Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1459

Experiment: Nickel (II) chloride 0.0025 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1457 and Pf6N2E2_1458 are separated by 10 nucleotidesPf6N2E2_1458 and Pf6N2E2_1459 are separated by 106 nucleotidesPf6N2E2_1459 and Pf6N2E2_1460 are separated by 2 nucleotides Pf6N2E2_1457 - Xylose ABC transporter, permease protein XylH, at 1,706,003 to 1,707,139 _1457 Pf6N2E2_1458 - probable transporter, LysE family, at 1,707,150 to 1,707,758 _1458 Pf6N2E2_1459 - Glycine cleavage system transcriptional activator GcvA, at 1,707,865 to 1,708,770 _1459 Pf6N2E2_1460 - Epoxide hydrolase (EC 3.3.2.9), at 1,708,773 to 1,709,918 _1460 Position (kb) 1707 1708 1709Strain fitness (log2 ratio) -2 -1 0 1 2at 1706.987 kb on + strand, within Pf6N2E2_1457at 1706.987 kb on + strand, within Pf6N2E2_1457at 1706.988 kb on - strand, within Pf6N2E2_1457at 1706.988 kb on - strand, within Pf6N2E2_1457at 1707.060 kb on - strandat 1707.144 kb on + strandat 1707.144 kb on + strandat 1707.305 kb on + strand, within Pf6N2E2_1458at 1707.307 kb on + strand, within Pf6N2E2_1458at 1707.307 kb on + strand, within Pf6N2E2_1458at 1707.307 kb on + strand, within Pf6N2E2_1458at 1707.307 kb on + strand, within Pf6N2E2_1458at 1707.308 kb on - strand, within Pf6N2E2_1458at 1707.337 kb on + strand, within Pf6N2E2_1458at 1707.337 kb on + strand, within Pf6N2E2_1458at 1707.338 kb on - strand, within Pf6N2E2_1458at 1707.338 kb on - strand, within Pf6N2E2_1458at 1707.338 kb on - strand, within Pf6N2E2_1458at 1707.340 kb on + strandat 1707.512 kb on - strand, within Pf6N2E2_1458at 1707.536 kb on - strand, within Pf6N2E2_1458at 1707.764 kb on - strandat 1707.764 kb on - strandat 1707.845 kb on + strandat 1707.845 kb on + strandat 1707.846 kb on - strandat 1707.854 kb on - strandat 1707.986 kb on + strand, within Pf6N2E2_1459at 1707.986 kb on + strand, within Pf6N2E2_1459at 1707.987 kb on - strand, within Pf6N2E2_1459at 1707.987 kb on - strand, within Pf6N2E2_1459at 1708.292 kb on + strand, within Pf6N2E2_1459at 1708.293 kb on - strand, within Pf6N2E2_1459at 1708.293 kb on - strand, within Pf6N2E2_1459at 1708.293 kb on - strand, within Pf6N2E2_1459at 1708.361 kb on + strand, within Pf6N2E2_1459at 1708.361 kb on + strand, within Pf6N2E2_1459at 1708.361 kb on + strand, within Pf6N2E2_1459at 1708.361 kb on + strand, within Pf6N2E2_1459at 1708.362 kb on - strand, within Pf6N2E2_1459at 1708.362 kb on - strand, within Pf6N2E2_1459at 1708.362 kb on - strand, within Pf6N2E2_1459at 1708.672 kb on + strand, within Pf6N2E2_1459at 1708.673 kb on - strand, within Pf6N2E2_1459at 1708.769 kb on - strandat 1708.907 kb on - strand, within Pf6N2E2_1460at 1709.019 kb on - strand, within Pf6N2E2_1460at 1709.229 kb on + strand, within Pf6N2E2_1460at 1709.229 kb on + strand, within Pf6N2E2_1460at 1709.275 kb on - strand, within Pf6N2E2_1460at 1709.275 kb on - strand, within Pf6N2E2_1460at 1709.373 kb on + strand, within Pf6N2E2_1460at 1709.374 kb on - strand, within Pf6N2E2_1460at 1709.640 kb on + strand, within Pf6N2E2_1460at 1709.679 kb on + strand, within Pf6N2E2_1460at 1709.680 kb on - strand, within Pf6N2E2_1460

