Experiment: Nitrate reduction with yeast extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_28935 and AO356_28940 are separated by 185 nucleotides AO356_28940 and AO356_28945 are separated by 41 nucleotides AO356_28945 and AO356_28950 are separated by 126 nucleotides
AO356_28935: AO356_28935 - FMN-dependent NADH-azoreductase, at 6,728,458 to 6,729,126
_28935
AO356_28940: AO356_28940 - LysR family transcriptional regulator, at 6,729,312 to 6,730,253
_28940
AO356_28945: AO356_28945 - aminomethyltransferase, at 6,730,295 to 6,730,909
_28945
AO356_28950: AO356_28950 - LysR family transcriptional regulator, at 6,731,036 to 6,731,926
_28950
Position (kb)
6729
6730
6731 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6728.331 kb on - strand at 6728.425 kb on + strand at 6728.475 kb on + strand at 6728.747 kb on - strand, within AO356_28935 at 6728.747 kb on - strand, within AO356_28935 at 6728.747 kb on - strand, within AO356_28935 at 6728.747 kb on - strand, within AO356_28935 at 6728.747 kb on - strand, within AO356_28935 at 6728.752 kb on + strand, within AO356_28935 at 6728.752 kb on + strand, within AO356_28935 at 6728.752 kb on + strand, within AO356_28935 at 6728.753 kb on - strand, within AO356_28935 at 6728.753 kb on - strand, within AO356_28935 at 6728.753 kb on - strand, within AO356_28935 at 6728.753 kb on - strand, within AO356_28935 at 6728.753 kb on - strand, within AO356_28935 at 6728.753 kb on - strand, within AO356_28935 at 6728.772 kb on + strand, within AO356_28935 at 6728.772 kb on + strand, within AO356_28935 at 6728.814 kb on + strand, within AO356_28935 at 6728.883 kb on - strand, within AO356_28935 at 6728.971 kb on + strand, within AO356_28935 at 6728.972 kb on - strand, within AO356_28935 at 6728.972 kb on - strand, within AO356_28935 at 6729.134 kb on + strand at 6729.297 kb on + strand at 6729.298 kb on - strand at 6729.298 kb on - strand at 6729.298 kb on - strand at 6729.298 kb on - strand at 6729.441 kb on + strand, within AO356_28940 at 6729.442 kb on - strand, within AO356_28940 at 6729.442 kb on - strand, within AO356_28940 at 6729.522 kb on + strand, within AO356_28940 at 6729.523 kb on - strand, within AO356_28940 at 6729.523 kb on - strand, within AO356_28940 at 6729.523 kb on - strand, within AO356_28940 at 6729.769 kb on + strand, within AO356_28940 at 6729.769 kb on + strand, within AO356_28940 at 6729.769 kb on + strand, within AO356_28940 at 6729.769 kb on + strand, within AO356_28940 at 6729.769 kb on + strand, within AO356_28940 at 6729.902 kb on - strand, within AO356_28940 at 6729.902 kb on - strand, within AO356_28940 at 6729.999 kb on + strand, within AO356_28940 at 6730.000 kb on - strand, within AO356_28940 at 6730.051 kb on + strand, within AO356_28940 at 6730.052 kb on - strand, within AO356_28940 at 6730.278 kb on + strand at 6730.279 kb on - strand at 6730.308 kb on + strand at 6730.338 kb on - strand at 6730.346 kb on - strand at 6730.346 kb on - strand at 6730.346 kb on - strand at 6730.396 