Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02925

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02915 and BWI76_RS02920 are separated by 18 nucleotidesBWI76_RS02920 and BWI76_RS02925 are separated by 25 nucleotidesBWI76_RS02925 and BWI76_RS02930 are separated by 17 nucleotidesBWI76_RS02930 and BWI76_RS02935 are separated by 257 nucleotides BWI76_RS02915: BWI76_RS02915 - Hcp family T6SS protein Cts2H, at 595,729 to 596,214 _RS02915 BWI76_RS02920: BWI76_RS02920 - addiction module toxin RelE, at 596,233 to 596,379 _RS02920 BWI76_RS02925: BWI76_RS02925 - type IV secretion protein Rhs, at 596,405 to 598,123 _RS02925 BWI76_RS02930: BWI76_RS02930 - anaerobic ribonucleoside-triphosphate reductase activating protein, at 598,141 to 598,605 _RS02930 BWI76_RS02935: BWI76_RS02935 - anaerobic ribonucleoside triphosphate reductase, at 598,863 to 601,001 _RS02935 Position (kb) 596 597 598 599Strain fitness (log2 ratio) -2 -1 0 1 2at 595.444 kb on + strandat 595.444 kb on + strandat 595.444 kb on + strandat 595.444 kb on + strandat 595.444 kb on + strandat 595.452 kb on - strandat 595.452 kb on - strandat 595.500 kb on + strandat 595.508 kb on - strandat 595.508 kb on - strandat 595.545 kb on + strandat 595.545 kb on + strandat 595.545 kb on + strandat 595.546 kb on + strandat 595.546 kb on + strandat 595.546 kb on + strandat 595.546 kb on + strandat 595.546 kb on + strandat 595.546 kb on + strandat 595.546 kb on + strandat 595.554 kb on - strandat 595.554 kb on - strandat 595.554 kb on - strandat 595.598 kb on - strandat 595.612 kb on - strandat 595.623 kb on + strandat 595.623 kb on + strandat 595.623 kb on + strandat 595.623 kb on + strandat 595.623 kb on + strandat 595.680 kb on + strandat 595.680 kb on + strandat 595.680 kb on + strandat 595.680 kb on + strandat 595.680 kb on + strandat 595.680 kb on + strandat 595.680 kb on + strandat 595.690 kb on + strandat 595.698 kb on - strandat 595.700 kb on + strandat 595.700 kb on + strandat 595.700 kb on + strandat 595.700 kb on + strandat 595.700 kb on + strandat 595.700 kb on + strandat 595.708 kb on - strandat 595.708 kb on - strandat 595.718 kb on - strandat 595.720 kb on + strandat 595.720 kb on + strandat 595.722 kb on + strandat 595.765 kb on + strandat 595.765 kb on + strandat 595.774 kb on - strandat 595.776 kb on + strandat 595.784 kb on - strand, within BWI76_RS02915at 595.814 kb on + strandat 595.817 kb on + strand, within BWI76_RS02915at 595.817 kb on + strand, within BWI76_RS02915at 595.817 kb on + strand, within BWI76_RS02915at 595.817 kb on + strand, within BWI76_RS02915at 595.817 kb on + strand, within BWI76_RS02915at 595.825 kb on - strand, within BWI76_RS02915at 595.825 kb on - strand, within BWI76_RS02915at 595.825 kb on - strand, within BWI76_RS02915at 595.851 kb on + strand, within BWI76_RS02915at 595.851 kb on + strand, within BWI76_RS02915at 595.869 kb on - strand, within BWI76_RS02915at 595.932 kb on + strand, within BWI76_RS02915at 595.939 kb on + strand, within BWI76_RS02915at 595.941 kb on + strand, within BWI76_RS02915at 595.947 kb on - strand, within BWI76_RS02915at 596.048 kb on + strand, within BWI76_RS02915at 596.215 kb on + strandat 596.216 kb on + strandat 596.223 kb on - strandat 596.231 kb on - strandat 596.239 kb on - strandat 596.332 kb on + strand, within BWI76_RS02920at 596.427 kb on + strandat 596.638 kb on + strand, within BWI76_RS02925at 596.803 kb on - strand, within BWI76_RS02925at 596.895 kb on + strand, within BWI76_RS02925at 596.972 kb on + strand, within BWI76_RS02925at 597.121 kb on + strand, within BWI76_RS02925at 597.213 kb on + strand, within BWI76_RS02925at 597.253 kb on - strand, within BWI76_RS02925at 597.280 kb on + strand, within BWI76_RS02925at 597.280 kb on + strand, within BWI76_RS02925at 597.534 kb on + strand, within BWI76_RS02925at 597.544 kb on - strand, within BWI76_RS02925at 597.717 kb on - strand, within BWI76_RS02925at 597.744 kb on - strand, within BWI76_RS02925at 597.770 kb on - strand, within BWI76_RS02925at 597.921 kb on - strand, within BWI76_RS02925at 598.153 kb on - strandat 598.281 kb on + strand, within BWI76_RS02930at 598.521 kb on + strand, within BWI76_RS02930at 598.552 kb on + strand, within BWI76_RS02930at 598.559 kb on + strandat 598.591 kb on - strandat 598.853 kb on - strandat 598.924 kb on + strandat 599.019 kb on + strandat 599.027 kb on - strandat 599.118 kb on + strand, within BWI76_RS02935

