Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09285

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09275 and BWI76_RS09280 are separated by 3 nucleotidesBWI76_RS09280 and BWI76_RS09285 are separated by 95 nucleotidesBWI76_RS09285 and BWI76_RS09290 overlap by 1 nucleotidesBWI76_RS09290 and BWI76_RS09295 are separated by 3 nucleotidesBWI76_RS09295 and BWI76_RS09300 overlap by 20 nucleotidesBWI76_RS09300 and BWI76_RS09305 are separated by 4 nucleotides BWI76_RS09275: BWI76_RS09275 - major capsid protein, at 1,953,906 to 1,954,958 _RS09275 BWI76_RS09280: BWI76_RS09280 - terminase endonuclease subunit, at 1,954,962 to 1,955,615 _RS09280 BWI76_RS09285: BWI76_RS09285 - hypothetical protein, at 1,955,711 to 1,956,175 _RS09285 BWI76_RS09290: BWI76_RS09290 - phage Tail Protein X, at 1,956,175 to 1,956,378 _RS09290 BWI76_RS09295: BWI76_RS09295 - hypothetical protein, at 1,956,382 to 1,956,597 _RS09295 BWI76_RS09300: BWI76_RS09300 - phage lysozyme, at 1,956,578 to 1,957,087 _RS09300 BWI76_RS09305: BWI76_RS09305 - hypothetical protein, at 1,957,092 to 1,957,475 _RS09305 Position (kb) 1955 1956 1957Strain fitness (log2 ratio) -1 0 1 2at 1954.908 kb on - strandat 1955.050 kb on + strand, within BWI76_RS09280at 1955.129 kb on + strand, within BWI76_RS09280at 1955.441 kb on + strand, within BWI76_RS09280at 1955.517 kb on + strand, within BWI76_RS09280at 1955.517 kb on + strand, within BWI76_RS09280at 1955.985 kb on - strand, within BWI76_RS09285at 1955.989 kb on + strand, within BWI76_RS09285at 1956.256 kb on - strand, within BWI76_RS09290at 1956.335 kb on - strand, within BWI76_RS09290at 1956.443 kb on - strand, within BWI76_RS09295at 1956.467 kb on + strand, within BWI76_RS09295at 1956.467 kb on + strand, within BWI76_RS09295at 1956.486 kb on + strand, within BWI76_RS09295at 1956.656 kb on + strand, within BWI76_RS09300at 1956.860 kb on + strand, within BWI76_RS09300at 1956.865 kb on + strand, within BWI76_RS09300at 1956.866 kb on - strand, within BWI76_RS09300at 1956.868 kb on - strand, within BWI76_RS09300at 1956.995 kb on - strand, within BWI76_RS09300at 1957.127 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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1,954,908 - +1.0
1,955,050 + BWI76_RS09280 0.13 -0.3
1,955,129 + BWI76_RS09280 0.26 +0.4
1,955,441 + BWI76_RS09280 0.73 +0.6
1,955,517 + BWI76_RS09280 0.85 -0.4
1,955,517 + BWI76_RS09280 0.85 -0.5
1,955,985 - BWI76_RS09285 0.59 -0.8
1,955,989 + BWI76_RS09285 0.60 -0.8
1,956,256 - BWI76_RS09290 0.40 +1.2
1,956,335 - BWI76_RS09290 0.78 -0.4
1,956,443 - BWI76_RS09295 0.28 +0.2
1,956,467 + BWI76_RS09295 0.39 +0.3
1,956,467 + BWI76_RS09295 0.39 -0.9
1,956,486 + BWI76_RS09295 0.48 +2.0
1,956,656 + BWI76_RS09300 0.15 +0.1
1,956,860 + BWI76_RS09300 0.55 +0.5
1,956,865 + BWI76_RS09300 0.56 +1.4
1,956,866 - BWI76_RS09300 0.56 -0.3
1,956,868 - BWI76_RS09300 0.57 -0.2
1,956,995 - BWI76_RS09300 0.82 -0.2
1,957,127 + -1.5

Or see this region's nucleotide sequence