kb on + strand, within AO356_28945 at 6730.407 kb on + strand, within AO356_28945 at 6730.407 kb on + strand, within AO356_28945 at 6730.407 kb on + strand, within AO356_28945 at 6730.408 kb on - strand, within AO356_28945 at 6730.408 kb on - strand, within AO356_28945 at 6730.549 kb on + strand, within AO356_28945 at 6730.549 kb on + strand, within AO356_28945 at 6730.649 kb on - strand, within AO356_28945 at 6730.780 kb on + strand, within AO356_28945 at 6730.895 kb on - strand at 6730.898 kb on + strand at 6730.931 kb on + strand at 6730.996 kb on + strand at 6730.996 kb on + strand at 6730.997 kb on - strand at 6731.010 kb on + strand at 6731.010 kb on + strand at 6731.012 kb on + strand at 6731.130 kb on + strand, within AO356_28950
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction with yeast extract remove 6,728,331 - +0.3 6,728,425 + -0.4 6,728,475 + +0.8 6,728,747 - AO356_28935 0.43 -1.0 6,728,747 - AO356_28935 0.43 +0.7 6,728,747 - AO356_28935 0.43 -2.5 6,728,747 - AO356_28935 0.43 +0.8 6,728,747 - AO356_28935 0.43 -1.6 6,728,752 + AO356_28935 0.44 +0.3 6,728,752 + AO356_28935 0.44 +0.5 6,728,752 + AO356_28935 0.44 +0.7 6,728,753 - AO356_28935 0.44 -0.6 6,728,753 - AO356_28935 0.44 -1.5 6,728,753 - AO356_28935 0.44 -0.2 6,728,753 - AO356_28935 0.44 -0.8 6,728,753 - AO356_28935 0.44 -0.1 6,728,753 - AO356_28935 0.44 +0.7 6,728,772 + AO356_28935 0.47 +0.7 6,728,772 + AO356_28935 0.47 -1.1 6,728,814 + AO356_28935 0.53 -1.5 6,728,883 - AO356_28935 0.64 -1.4 6,728,971 + AO356_28935 0.77 +0.4 6,728,972 - AO356_28935 0.77 -1.7 6,728,972 - AO356_28935 0.77 -2.3 6,729,134 + +1.6 6,729,297 + +1.8 6,729,298 - +3.5 6,729,298 - +0.3 6,729,298 - -1.7 6,729,298 - -0.6 6,729,441 + AO356_28940 0.14 +0.6 6,729,442 - AO356_28940 0.14 +2.5 6,729,442 - AO356_28940 0.14 +1.9 6,729,522 + AO356_28940 0.22 -1.5 6,729,523 - AO356_28940 0.22 +0.1 6,729,523 - AO356_28940 0.22 +0.2 6,729,523 - AO356_28940 0.22 -1.2 6,729,769 + AO356_28940 0.49 -0.3 6,729,769 + AO356_28940 0.49 -1.2 6,729,769 + AO356_28940 0.49 -0.1 6,729,769 + AO356_28940 0.49 -2.2 6,729,769 + AO356_28940 0.49 -1.4 6,729,902 - AO356_28940 0.63 +1.7 6,729,902 - AO356_28940 0.63 +0.8 6,729,999 + AO356_28940 0.73 +1.2 6,730,000 - AO356_28940 0.73 -1.3 6,730,051 + AO356_28940 0.78 +3.3 6,730,052 - AO356_28940 0.79 +1.3 6,730,278 + -2.1 6,730,279 - -3.1 6,730,308 + -1.7 6,730,338 - +0.4 6,730,346 - +0.5 6,730,346 - -0.5 6,730,346 - +0.5 6,730,396 + AO356_28945 0.16 -1.1 6,730,407 + AO356_28945 0.18 +1.9 6,730,407 + AO356_28945 0.18 +1.1 6,730,407 + AO356_28945 0.18 +0.1 6,730,408 - AO356_28945 0.18 +1.2 6,730,408 - AO356_28945 0.18 +2.0 6,730,549 + AO356_28945 0.41 -0.9 6,730,549 + AO356_28945 0.41 +0.7 6,730,649 - AO356_28945 0.58 -0.3 6,730,780 + AO356_28945 0.79 -0.1 6,730,895 - +1.1 6,730,898 + -0.8 6,730,931 + +0.8 6,730,996 + -1.5 6,730,996 + -0.7 6,730,997 - +1.6 6,731,010 + +0.5 6,731,010 + -0.1 6,731,012 + +0.9 6,731,130 + AO356_28950 0.11 -1.7
Or see this region's nucleotide sequence