Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12710

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12705 and BWI76_RS12710 are separated by 43 nucleotidesBWI76_RS12710 and BWI76_RS12715 are separated by 193 nucleotidesBWI76_RS12715 and BWI76_RS12720 are separated by 82 nucleotides BWI76_RS12705: BWI76_RS12705 - ATP-dependent RNA helicase, at 2,648,224 to 2,649,597 _RS12705 BWI76_RS12710: BWI76_RS12710 - tRNA 2-thiocytidine(32) synthetase TtcA, at 2,649,641 to 2,650,576 _RS12710 BWI76_RS12715: BWI76_RS12715 - universal stress protein F, at 2,650,770 to 2,651,204 _RS12715 BWI76_RS12720: BWI76_RS12720 - KTSC domain-containing protein, at 2,651,287 to 2,651,499 _RS12720 Position (kb) 2649 2650 2651Strain fitness (log2 ratio) -2 -1 0 1 2at 2648.662 kb on + strand, within BWI76_RS12705at 2648.673 kb on + strand, within BWI76_RS12705at 2648.815 kb on + strand, within BWI76_RS12705at 2649.044 kb on + strand, within BWI76_RS12705at 2649.044 kb on + strand, within BWI76_RS12705at 2649.533 kb on + strandat 2649.618 kb on - strandat 2649.618 kb on - strandat 2649.766 kb on + strand, within BWI76_RS12710at 2649.825 kb on - strand, within BWI76_RS12710at 2649.843 kb on + strand, within BWI76_RS12710at 2649.878 kb on - strand, within BWI76_RS12710at 2649.944 kb on - strand, within BWI76_RS12710at 2650.099 kb on + strand, within BWI76_RS12710at 2650.159 kb on + strand, within BWI76_RS12710at 2650.198 kb on - strand, within BWI76_RS12710at 2650.426 kb on + strand, within BWI76_RS12710at 2650.673 kb on - strandat 2650.765 kb on - strandat 2650.843 kb on - strand, within BWI76_RS12715at 2651.016 kb on - strand, within BWI76_RS12715at 2651.016 kb on - strand, within BWI76_RS12715at 2651.041 kb on + strand, within BWI76_RS12715at 2651.041 kb on + strand, within BWI76_RS12715at 2651.041 kb on + strand, within BWI76_RS12715at 2651.049 kb on - strand, within BWI76_RS12715at 2651.062 kb on + strand, within BWI76_RS12715at 2651.388 kb on + strand, within BWI76_RS12720at 2651.390 kb on + strand, within BWI76_RS12720at 2651.390 kb on + strand, within BWI76_RS12720at 2651.398 kb on - strand, within BWI76_RS12720at 2651.398 kb on - strand, within BWI76_RS12720at 2651.403 kb on + strand, within BWI76_RS12720at 2651.405 kb on - strand, within BWI76_RS12720at 2651.445 kb on + strand, within BWI76_RS12720at 2651.480 kb on + strandat 2651.488 kb on - strandat 2651.494 kb on + strandat 2651.504 kb on + strandat 2651.527 kb on + strandat 2651.535 kb on - strandat 2651.545 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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2,648,662 + BWI76_RS12705 0.32 -0.2
2,648,673 + BWI76_RS12705 0.33 +1.7
2,648,815 + BWI76_RS12705 0.43 -0.1
2,649,044 + BWI76_RS12705 0.60 -0.2
2,649,044 + BWI76_RS12705 0.60 +0.0
2,649,533 + +0.9
2,649,618 - +0.2
2,649,618 - +2.7
2,649,766 + BWI76_RS12710 0.13 -1.2
2,649,825 - BWI76_RS12710 0.20 -1.1
2,649,843 + BWI76_RS12710 0.22 -0.6
2,649,878 - BWI76_RS12710 0.25 -1.0
2,649,944 - BWI76_RS12710 0.32 -0.5
2,650,099 + BWI76_RS12710 0.49 -0.9
2,650,159 + BWI76_RS12710 0.55 -0.3
2,650,198 - BWI76_RS12710 0.60 -0.3
2,650,426 + BWI76_RS12710 0.84 -0.0
2,650,673 - -0.9
2,650,765 - +0.1
2,650,843 - BWI76_RS12715 0.17 +0.2
2,651,016 - BWI76_RS12715 0.57 -0.1
2,651,016 - BWI76_RS12715 0.57 -0.2
2,651,041 + BWI76_RS12715 0.62 +0.4
2,651,041 + BWI76_RS12715 0.62 +0.3
2,651,041 + BWI76_RS12715 0.62 +1.1
2,651,049 - BWI76_RS12715 0.64 +0.5
2,651,062 + BWI76_RS12715 0.67 -1.0
2,651,388 + BWI76_RS12720 0.47 -0.7
2,651,390 + BWI76_RS12720 0.48 +1.2
2,651,390 + BWI76_RS12720 0.48 -2.4
2,651,398 - BWI76_RS12720 0.52 -0.3
2,651,398 - BWI76_RS12720 0.52 +0.2
2,651,403 + BWI76_RS12720 0.54 -0.2
2,651,405 - BWI76_RS12720 0.55 -0.2
2,651,445 + BWI76_RS12720 0.74 -0.4
2,651,480 + -0.1
2,651,488 - +0.2
2,651,494 + -2.5
2,651,504 + -1.1
2,651,527 + +0.8
2,651,535 - -0.3
2,651,545 - -0.0

Or see this region's nucleotide sequence