Experiment: Nitrate reduction with yeast extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_28035 and AO356_28040 are separated by 16 nucleotides AO356_28040 and AO356_28045 are separated by 10 nucleotides AO356_28045 and AO356_28050 are separated by 5 nucleotides
AO356_28035: AO356_28035 - MBL fold metallo-hydrolase, at 6,403,004 to 6,403,942
_28035
AO356_28040: AO356_28040 - quinoprotein dehydrogenase-associated SoxYZ-like carrier, at 6,403,959 to 6,404,714
_28040
AO356_28045: AO356_28045 - amino acid ABC transporter substrate-binding protein, at 6,404,725 to 6,405,600
_28045
AO356_28050: AO356_28050 - cytochrome c-550 PedF, at 6,405,606 to 6,406,067
_28050
Position (kb)
6403
6404
6405 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6403.059 kb on + strand at 6403.075 kb on + strand at 6403.090 kb on - strand at 6403.337 kb on - strand, within AO356_28035 at 6403.379 kb on + strand, within AO356_28035 at 6403.379 kb on + strand, within AO356_28035 at 6403.540 kb on - strand, within AO356_28035 at 6403.637 kb on + strand, within AO356_28035 at 6403.637 kb on + strand, within AO356_28035 at 6403.638 kb on - strand, within AO356_28035 at 6403.638 kb on - strand, within AO356_28035 at 6403.638 kb on - strand, within AO356_28035 at 6404.113 kb on - strand, within AO356_28040 at 6404.113 kb on - strand, within AO356_28040 at 6404.113 kb on - strand, within AO356_28040 at 6404.726 kb on + strand at 6404.732 kb on + strand at 6404.732 kb on + strand at 6404.733 kb on - strand at 6404.733 kb on - strand at 6405.077 kb on + strand, within AO356_28045 at 6405.077 kb on + strand, within AO356_28045 at 6405.167 kb on + strand, within AO356_28045 at 6405.167 kb on + strand, within AO356_28045 at 6405.168 kb on - strand, within AO356_28045 at 6405.168 kb on - strand, within AO356_28045 at 6405.309 kb on - strand, within AO356_28045 at 6405.332 kb on + strand, within AO356_28045 at 6405.332 kb on + strand, within AO356_28045 at 6405.332 kb on + strand, within AO356_28045 at 6405.332 kb on + strand, within AO356_28045 at 6405.332 kb on + strand, within AO356_28045 at 6405.332 kb on + strand, within AO356_28045 at 6405.332 kb on + strand, within AO356_28045 at 6405.332 kb on + strand, within AO356_28045 at 6405.486 kb on - strand, within AO356_28045 at 6405.635 kb on - strand at 6405.635 kb on - strand at 6405.670 kb on + strand, within AO356_28050 at 6405.691 kb on + strand, within AO356_28050 at 6405.692 kb on - strand, within AO356_28050
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction with yeast extract remove 6,403,059 + -0.1 6,403,075 + +1.0 6,403,090 - +1.8 6,403,337 - AO356_28035 0.35 -1.3 6,403,379 + AO356_28035 0.40 -0.7 6,403,379 + AO356_28035 0.40 +1.8 6,403,540 - AO356_28035 0.57 +0.2 6,403,637 + AO356_28035 0.67 -1.3 6,403,637 + AO356_28035 0.67 -0.8 6,403,638 - AO356_28035 0.68 -1.8 6,403,638 - AO356_28035 0.68 -2.1 6,403,638 - AO356_28035 0.68 -0.5 6,404,113 - AO356_28040 0.20 +0.0 6,404,113 - AO356_28040 0.20 -1.0 6,404,113 - AO356_28040 0.20 -2.4 6,404,726 + +0.7 6,404,732 + -0.2 6,404,732 + +0.9 6,404,733 - -2.0 6,404,733 - +0.5 6,405,077 + AO356_28045 0.40 -1.0 6,405,077 + AO356_28045 0.40 +0.0 6,405,167 + AO356_28045 0.50 +0.4 6,405,167 + AO356_28045 0.50 +1.1 6,405,168 - AO356_28045 0.51 -1.0 6,405,168 - AO356_28045 0.51 -0.2 6,405,309 - AO356_28045 0.67 +1.0 6,405,332 + AO356_28045 0.69 -1.7 6,405,332 + AO356_28045 0.69 -1.6 6,405,332 + AO356_28045 0.69 +1.0 6,405,332 + AO356_28045 0.69 -0.1 6,405,332 + AO356_28045 0.69 -1.2 6,405,332 + AO356_28045 0.69 +0.7 6,405,332 + AO356_28045 0.69 +0.8 6,405,332 + AO356_28045 0.69 -0.1 6,405,486 - AO356_28045 0.87 -2.0 6,405,635 - -2.6 6,405,635 - +0.1 6,405,670 + AO356_28050 0.14 -0.0 6,405,691 + AO356_28050 0.18 -1.0 6,405,692 - AO356_28050 0.19 +1.8
Or see this region's nucleotide sequence