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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595,444 + +0.4
595,444 + -1.5
595,444 + -0.1
595,444 + -0.5
595,444 + -0.7
595,452 - +0.9
595,452 - -0.4
595,500 + -0.5
595,508 - +2.1
595,508 - +0.1
595,545 + +0.2
595,545 + -1.1
595,545 + +0.3
595,546 + -0.3
595,546 + +0.5
595,546 + +0.5
595,546 + +0.3
595,546 + +0.6
595,546 + +0.6
595,546 + -1.5
595,554 - +0.1
595,554 - -1.1
595,554 - -0.1
595,598 - +0.7
595,612 - -0.5
595,623 + -1.4
595,623 + -0.7
595,623 + -0.8
595,623 + -0.3
595,623 + +1.1
595,680 + -0.4
595,680 + -1.7
595,680 + -0.2
595,680 + -0.2
595,680 + +0.4
595,680 + +0.6
595,680 + -0.4
595,690 + -0.1
595,698 - +0.9
595,700 + +0.0
595,700 + -0.2
595,700 + +0.2
595,700 + -0.1
595,700 + -0.8
595,700 + -0.4
595,708 - +0.5
595,708 - -0.8
595,718 - +0.3
595,720 + +0.4
595,720 + +0.0
595,722 + -1.0
595,765 + +0.1
595,765 + +0.2
595,774 - +0.5
595,776 + +1.1
595,784 - BWI76_RS02915 0.11 +0.3
595,814 + -0.3
595,817 + BWI76_RS02915 0.18 -0.8
595,817 + BWI76_RS02915 0.18 -1.4
595,817 + BWI76_RS02915 0.18 +0.1
595,817 + BWI76_RS02915 0.18 -0.1
595,817 + BWI76_RS02915 0.18 -1.8
595,825 - BWI76_RS02915 0.20 -0.6
595,825 - BWI76_RS02915 0.20 +2.5
595,825 - BWI76_RS02915 0.20 -1.4
595,851 + BWI76_RS02915 0.25 -0.2
595,851 + BWI76_RS02915 0.25 -0.5
595,869 - BWI76_RS02915 0.29 -1.1
595,932 + BWI76_RS02915 0.42 -0.4
595,939 + BWI76_RS02915 0.43 -0.4
595,941 + BWI76_RS02915 0.44 -0.1
595,947 - BWI76_RS02915 0.45 -0.1
596,048 + BWI76_RS02915 0.66 +0.7
596,215 + -1.2
596,216 + -0.1
596,223 - +0.6
596,231 - +2.1
596,239 - -0.3
596,332 + BWI76_RS02920 0.67 +0.2
596,427 + -0.7
596,638 + BWI76_RS02925 0.14 +1.1
596,803 - BWI76_RS02925 0.23 -0.4
596,895 + BWI76_RS02925 0.29 +0.1
596,972 + BWI76_RS02925 0.33 +0.1
597,121 + BWI76_RS02925 0.42 +1.1
597,213 + BWI76_RS02925 0.47 -1.5
597,253 - BWI76_RS02925 0.49 +0.6
597,280 + BWI76_RS02925 0.51 +0.3
597,280 + BWI76_RS02925 0.51 -0.2
597,534 + BWI76_RS02925 0.66 -0.1
597,544 - BWI76_RS02925 0.66 -0.4
597,717 - BWI76_RS02925 0.76 +0.1
597,744 - BWI76_RS02925 0.78 +0.3
597,770 - BWI76_RS02925 0.79 -0.2
597,921 - BWI76_RS02925 0.88 +1.1
598,153 - +0.3
598,281 + BWI76_RS02930 0.30 +0.0
598,521 + BWI76_RS02930 0.82 -0.0
598,552 + BWI76_RS02930 0.88 +0.2
598,559 + -0.7
598,591 - -0.2
598,853 - -0.0
598,924 + +1.8
599,019 + -1.0
599,027 - -0.1
599,118 + BWI76_RS02935 0.12 +0.8

Or see this region's nucleotide sequence