Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1309

Experiment: Nickel (II) chloride 0.0025 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1307 and Pf6N2E2_1308 overlap by 4 nucleotidesPf6N2E2_1308 and Pf6N2E2_1309 are separated by 5 nucleotidesPf6N2E2_1309 and Pf6N2E2_1310 are separated by 13 nucleotides Pf6N2E2_1307 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,541,471 to 1,542,148 _1307 Pf6N2E2_1308 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,542,145 to 1,542,915 _1308 Pf6N2E2_1309 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 1,542,921 to 1,544,381 _1309 Pf6N2E2_1310 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase (EC 1.13.11.15), at 1,544,395 to 1,545,426 _1310 Position (kb) 1542 1543 1544 1545Strain fitness (log2 ratio) -1 0 1 2at 1541.976 kb on + strand, within Pf6N2E2_1307at 1541.976 kb on + strand, within Pf6N2E2_1307at 1541.976 kb on + strand, within Pf6N2E2_1307at 1541.976 kb on + strand, within Pf6N2E2_1307at 1541.976 kb on + strand, within Pf6N2E2_1307at 1542.058 kb on - strand, within Pf6N2E2_1307at 1542.112 kb on + strandat 1542.377 kb on - strand, within Pf6N2E2_1308at 1542.421 kb on + strand, within Pf6N2E2_1308at 1542.422 kb on - strand, within Pf6N2E2_1308at 1542.422 kb on - strand, within Pf6N2E2_1308at 1542.431 kb on - strand, within Pf6N2E2_1308at 1542.545 kb on - strand, within Pf6N2E2_1308at 1542.559 kb on + strand, within Pf6N2E2_1308at 1542.560 kb on - strand, within Pf6N2E2_1308at 1542.739 kb on + strand, within Pf6N2E2_1308at 1542.739 kb on + strand, within Pf6N2E2_1308at 1542.979 kb on - strandat 1543.308 kb on + strand, within Pf6N2E2_1309at 1543.318 kb on - strand, within Pf6N2E2_1309at 1543.360 kb on + strand, within Pf6N2E2_1309at 1543.361 kb on - strand, within Pf6N2E2_1309at 1543.501 kb on + strand, within Pf6N2E2_1309at 1543.565 kb on - strand, within Pf6N2E2_1309at 1543.908 kb on + strand, within Pf6N2E2_1309at 1544.136 kb on + strand, within Pf6N2E2_1309at 1544.151 kb on + strand, within Pf6N2E2_1309at 1544.152 kb on - strand, within Pf6N2E2_1309at 1544.152 kb on - strand, within Pf6N2E2_1309at 1544.301 kb on + strandat 1544.362 kb on + strandat 1544.362 kb on + strandat 1544.389 kb on + strandat 1544.390 kb on - strandat 1544.617 kb on + strand, within Pf6N2E2_1310at 1544.617 kb on + strand, within Pf6N2E2_1310at 1544.617 kb on + strand, within Pf6N2E2_1310at 1544.618 kb on - strand, within Pf6N2E2_1310at 1544.618 kb on - strand, within Pf6N2E2_1310at 1544.794 kb on + strand, within Pf6N2E2_1310at 1544.794 kb on + strand, within Pf6N2E2_1310at 1544.795 kb on - strand, within Pf6N2E2_1310at 1544.795 kb on - strand, within Pf6N2E2_1310at 1544.795 kb on - strand, within Pf6N2E2_1310at 1544.837 kb on - strand, within Pf6N2E2_1310at 1544.837 kb on - strand, within Pf6N2E2_1310at 1544.837 kb on - strand, within Pf6N2E2_1310at 1544.842 kb on + strand, within Pf6N2E2_1310at 1544.965 kb on + strand, within Pf6N2E2_1310at 1544.966 kb on - strand, within Pf6N2E2_1310at 1544.966 kb on - strand, within Pf6N2E2_1310at 1545.347 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M
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1,541,976 + Pf6N2E2_1307 0.74 +1.3
1,541,976 + Pf6N2E2_1307 0.74 -0.2
1,541,976 + Pf6N2E2_1307 0.74 +2.4
1,541,976 + Pf6N2E2_1307 0.74 -0.2
1,541,976 + Pf6N2E2_1307 0.74 -0.0
1,542,058 - Pf6N2E2_1307 0.87 +1.5
1,542,112 + +1.6
1,542,377 - Pf6N2E2_1308 0.30 +1.4
1,542,421 + Pf6N2E2_1308 0.36 +1.3
1,542,422 - Pf6N2E2_1308 0.36 -0.8
1,542,422 - Pf6N2E2_1308 0.36 +0.4
1,542,431 - Pf6N2E2_1308 0.37 -0.0
1,542,545 - Pf6N2E2_1308 0.52 -1.0
1,542,559 + Pf6N2E2_1308 0.54 -0.4
1,542,560 - Pf6N2E2_1308 0.54 -0.5
1,542,739 + Pf6N2E2_1308 0.77 +1.6
1,542,739 + Pf6N2E2_1308 0.77 +1.1
1,542,979 - -1.0
1,543,308 + Pf6N2E2_1309 0.26 -0.9
1,543,318 - Pf6N2E2_1309 0.27 -1.0
1,543,360 + Pf6N2E2_1309 0.30 -1.0
1,543,361 - Pf6N2E2_1309 0.30 -0.9
1,543,501 + Pf6N2E2_1309 0.40 +0.3
1,543,565 - Pf6N2E2_1309 0.44 +0.5
1,543,908 + Pf6N2E2_1309 0.68 -1.2
1,544,136 + Pf6N2E2_1309 0.83 -1.1
1,544,151 + Pf6N2E2_1309 0.84 -1.6
1,544,152 - Pf6N2E2_1309 0.84 -0.5
1,544,152 - Pf6N2E2_1309 0.84 +0.2
1,544,301 + +0.9
1,544,362 + -0.3
1,544,362 + -0.3
1,544,389 + -0.2
1,544,390 - -0.6
1,544,617 + Pf6N2E2_1310 0.22 -0.5
1,544,617 + Pf6N2E2_1310 0.22 -0.9
1,544,617 + Pf6N2E2_1310 0.22 +1.5
1,544,618 - Pf6N2E2_1310 0.22 +0.1
1,544,618 - Pf6N2E2_1310 0.22 +0.9
1,544,794 + Pf6N2E2_1310 0.39 -1.4
1,544,794 + Pf6N2E2_1310 0.39 +0.8
1,544,795 - Pf6N2E2_1310 0.39 +1.8
1,544,795 - Pf6N2E2_1310 0.39 -0.9
1,544,795 - Pf6N2E2_1310 0.39 +1.0
1,544,837 - Pf6N2E2_1310 0.43 +1.2
1,544,837 - Pf6N2E2_1310 0.43 +1.5
1,544,837 - Pf6N2E2_1310 0.43 +0.5
1,544,842 + Pf6N2E2_1310 0.43 -0.2
1,544,965 + Pf6N2E2_1310 0.55 -1.4
1,544,966 - Pf6N2E2_1310 0.55 -1.2
1,544,966 - Pf6N2E2_1310 0.55 +1.8
1,545,347 - +1.2

Or see this region's nucleotide sequence