Experiment: Nickel (II) chloride 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1229 and Pf6N2E2_1230 are separated by 68 nucleotides Pf6N2E2_1230 and Pf6N2E2_1231 overlap by 7 nucleotides
Pf6N2E2_1229 - Serine protease homologue, at 1,438,735 to 1,441,827
_1229
Pf6N2E2_1230 - Ferric iron ABC transporter, permease protein, at 1,441,896 to 1,442,084
_1230
Pf6N2E2_1231 - ABC-type protease exporter, outer membrane component PrtF/AprF, at 1,442,078 to 1,443,427
_1231
Position (kb)
1441
1442
1443 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1440.959 kb on + strand, within Pf6N2E2_1229 at 1440.960 kb on - strand, within Pf6N2E2_1229 at 1440.998 kb on + strand, within Pf6N2E2_1229 at 1440.998 kb on + strand, within Pf6N2E2_1229 at 1440.999 kb on - strand, within Pf6N2E2_1229 at 1441.250 kb on + strand, within Pf6N2E2_1229 at 1441.250 kb on + strand, within Pf6N2E2_1229 at 1441.250 kb on + strand, within Pf6N2E2_1229 at 1441.250 kb on + strand, within Pf6N2E2_1229 at 1441.251 kb on - strand, within Pf6N2E2_1229 at 1441.332 kb on - strand, within Pf6N2E2_1229 at 1441.332 kb on - strand, within Pf6N2E2_1229 at 1441.475 kb on + strand, within Pf6N2E2_1229 at 1441.475 kb on + strand, within Pf6N2E2_1229 at 1441.475 kb on + strand, within Pf6N2E2_1229 at 1441.475 kb on + strand, within Pf6N2E2_1229 at 1441.475 kb on + strand, within Pf6N2E2_1229 at 1441.475 kb on + strand, within Pf6N2E2_1229 at 1441.475 kb on + strand, within Pf6N2E2_1229 at 1441.476 kb on - strand, within Pf6N2E2_1229 at 1441.487 kb on + strand, within Pf6N2E2_1229 at 1441.614 kb on - strand at 1441.710 kb on - strand at 1441.710 kb on - strand at 1441.841 kb on + strand at 1441.841 kb on + strand at 1441.842 kb on - strand at 1441.842 kb on - strand at 1441.842 kb on - strand at 1441.842 kb on - strand at 1441.842 kb on - strand at 1441.842 kb on - strand at 1441.842 kb on - strand at 1442.151 kb on + strand at 1442.152 kb on - strand at 1442.152 kb on - strand at 1442.154 kb on + strand at 1442.182 kb on - strand at 1442.220 kb on + strand, within Pf6N2E2_1231 at 1442.329 kb on - strand, within Pf6N2E2_1231 at 1442.435 kb on - strand, within Pf6N2E2_1231 at 1442.482 kb on - strand, within Pf6N2E2_1231 at 1442.482 kb on - strand, within Pf6N2E2_1231 at 1442.482 kb on - strand, within Pf6N2E2_1231 at 1442.482 kb on - strand, within Pf6N2E2_1231 at 1442.536 kb on - strand, within Pf6N2E2_1231 at 1442.536 kb on - strand, within Pf6N2E2_1231 at 1442.539 kb on + strand, within Pf6N2E2_1231 at 1442.539 kb on + strand, within Pf6N2E2_1231 at 1442.540 kb on - strand, within Pf6N2E2_1231 at 1442.540 kb on - strand, within Pf6N2E2_1231 at 1442.540 kb on - strand, within Pf6N2E2_1231 at 1442.540 kb on - strand, within Pf6N2E2_1231 at 1442.859 kb on + strand, within Pf6N2E2_1231 at 1442.893 kb on + strand, within Pf6N2E2_1231 at 1443.003 kb on + strand, within Pf6N2E2_1231 at 1443.004 kb on - strand, within Pf6N2E2_1231
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M remove 1,440,959 + Pf6N2E2_1229 0.72 -0.2 1,440,960 - Pf6N2E2_1229 0.72 +0.2 1,440,998 + Pf6N2E2_1229 0.73 +0.4 1,440,998 + Pf6N2E2_1229 0.73 +1.5 1,440,999 - Pf6N2E2_1229 0.73 +0.2 1,441,250 + Pf6N2E2_1229 0.81 -0.8 1,441,250 + Pf6N2E2_1229 0.81 +0.2 1,441,250 + Pf6N2E2_1229 0.81 -1.5 1,441,250 + Pf6N2E2_1229 0.81 -1.5 1,441,251 - Pf6N2E2_1229 0.81 +1.8 1,441,332 - Pf6N2E2_1229 0.84 -1.4 1,441,332 - Pf6N2E2_1229 0.84 +0.7 1,441,475 + Pf6N2E2_1229 0.89 +0.9 1,441,475 + Pf6N2E2_1229 0.89 +0.1 1,441,475 + Pf6N2E2_1229 0.89 +1.1 1,441,475 + Pf6N2E2_1229 0.89 -2.3 1,441,475 + Pf6N2E2_1229 0.89 -1.0 1,441,475 + Pf6N2E2_1229 0.89 -0.2 1,441,475 + Pf6N2E2_1229 0.89 -0.2 1,441,476 - Pf6N2E2_1229 0.89 -0.8 1,441,487 + Pf6N2E2_1229 0.89 -0.6 1,441,614 - +0.1 1,441,710 - -2.3 1,441,710 - -1.0 1,441,841 + -0.5 1,441,841 + +0.5 1,441,842 - -1.9 1,441,842 - +0.5 1,441,842 - -0.2 1,441,842 - -0.2 1,441,842 - -0.6 1,441,842 - -0.6 1,441,842 - -2.3 1,442,151 + +0.1 1,442,152 - +0.6 1,442,152 - -0.5 1,442,154 + -0.0 1,442,182 - -1.3 1,442,220 + Pf6N2E2_1231 0.11 +1.8 1,442,329 - Pf6N2E2_1231 0.19 +1.8 1,442,435 - Pf6N2E2_1231 0.26 -1.8 1,442,482 - Pf6N2E2_1231 0.30 +1.8 1,442,482 - Pf6N2E2_1231 0.30 +0.6 1,442,482 - Pf6N2E2_1231 0.30 +0.8 1,442,482 - Pf6N2E2_1231 0.30 +0.5 1,442,536 - Pf6N2E2_1231 0.34 +0.7 1,442,536 - Pf6N2E2_1231 0.34 -0.9 1,442,539 + Pf6N2E2_1231 0.34 +1.5 1,442,539 + Pf6N2E2_1231 0.34 +0.4 1,442,540 - Pf6N2E2_1231 0.34 +1.6 1,442,540 - Pf6N2E2_1231 0.34 -0.0 1,442,540 - Pf6N2E2_1231 0.34 +0.6 1,442,540 - Pf6N2E2_1231 0.34 -0.8 1,442,859 + Pf6N2E2_1231 0.58 +0.6 1,442,893 + Pf6N2E2_1231 0.60 +1.8 1,443,003 + Pf6N2E2_1231 0.69 +2.4 1,443,004 - Pf6N2E2_1231 0.69 -0.5
Or see this region's nucleotide sequence