Experiment: Nickel (II) chloride 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1229 and Pf6N2E2_1230 are separated by 68 nucleotides Pf6N2E2_1230 and Pf6N2E2_1231 overlap by 7 nucleotides Pf6N2E2_1231 and Pf6N2E2_1232 are separated by 14 nucleotides
Pf6N2E2_1229 - Serine protease homologue, at 1,438,735 to 1,441,827
_1229
Pf6N2E2_1230 - Ferric iron ABC transporter, permease protein, at 1,441,896 to 1,442,084
_1230
Pf6N2E2_1231 - ABC-type protease exporter, outer membrane component PrtF/AprF, at 1,442,078 to 1,443,427
_1231
Pf6N2E2_1232 - ABC-type protease exporter, membrane fusion protein (MFP) family component PrtE/AprE, at 1,443,442 to 1,444,758
_1232
Position (kb)
1442
1443
1444 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1441.250 kb on + strand, within Pf6N2E2_1229 at 1441.250 kb on + strand, within Pf6N2E2_1229 at 1441.250 kb on + strand, within Pf6N2E2_1229 at 1441.250 kb on + strand, within Pf6N2E2_1229 at 1441.251 kb on - strand, within Pf6N2E2_1229 at 1441.332 kb on - strand, within Pf6N2E2_1229 at 1441.332 kb on - strand, within Pf6N2E2_1229 at 1441.475 kb on + strand, within Pf6N2E2_1229 at 1441.475 kb on + strand, within Pf6N2E2_1229 at 1441.475 kb on + strand, within Pf6N2E2_1229 at 1441.475 kb on + strand, within Pf6N2E2_1229 at 1441.475 kb on + strand, within Pf6N2E2_1229 at 1441.475 kb on + strand, within Pf6N2E2_1229 at 1441.475 kb on + strand, within Pf6N2E2_1229 at 1441.476 kb on - strand, within Pf6N2E2_1229 at 1441.487 kb on + strand, within Pf6N2E2_1229 at 1441.614 kb on - strand at 1441.710 kb on - strand at 1441.710 kb on - strand at 1441.841 kb on + strand at 1441.841 kb on + strand at 1441.842 kb on - strand at 1441.842 kb on - strand at 1441.842 kb on - strand at 1441.842 kb on - strand at 1441.842 kb on - strand at 1441.842 kb on - strand at 1441.842 kb on - strand at 1442.151 kb on + strand at 1442.152 kb on - strand at 1442.152 kb on - strand at 1442.154 kb on + strand at 1442.182 kb on - strand at 1442.220 kb on + strand, within Pf6N2E2_1231 at 1442.329 kb on - strand, within Pf6N2E2_1231 at 1442.435 kb on - strand, within Pf6N2E2_1231 at 1442.482 kb on - strand, within Pf6N2E2_1231 at 1442.482 kb on - strand, within Pf6N2E2_1231 at 1442.482 kb on - strand, within Pf6N2E2_1231 at 1442.482 kb on - strand, within Pf6N2E2_1231 at 1442.536 kb on - strand, within Pf6N2E2_1231 at 1442.536 kb on - strand, within Pf6N2E2_1231 at 1442.539 kb on + strand, within Pf6N2E2_1231 at 1442.539 kb on + strand, within Pf6N2E2_1231 at 1442.540 kb on - strand, within Pf6N2E2_1231 at 