1,714,785 + Pf6N2E2_1466 0.77 -0.7 1,714,785 + Pf6N2E2_1466 0.77 +1.1 1,714,786 - Pf6N2E2_1466 0.77 -1.0 1,714,786 - Pf6N2E2_1466 0.77 +1.8 1,714,786 - Pf6N2E2_1466 0.77 +0.3 1,714,786 - Pf6N2E2_1466 0.77 -0.6 1,714,786 - Pf6N2E2_1466 0.77 +0.3 1,714,786 - Pf6N2E2_1466 0.77 -0.4 1,714,786 - Pf6N2E2_1466 0.77 -1.3 1,714,809 - Pf6N2E2_1466 0.89 +1.0 1,714,809 - Pf6N2E2_1466 0.89 +0.2 1,714,809 - Pf6N2E2_1466 0.89 +0.5 1,714,834 + -0.2 1,714,987 + -0.3 1,714,987 + +0.8 1,714,987 + +1.1 1,714,987 + +0.2 1,714,988 - +0.8 1,714,988 - -1.1 1,715,068 + +0.8 1,715,069 - -0.6
Or see this region's nucleotide sequence