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M
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1,706,987 + Pf6N2E2_1457 0.87 +1.8
1,706,987 + Pf6N2E2_1457 0.87 -0.6
1,706,988 - Pf6N2E2_1457 0.87 +0.8
1,706,988 - Pf6N2E2_1457 0.87 -1.0
1,707,060 - +1.0
1,707,144 + -0.2
1,707,144 + -0.6
1,707,305 + Pf6N2E2_1458 0.25 +0.0
1,707,307 + Pf6N2E2_1458 0.26 +0.9
1,707,307 + Pf6N2E2_1458 0.26 +0.9
1,707,307 + Pf6N2E2_1458 0.26 +0.5
1,707,307 + Pf6N2E2_1458 0.26 -0.8
1,707,308 - Pf6N2E2_1458 0.26 -1.5
1,707,337 + Pf6N2E2_1458 0.31 +0.7
1,707,337 + Pf6N2E2_1458 0.31 +1.0
1,707,338 - Pf6N2E2_1458 0.31 +1.2
1,707,338 - Pf6N2E2_1458 0.31 +0.9
1,707,338 - Pf6N2E2_1458 0.31 +0.3
1,707,340 + +1.2
1,707,512 - Pf6N2E2_1458 0.59 +0.4
1,707,536 - Pf6N2E2_1458 0.63 +0.8
1,707,764 - -0.0
1,707,764 - -0.5
1,707,845 + -0.8
1,707,845 + -0.6
1,707,846 - +0.2
1,707,854 - -0.8
1,707,986 + Pf6N2E2_1459 0.13 -0.2
1,707,986 + Pf6N2E2_1459 0.13 +0.0
1,707,987 - Pf6N2E2_1459 0.13 -1.1
1,707,987 - Pf6N2E2_1459 0.13 +1.8
1,708,292 + Pf6N2E2_1459 0.47 -2.3
1,708,293 - Pf6N2E2_1459 0.47 +0.2
1,708,293 - Pf6N2E2_1459 0.47 -0.2
1,708,293 - Pf6N2E2_1459 0.47 -1.5
1,708,361 + Pf6N2E2_1459 0.55 -0.9
1,708,361 + Pf6N2E2_1459 0.55 -0.2
1,708,361 + Pf6N2E2_1459 0.55 -0.0
1,708,361 + Pf6N2E2_1459 0.55 +0.2
1,708,362 - Pf6N2E2_1459 0.55 -2.1
1,708,362 - Pf6N2E2_1459 0.55 -2.4
1,708,362 - Pf6N2E2_1459 0.55 -0.2
1,708,672 + Pf6N2E2_1459 0.89 -1.4
1,708,673 - Pf6N2E2_1459 0.89 -1.0
1,708,769 - +0.1
1,708,907 - Pf6N2E2_1460 0.12 +0.4
1,709,019 - Pf6N2E2_1460 0.21 +1.6
1,709,229 + Pf6N2E2_1460 0.40 -0.2
1,709,229 + Pf6N2E2_1460 0.40 +0.0
1,709,275 - Pf6N2E2_1460 0.44 -0.2
1,709,275 - Pf6N2E2_1460 0.44 +1.5
1,709,373 + Pf6N2E2_1460 0.52 +1.8
1,709,374 - Pf6N2E2_1460 0.52 +0.8
1,709,640 + Pf6N2E2_1460 0.76 +0.2
1,709,679 + Pf6N2E2_1460 0.79 +0.2
1,709,680 - Pf6N2E2_1460 0.79 -0.5

Or see this region's nucleotide sequence