1442.540 kb on - strand, within Pf6N2E2_1231 at 1442.540 kb on - strand, within Pf6N2E2_1231 at 1442.540 kb on - strand, within Pf6N2E2_1231 at 1442.859 kb on + strand, within Pf6N2E2_1231 at 1442.893 kb on + strand, within Pf6N2E2_1231 at 1443.003 kb on + strand, within Pf6N2E2_1231 at 1443.004 kb on - strand, within Pf6N2E2_1231 at 1443.108 kb on + strand, within Pf6N2E2_1231 at 1443.109 kb on - strand, within Pf6N2E2_1231 at 1443.147 kb on + strand, within Pf6N2E2_1231 at 1443.156 kb on + strand, within Pf6N2E2_1231 at 1443.156 kb on + strand, within Pf6N2E2_1231 at 1443.157 kb on - strand, within Pf6N2E2_1231 at 1443.157 kb on - strand, within Pf6N2E2_1231 at 1443.199 kb on - strand, within Pf6N2E2_1231 at 1443.231 kb on + strand, within Pf6N2E2_1231 at 1443.232 kb on - strand, within Pf6N2E2_1231 at 1443.237 kb on + strand, within Pf6N2E2_1231 at 1443.443 kb on + strand at 1443.443 kb on + strand at 1443.444 kb on - strand at 1443.494 kb on + strand at 1443.495 kb on - strand at 1443.495 kb on - strand at 1443.611 kb on + strand, within Pf6N2E2_1232 at 1443.611 kb on + strand, within Pf6N2E2_1232 at 1443.611 kb on + strand, within Pf6N2E2_1232 at 1443.611 kb on + strand, within Pf6N2E2_1232 at 1443.611 kb on + strand, within Pf6N2E2_1232 at 1443.612 kb on - strand, within Pf6N2E2_1232 at 1443.612 kb on - strand, within Pf6N2E2_1232 at 1443.612 kb on - strand, within Pf6N2E2_1232 at 1443.612 kb on - strand, within Pf6N2E2_1232 at 1443.612 kb on - strand, within Pf6N2E2_1232 at 1443.612 kb on - strand, within Pf6N2E2_1232 at 1443.614 kb on + strand, within Pf6N2E2_1232 at 1443.992 kb on + strand, within Pf6N2E2_1232 at 1444.136 kb on + strand, within Pf6N2E2_1232 at 1444.136 kb on + strand, within Pf6N2E2_1232 at 1444.136 kb on + strand, within Pf6N2E2_1232 at 1444.137 kb on - strand, within Pf6N2E2_1232 at 1444.137 kb on - strand, within Pf6N2E2_1232 at 1444.137 kb on - strand, within Pf6N2E2_1232 at 1444.381 kb on + strand, within Pf6N2E2_1232 at 1444.381 kb on + strand, within Pf6N2E2_1232 at 1444.381 kb on + strand, within Pf6N2E2_1232 at 1444.382 kb on - strand, within Pf6N2E2_1232 at 1444.382 kb on - strand, within Pf6N2E2_1232
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M remove 1,441,250 + Pf6N2E2_1229 0.81 -0.8 1,441,250 + Pf6N2E2_1229 0.81 +0.2 1,441,250 + Pf6N2E2_1229 0.81 -1.5 1,441,250 + Pf6N2E2_1229 0.81 -1.5 1,441,251 - Pf6N2E2_1229 0.81 +1.8 1,441,332 - Pf6N2E2_1229 0.84 -1.4 1,441,332 - Pf6N2E2_1229 0.84 +0.7 1,441,475 + Pf6N2E2_1229 0.89 +0.9 1,441,475 + Pf6N2E2_1229 0.89 +0.1 1,441,475 + Pf6N2E2_1229 0.89 +1.1 1,441,475 + Pf6N2E2_1229 0.89 -2.3 1,441,475 + Pf6N2E2_1229 0.89 -1.0 1,441,475 + Pf6N2E2_1229 0.89 -0.2 1,441,475 + Pf6N2E2_1229 0.89 -0.2 1,441,476 - Pf6N2E2_1229 0.89 -0.8 1,441,487 + Pf6N2E2_1229 0.89 -0.6 1,441,614 - +0.1 1,441,710 - -2.3 1,441,710 - -1.0 1,441,841 + -0.5 1,441,841 + +0.5 1,441,842 - -1.9 1,441,842 - +0.5 1,441,842 - -0.2 1,441,842 - -0.2 1,441,842 - -0.6 1,441,842 - -0.6 1,441,842 - -2.3 1,442,151 + +0.1 1,442,152 - +0.6 1,442,152 - -0.5 1,442,154 + -0.0 1,442,182 - -1.3 1,442,220 + Pf6N2E2_1231 0.11 +1.8 1,442,329 - Pf6N2E2_1231 0.19 +1.8 1,442,435 - Pf6N2E2_1231 0.26 -1.8 1,442,482 - Pf6N2E2_1231 0.30 +1.8 1,442,482 - Pf6N2E2_1231 0.30 +0.6 1,442,482 - Pf6N2E2_1231 0.30 +0.8 1,442,482 - Pf6N2E2_1231 0.30 +0.5 1,442,536 - Pf6N2E2_1231 0.34 +0.7 1,442,536 - Pf6N2E2_1231 0.34 -0.9 1,442,539 + Pf6N2E2_1231 0.34 +1.5 1,442,539 + Pf6N2E2_1231 0.34 +0.4 1,442,540 - Pf6N2E2_1231 0.34 +1.6 1,442,540 - Pf6N2E2_1231 0.34 -0.0 1,442,540 - Pf6N2E2_1231 0.34 +0.6 1,442,540 - Pf6N2E2_1231 0.34 -0.8 1,442,859 + Pf6N2E2_1231 0.58 +0.6 1,442,893 + Pf6N2E2_1231 0.60 +1.8 1,443,003 + Pf6N2E2_1231 0.69 +2.4 1,443,004 - Pf6N2E2_1231 0.69 -0.5 1,443,108 + Pf6N2E2_1231 0.76 -0.5 1,443,109 - Pf6N2E2_1231 0.76 +0.6 1,443,147 + Pf6N2E2_1231 0.79 -0.1 1,443,156 + Pf6N2E2_1231 0.80 -1.0 1,443,156 + Pf6N2E2_1231 0.80 +1.2 1,443,157 - Pf6N2E2_1231 0.80 -0.2 1,443,157 - Pf6N2E2_1231 0.80 +0.6 1,443,199 - Pf6N2E2_1231 0.83 -0.2 1,443,231 + Pf6N2E2_1231 0.85 +0.2 1,443,232 - Pf6N2E2_1231 0.85 +0.5 1,443,237 + Pf6N2E2_1231 0.86 -1.4 1,443,443 + -0.1 1,443,443 + +0.2 1,443,444 - +0.3 1,443,494 + +1.4 1,443,495 - +1.2 1,443,495 - -1.7 1,443,611 + Pf6N2E2_1232 0.13 -0.4 1,443,611 + Pf6N2E2_1232 0.13 -0.3 1,443,611 + Pf6N2E2_1232 0.13 -0.2 1,443,611 + Pf6N2E2_1232 0.13 -2.2 1,443,611 + Pf6N2E2_1232 0.13 +1.8 1,443,612 - Pf6N2E2_1232 0.13 -0.9 1,443,612 - Pf6N2E2_1232 0.13 +0.9 1,443,612 - Pf6N2E2_1232 0.13 -0.5 1,443,612 - Pf6N2E2_1232 0.13 +0.2 1,443,612 - Pf6N2E2_1232 0.13 -1.5 1,443,612 - Pf6N2E2_1232 0.13 +0.2 1,443,614 + Pf6N2E2_1232 0.13 +2.2 1,443,992 + Pf6N2E2_1232 0.42 +0.8 1,444,136 + Pf6N2E2_1232 0.53 -0.2 1,444,136 + Pf6N2E2_1232 0.53 +0.5 1,444,136 + Pf6N2E2_1232 0.53 +0.5 1,444,137 - Pf6N2E2_1232 0.53 -1.1 1,444,137 - Pf6N2E2_1232 0.53 -0.4 1,444,137 - Pf6N2E2_1232 0.53 -1.2 1,444,381 + Pf6N2E2_1232 0.71 +0.5 1,444,381 + Pf6N2E2_1232 0.71 -0.9 1,444,381 + Pf6N2E2_1232 0.71 +0.7 1,444,382 - Pf6N2E2_1232 0.71 -0.8 1,444,382 - Pf6N2E2_1232 0.71 +0.2
Or see this region's nucleotide sequence