Experiment: Nickel (II) chloride 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1252 and Pf6N2E2_1253 are separated by 89 nucleotides Pf6N2E2_1253 and Pf6N2E2_1254 are separated by 500 nucleotides
Pf6N2E2_1252 - Catalase (EC 1.11.1.6), at 1,465,144 to 1,466,583
_1252
Pf6N2E2_1253 - transcriptional regulator, at 1,466,673 to 1,468,034
_1253
Pf6N2E2_1254 - Cytochrome c oxidase subunit CcoN (EC 1.9.3.1), at 1,468,535 to 1,469,962
_1254
Position (kb)
1466
1467
1468
1469 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1465.803 kb on + strand, within Pf6N2E2_1252 at 1465.803 kb on + strand, within Pf6N2E2_1252 at 1466.003 kb on - strand, within Pf6N2E2_1252 at 1466.066 kb on + strand, within Pf6N2E2_1252 at 1466.066 kb on + strand, within Pf6N2E2_1252 at 1466.066 kb on + strand, within Pf6N2E2_1252 at 1466.066 kb on + strand, within Pf6N2E2_1252 at 1466.132 kb on + strand, within Pf6N2E2_1252 at 1466.132 kb on + strand, within Pf6N2E2_1252 at 1466.287 kb on + strand, within Pf6N2E2_1252 at 1466.287 kb on + strand, within Pf6N2E2_1252 at 1466.288 kb on - strand, within Pf6N2E2_1252 at 1466.288 kb on - strand, within Pf6N2E2_1252 at 1466.288 kb on - strand, within Pf6N2E2_1252 at 1466.288 kb on - strand, within Pf6N2E2_1252 at 1466.288 kb on - strand, within Pf6N2E2_1252 at 1466.288 kb on - strand, within Pf6N2E2_1252 at 1466.288 kb on - strand, within Pf6N2E2_1252 at 1466.288 kb on - strand, within Pf6N2E2_1252 at 1466.292 kb on - strand, within Pf6N2E2_1252 at 1466.353 kb on + strand, within Pf6N2E2_1252 at 1466.354 kb on - strand, within Pf6N2E2_1252 at 1466.398 kb on + strand, within Pf6N2E2_1252 at 1466.398 kb on + strand, within Pf6N2E2_1252 at 1466.399 kb on - strand, within Pf6N2E2_1252 at 1466.513 kb on + strand at 1466.513 kb on + strand at 1466.581 kb on + strand at 1466.581 kb on + strand at 1466.684 kb on + strand at 1466.684 kb on + strand at 1466.684 kb on + strand at 1466.685 kb on - strand at 1466.685 kb on - strand at 1466.770 kb on + strand at 1466.771 kb on - strand at 1466.771 kb on - strand at 1466.828 kb on - strand, within Pf6N2E2_1253 at 1467.077 kb on + strand, within Pf6N2E2_1253 at 1467.226 kb on - strand, within Pf6N2E2_1253 at 1467.226 kb on - strand, within Pf6N2E2_1253 at 1467.226 kb on - strand, within Pf6N2E2_1253 at 1467.226 kb on - strand, within Pf6N2E2_1253 at 1467.226 kb on - strand, within Pf6N2E2_1253 at 1467.333 kb on + strand, within Pf6N2E2_1253 at 1467.334 kb on - strand, within Pf6N2E2_1253 at 1467.334 kb on - strand, within Pf6N2E2_1253 at 1467.647 kb on - strand, within Pf6N2E2_1253 at 1467.647 kb on - strand, within Pf6N2E2_1253 at 1467.820 kb on - strand, within Pf6N2E2_1253 at 1467.820 kb on - strand, within Pf6N2E2_1253 at 1467.932 kb on - strand at 1468.036 kb on - strand at 1468.083 kb on + strand at 1468.084 kb on - strand at 1468.084 kb on - strand at 1468.084 kb on - strand at 1468.220 kb on - strand at 1468.222 kb on - strand at 1468.222 kb on - strand at 1468.250 kb on - strand at 1468.290 kb on - strand at 1468.290 kb on - strand at 1468.346 kb on - strand at 1468.346 kb on - strand at 1468.346 kb on - strand at 1468.399 kb on + strand at 1468.400 kb on - strand at 1468.455 kb on - strand at 1468.495 kb on + strand at 1468.495 kb on + strand at 1468.495 kb on + strand at 1468.495 kb on + strand at 1468.495 kb on + strand at 1468.495 kb on + strand at 1468.496 kb on - strand at 1468.528 kb on - strand at 1468.528 kb on - strand at 1468.531 kb on + strand at 1468.531 kb on + strand at 1468.531 kb on + strand at 1468.531 kb on + strand at 1468.532 kb on - strand at 1468.532 kb on - strand at 1468.532 kb on - strand at 1468.532 kb on - strand at 1468.532 kb on - strand at 1468.532 kb on - strand at 1468.532 kb on - strand at 1468.553 kb on - strand at 1468.562 kb on + strand at 1468.562 kb on + strand at 1468.563 kb on - strand at 1468.717 kb on + strand, within Pf6N2E2_1254 at 1468.787 kb on - strand, within Pf6N2E2_1254 at 1468.805 kb on + strand, within Pf6N2E2_1254 at 1468.904 kb on + strand, within Pf6N2E2_1254 at 1468.983 kb on + strand, within Pf6N2E2_1254 at 1468.984 kb on - strand, within Pf6N2E2_1254 at 1468.984 kb on - strand, within Pf6N2E2_1254 at 1469.009 kb on + strand, within Pf6N2E2_1254 at 1469.009 kb on + strand, within Pf6N2E2_1254 at 1469.010 kb on - strand, within Pf6N2E2_1254 at 1469.010 kb on - strand, within Pf6N2E2_1254 at 1469.010 kb on - strand, within Pf6N2E2_1254 at 1469.010 kb on - strand, within Pf6N2E2_1254 at 1469.010 kb on - strand, within Pf6N2E2_1254 at 1469.010 kb on - strand, within Pf6N2E2_1254
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M remove 1,465,803 + Pf6N2E2_1252 0.46 +1.2 1,465,803 + Pf6N2E2_1252 0.46 +0.1 1,466,003 - Pf6N2E2_1252 0.60 -0.2 1,466,066 + Pf6N2E2_1252 0.64 +0.1 1,466,066 + Pf6N2E2_1252 0.64 -0.7 1,466,066 + Pf6N2E2_1252 0.64 -0.8 1,466,066 + Pf6N2E2_1252 0.64 +0.5 1,466,132 + Pf6N2E2_1252 0.69 -2.0 1,466,132 + Pf6N2E2_1252 0.69 -0.5 1,466,287 + Pf6N2E2_1252 0.79 -1.4 1,466,287 + Pf6N2E2_1252 0.79 +1.2 1,466,288 - Pf6N2E2_1252 0.79 -1.3 1,466,288 - Pf6N2E2_1252 0.79 -1.9 1,466,288 - Pf6N2E2_1252 0.79 -0.2 1,466,288 - Pf6N2E2_1252 0.79 -0.4 1,466,288 - Pf6N2E2_1252 0.79 -0.6 1,466,288 - Pf6N2E2_1252 0.79 -0.3 1,466,288 - Pf6N2E2_1252 0.79 -1.4 1,466,288 - Pf6N2E2_1252 0.79 +0.3 1,466,292 - Pf6N2E2_1252 0.80 +0.1 1,466,353 + Pf6N2E2_1252 0.84 +0.8 1,466,354 - Pf6N2E2_1252 0.84 +0.4 1,466,398 + Pf6N2E2_1252 0.87 +0.6 1,466,398 + Pf6N2E2_1252 0.87 +0.8 1,466,399 - Pf6N2E2_1252 0.87 +1.0 1,466,513 + -0.3 1,466,513 + +0.2 1,466,581 + -0.0 1,466,581 + +0.1 1,466,684 + +0.7 1,466,684 + +0.1 1,466,684 + -0.5 1,466,685 - +1.8 1,466,685 - -0.5 1,466,770 + +0.8 1,466,771 - -0.4 1,466,771 - +0.9 1,466,828 - Pf6N2E2_1253 0.11 +1.2 1,467,077 + Pf6N2E2_1253 0.30 +1.8 1,467,226 - Pf6N2E2_1253 0.41 -1.0 1,467,226 - Pf6N2E2_1253 0.41 +0.3 1,467,226 - Pf6N2E2_1253 0.41 +0.2 1,467,226 - Pf6N2E2_1253 0.41 -0.2 1,467,226 - Pf6N2E2_1253 0.41 +0.9 1,467,333 + Pf6N2E2_1253 0.48 +1.0 1,467,334 - Pf6N2E2_1253 0.49 +0.9 1,467,334 - Pf6N2E2_1253 0.49 -0.0 1,467,647 - Pf6N2E2_1253 0.72 -0.0 1,467,647 - Pf6N2E2_1253 0.72 -0.1 1,467,820 - Pf6N2E2_1253 0.84 -0.1 1,467,820 - Pf6N2E2_1253 0.84 +0.8 1,467,932 - +0.4 1,468,036 - -0.4 1,468,083 + -0.8 1,468,084 - +1.4 1,468,084 - +0.8 1,468,084 - +0.5 1,468,220 - +0.1 1,468,222 - -1.0 1,468,222 - -0.2 1,468,250 - -1.0 1,468,290 - -0.0 1,468,290 - +1.2 1,468,346 - +2.2 1,468,346 - +0.2 1,468,346 - -0.2 1,468,399 + -1.1 1,468,400 - -2.3 1,468,455 - -0.5 1,468,495 + -1.7 1,468,495 + -0.8 1,468,495 + -1.1 1,468,495 + +1.1 1,468,495 + -0.2 1,468,495 + -0.8 1,468,496 - +1.4 1,468,528 - +0.7 1,468,528 - +2.8 1,468,531 + +2.4 1,468,531 + -0.0 1,468,531 + +1.4 1,468,531 + +1.3 1,468,532 - +0.2 1,468,532 - -0.5 1,468,532 - +0.6 1,468,532 - -0.1 1,468,532 - -0.5 1,468,532 - -0.2 1,468,532 - -0.2 1,468,553 - -1.2 1,468,562 + +0.5 1,468,562 + +2.5 1,468,563 - -0.3 1,468,717 + Pf6N2E2_1254 0.13 +1.2 1,468,787 - Pf6N2E2_1254 0.18 +1.5 1,468,805 + Pf6N2E2_1254 0.19 -1.4 1,468,904 + Pf6N2E2_1254 0.26 -0.2 1,468,983 + Pf6N2E2_1254 0.31 +0.8 1,468,984 - Pf6N2E2_1254 0.31 +1.1 1,468,984 - Pf6N2E2_1254 0.31 -0.2 1,469,009 + Pf6N2E2_1254 0.33 +0.2 1,469,009 + Pf6N2E2_1254 0.33 +0.1 1,469,010 - Pf6N2E2_1254 0.33 +1.0 1,469,010 - Pf6N2E2_1254 0.33 +0.8 1,469,010 - Pf6N2E2_1254 0.33 -1.5 1,469,010 - Pf6N2E2_1254 0.33 +0.5 1,469,010 - Pf6N2E2_1254 0.33 +0.5 1,469,010 - Pf6N2E2_1254 0.33 +0.8
Or see this region's nucleotide sequence