Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27595 and BWI76_RS27600 are separated by 291 nucleotides BWI76_RS27600 and BWI76_RS27605 are separated by 38 nucleotides BWI76_RS27605 and BWI76_RS27610 are separated by 326 nucleotides BWI76_RS27610 and BWI76_RS27615 are separated by 95 nucleotides BWI76_RS27615 and BWI76_RS27620 are separated by 185 nucleotides
BWI76_RS27595: BWI76_RS27595 - glycoside-pentoside-hexuronide family transporter, at 5,728,796 to 5,730,190
_RS27595
BWI76_RS27600: BWI76_RS27600 - tRNA-Sec, at 5,730,482 to 5,730,576
_RS27600
BWI76_RS27605: BWI76_RS27605 - hypothetical protein, at 5,730,615 to 5,730,806
_RS27605
BWI76_RS27610: BWI76_RS27610 - hydroxyacid dehydrogenase, at 5,731,133 to 5,731,647
_RS27610
BWI76_RS27615: BWI76_RS27615 - putative Shikimate kinase, at 5,731,743 to 5,732,348
_RS27615
BWI76_RS27620: BWI76_RS27620 - hypothetical protein, at 5,732,534 to 5,733,001
_RS27620
Position (kb)
5731
5732 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5730.549 kb on - strand, within BWI76_RS27600 at 5730.559 kb on - strand, within BWI76_RS27600 at 5730.559 kb on - strand, within BWI76_RS27600 at 5730.602 kb on - strand at 5730.673 kb on - strand, within BWI76_RS27605 at 5730.689 kb on + strand, within BWI76_RS27605 at 5730.856 kb on + strand at 5730.866 kb on + strand at 5730.987 kb on - strand at 5731.123 kb on - strand at 5731.161 kb on + strand at 5731.265 kb on + strand, within BWI76_RS27610 at 5731.273 kb on - strand, within BWI76_RS27610 at 5731.298 kb on + strand, within BWI76_RS27610 at 5731.576 kb on - strand, within BWI76_RS27610 at 5731.627 kb on - strand at 5731.627 kb on - strand at 5731.650 kb on - strand at 5731.710 kb on + strand at 5731.718 kb on - strand at 5731.718 kb on - strand at 5731.727 kb on - strand at 5731.727 kb on - strand at 5731.852 kb on + strand, within BWI76_RS27615 at 5731.952 kb on + strand, within BWI76_RS27615 at 5731.952 kb on + strand, within BWI76_RS27615 at 5731.952 kb on + strand, within BWI76_RS27615 at 5731.960 kb on - strand, within BWI76_RS27615 at 5731.960 kb on - strand, within BWI76_RS27615 at 5731.960 kb on - strand, within BWI76_RS27615 at 5731.960 kb on - strand, within BWI76_RS27615 at 5731.989 kb on - strand, within BWI76_RS27615 at 5732.019 kb on + strand, within BWI76_RS27615 at 5732.153 kb on + strand, within BWI76_RS27615 at 5732.178 kb on - strand, within BWI76_RS27615 at 5732.280 kb on - strand, within BWI76_RS27615 at 5732.294 kb on + strand at 5732.348 kb on + strand at 5732.356 kb on - strand at 5732.356 kb on - strand at 5732.356 kb on - strand at 5732.356 kb on - strand at 5732.374 kb on + strand at 5732.432 kb on + strand at 5732.440 kb on - strand at 5732.460 kb on - strand at 5732.492 kb on - strand at 5732.568 kb on + strand at 5732.576 kb on - strand at 5732.576 kb on - strand at 5732.587 kb on + strand, within BWI76_RS27620 at 5732.624 kb on - strand, within BWI76_RS27620 at 5732.631 kb on + strand, within BWI76_RS27620 at 5732.639 kb on - strand, within BWI76_RS27620 at 5732.639 kb on - strand, within BWI76_RS27620
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 5,730,549 - BWI76_RS27600 0.71 -0.6 5,730,559 - BWI76_RS27600 0.81 -0.9 5,730,559 - BWI76_RS27600 0.81 -0.6 5,730,602 - -0.8 5,730,673 - BWI76_RS27605 0.30 -0.1 5,730,689 + BWI76_RS27605 0.39 -0.5 5,730,856 + +0.2 5,730,866 + -0.4 5,730,987 - +0.6 5,731,123 - -0.8 5,731,161 + -0.7 5,731,265 + BWI76_RS27610 0.26 +0.5 5,731,273 - BWI76_RS27610 0.27 +1.8 5,731,298 + BWI76_RS27610 0.32 +0.5 5,731,576 - BWI76_RS27610 0.86 +0.5 5,731,627 - +0.3 5,731,627 - +0.3 5,731,650 - -0.3 5,731,710 + +0.3 5,731,718 - -0.3 5,731,718 - -1.7 5,731,727 - +0.1 5,731,727 - +0.2 5,731,852 + BWI76_RS27615 0.18 -1.0 5,731,952 + BWI76_RS27615 0.34 -0.4 5,731,952 + BWI76_RS27615 0.34 -0.3 5,731,952 + BWI76_RS27615 0.34 +0.2 5,731,960 - BWI76_RS27615 0.36 +0.0 5,731,960 - BWI76_RS27615 0.36 -0.1 5,731,960 - BWI76_RS27615 0.36 +0.7 5,731,960 - BWI76_RS27615 0.36 -0.3 5,731,989 - BWI76_RS27615 0.41 +0.1 5,732,019 + BWI76_RS27615 0.46 -1.2 5,732,153 + BWI76_RS27615 0.68 +0.5 5,732,178 - BWI76_RS27615 0.72 +0.4 5,732,280 - BWI76_RS27615 0.89 -0.2 5,732,294 + +0.2 5,732,348 + -0.2 5,732,356 - -0.9 5,732,356 - +0.6 5,732,356 - -0.5 5,732,356 - -0.1 5,732,374 + -2.1 5,732,432 + +0.7 5,732,440 - +0.6 5,732,460 - -1.8 5,732,492 - -0.3 5,732,568 + +0.8 5,732,576 - +1.2 5,732,576 - +0.3 5,732,587 + BWI76_RS27620 0.11 -0.2 5,732,624 - BWI76_RS27620 0.19 +0.7 5,732,631 + BWI76_RS27620 0.21 +0.2 5,732,639 - BWI76_RS27620 0.22 +0.7 5,732,639 - BWI76_RS27620 0.22 -1.2
Or see this region's nucleotide sequence